DNA as a dietary biomarker in Antarctic krill, Euphausia superba
The diet of Antarctic krill (Euphausia superba) has been studied using a variety of techniques, but current methods still suffer from problems that are difficult to solve. This study examined an alternative approach utilizing DNA as a prey biomarker. Methods were developed for the preservation, extr...
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ftunivtasecite:oai:ecite.utas.edu.au:42239 2023-05-15T13:59:47+02:00 DNA as a dietary biomarker in Antarctic krill, Euphausia superba Passmore, A Jarman, SN Swadling, KM Kawaguchi, SR McMinn, A Nicol, S 2006 https://doi.org/10.1007/s10126-005-6088-8 http://www.ncbi.nlm.nih.gov/pubmed/16924375 http://ecite.utas.edu.au/42239 en eng Springer http://dx.doi.org/10.1007/s10126-005-6088-8 Passmore, A and Jarman, SN and Swadling, KM and Kawaguchi, SR and McMinn, A and Nicol, S, DNA as a dietary biomarker in Antarctic krill, Euphausia superba , Marine Biotechnology, 8, (6) pp. 686-696. ISSN 1436-2228 (2006) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/16924375 http://ecite.utas.edu.au/42239 Biological Sciences Zoology Invertebrate biology Refereed Article PeerReviewed 2006 ftunivtasecite https://doi.org/10.1007/s10126-005-6088-8 2022-04-04T22:16:42Z The diet of Antarctic krill (Euphausia superba) has been studied using a variety of techniques, but current methods still suffer from problems that are difficult to solve. This study examined an alternative approach utilizing DNA as a prey biomarker. Methods were developed for the preservation, extraction, and identification of prey DNA from krill collected in the field. Group-specific polymerase chain reaction (PCR) was used to amplify diatom prey (Phylum: Bacillariophyta) and the results from DNA clone libraries were compared with microscopic diet analysis. DNA analysis was superior to microscopy for prey detection. However, differences in prey relative abundance estimates between the two techniques suggested some bias in the DNA-based estimates. Quantification showed that large amounts of prey DNA had been successfully preserved and extracted. Overall the results suggest that the application of DNA-based diet analysis to krill warrants further investigation, particularly for prey that are difficult to study using other methods. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba eCite UTAS (University of Tasmania) Antarctic Marine Biotechnology 8 6 686 696 |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
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English |
topic |
Biological Sciences Zoology Invertebrate biology |
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Biological Sciences Zoology Invertebrate biology Passmore, A Jarman, SN Swadling, KM Kawaguchi, SR McMinn, A Nicol, S DNA as a dietary biomarker in Antarctic krill, Euphausia superba |
topic_facet |
Biological Sciences Zoology Invertebrate biology |
description |
The diet of Antarctic krill (Euphausia superba) has been studied using a variety of techniques, but current methods still suffer from problems that are difficult to solve. This study examined an alternative approach utilizing DNA as a prey biomarker. Methods were developed for the preservation, extraction, and identification of prey DNA from krill collected in the field. Group-specific polymerase chain reaction (PCR) was used to amplify diatom prey (Phylum: Bacillariophyta) and the results from DNA clone libraries were compared with microscopic diet analysis. DNA analysis was superior to microscopy for prey detection. However, differences in prey relative abundance estimates between the two techniques suggested some bias in the DNA-based estimates. Quantification showed that large amounts of prey DNA had been successfully preserved and extracted. Overall the results suggest that the application of DNA-based diet analysis to krill warrants further investigation, particularly for prey that are difficult to study using other methods. |
format |
Article in Journal/Newspaper |
author |
Passmore, A Jarman, SN Swadling, KM Kawaguchi, SR McMinn, A Nicol, S |
author_facet |
Passmore, A Jarman, SN Swadling, KM Kawaguchi, SR McMinn, A Nicol, S |
author_sort |
Passmore, A |
title |
DNA as a dietary biomarker in Antarctic krill, Euphausia superba |
title_short |
DNA as a dietary biomarker in Antarctic krill, Euphausia superba |
title_full |
DNA as a dietary biomarker in Antarctic krill, Euphausia superba |
title_fullStr |
DNA as a dietary biomarker in Antarctic krill, Euphausia superba |
title_full_unstemmed |
DNA as a dietary biomarker in Antarctic krill, Euphausia superba |
title_sort |
dna as a dietary biomarker in antarctic krill, euphausia superba |
publisher |
Springer |
publishDate |
2006 |
url |
https://doi.org/10.1007/s10126-005-6088-8 http://www.ncbi.nlm.nih.gov/pubmed/16924375 http://ecite.utas.edu.au/42239 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba |
op_relation |
http://dx.doi.org/10.1007/s10126-005-6088-8 Passmore, A and Jarman, SN and Swadling, KM and Kawaguchi, SR and McMinn, A and Nicol, S, DNA as a dietary biomarker in Antarctic krill, Euphausia superba , Marine Biotechnology, 8, (6) pp. 686-696. ISSN 1436-2228 (2006) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/16924375 http://ecite.utas.edu.au/42239 |
op_doi |
https://doi.org/10.1007/s10126-005-6088-8 |
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Marine Biotechnology |
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