Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon
Amoebic gill disease (AGD), the most serious infectious disease affecting farmed salmon in Tasmania, is caused by free-living marine amoeba Neoparamoeba sp. The parasites on the gills induce proliferation of epithelial cells initiating a hyperplastic response and reducing the surface area available...
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ftunivtasecite:oai:ecite.utas.edu.au:38294 2023-05-15T15:32:44+02:00 Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon Villavedra, M McCarthy, K To, J Morrison, RN Crosbie, PBB Broady, K Raison, R 2005 https://doi.org/10.1016/j.ijpara.2005.05.014 http://www.ncbi.nlm.nih.gov/pubmed/16102771 http://ecite.utas.edu.au/38294 en eng Elsevier http://dx.doi.org/10.1016/j.ijpara.2005.05.014 Villavedra, M and McCarthy, K and To, J and Morrison, RN and Crosbie, PBB and Broady, K and Raison, R, Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon, International Journal for Parasitology, 35, (13) pp. 1417-1423. ISSN 0020-7519 (2005) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/16102771 http://ecite.utas.edu.au/38294 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Refereed Article PeerReviewed 2005 ftunivtasecite https://doi.org/10.1016/j.ijpara.2005.05.014 2019-12-13T21:16:00Z Amoebic gill disease (AGD), the most serious infectious disease affecting farmed salmon in Tasmania, is caused by free-living marine amoeba Neoparamoeba sp. The parasites on the gills induce proliferation of epithelial cells initiating a hyperplastic response and reducing the surface area available for gaseous exchange. AGD can be induced in salmon by exposure to freshly isolated Neoparamoeba from AGD infected fish, however cultured Neoparamoeba are non-infective. We describe here antigenic differences between freshly isolated and in vitro cultured parasites, and within individual isolates of the parasite cultured under different conditions. Immunoblot analysis using polyclonal antisera, revealed differences in the antigen profiles of two cultured isolates of Neoparamoeba sp. when they were grown on agar versus in liquid medium. However, the antigen profiles of the two isolates were very similar when they were grown under the same culture conditions. Comparison of these antigen profiles with a preparation from parasites freshly isolated from infected gills revealed a very limited number of shared antigens. In addition monoclonal antibodies (mAbs) raised against surface antigens of cultured parasites were used in an indirect immunofluorescence assay to assess the expression of specific surface antigens of Neoparamoeba sp. after various periods in culture. Significant changes in antigen expression of freshly isolated parasites were observed after 15 days of in vitro culture. The use of mAb demonstrated progressive exposure/expression of individual antigens on the surface of the freshly isolated parasites during the period in culture. 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Atlantic salmon eCite UTAS (University of Tasmania) International Journal for Parasitology 35 13 1417 1423 |
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Open Polar |
collection |
eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
spellingShingle |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Villavedra, M McCarthy, K To, J Morrison, RN Crosbie, PBB Broady, K Raison, R Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
description |
Amoebic gill disease (AGD), the most serious infectious disease affecting farmed salmon in Tasmania, is caused by free-living marine amoeba Neoparamoeba sp. The parasites on the gills induce proliferation of epithelial cells initiating a hyperplastic response and reducing the surface area available for gaseous exchange. AGD can be induced in salmon by exposure to freshly isolated Neoparamoeba from AGD infected fish, however cultured Neoparamoeba are non-infective. We describe here antigenic differences between freshly isolated and in vitro cultured parasites, and within individual isolates of the parasite cultured under different conditions. Immunoblot analysis using polyclonal antisera, revealed differences in the antigen profiles of two cultured isolates of Neoparamoeba sp. when they were grown on agar versus in liquid medium. However, the antigen profiles of the two isolates were very similar when they were grown under the same culture conditions. Comparison of these antigen profiles with a preparation from parasites freshly isolated from infected gills revealed a very limited number of shared antigens. In addition monoclonal antibodies (mAbs) raised against surface antigens of cultured parasites were used in an indirect immunofluorescence assay to assess the expression of specific surface antigens of Neoparamoeba sp. after various periods in culture. Significant changes in antigen expression of freshly isolated parasites were observed after 15 days of in vitro culture. The use of mAb demonstrated progressive exposure/expression of individual antigens on the surface of the freshly isolated parasites during the period in culture. 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Villavedra, M McCarthy, K To, J Morrison, RN Crosbie, PBB Broady, K Raison, R |
author_facet |
Villavedra, M McCarthy, K To, J Morrison, RN Crosbie, PBB Broady, K Raison, R |
author_sort |
Villavedra, M |
title |
Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon |
title_short |
Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon |
title_full |
Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon |
title_fullStr |
Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon |
title_full_unstemmed |
Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon |
title_sort |
changes in antigenic profile during culture of neoparamoeba sp., causative agent of amoebic gill disease in atlantic salmon |
publisher |
Elsevier |
publishDate |
2005 |
url |
https://doi.org/10.1016/j.ijpara.2005.05.014 http://www.ncbi.nlm.nih.gov/pubmed/16102771 http://ecite.utas.edu.au/38294 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://dx.doi.org/10.1016/j.ijpara.2005.05.014 Villavedra, M and McCarthy, K and To, J and Morrison, RN and Crosbie, PBB and Broady, K and Raison, R, Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon, International Journal for Parasitology, 35, (13) pp. 1417-1423. ISSN 0020-7519 (2005) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/16102771 http://ecite.utas.edu.au/38294 |
op_doi |
https://doi.org/10.1016/j.ijpara.2005.05.014 |
container_title |
International Journal for Parasitology |
container_volume |
35 |
container_issue |
13 |
container_start_page |
1417 |
op_container_end_page |
1423 |
_version_ |
1766363217603330048 |