Cultured gill-derived Neoparamoeba pemaquidensis fails to elicit amoebic gill disease (AGD) in Atlantic salmon Salmo salar
Amoebic gill disease (AGD) affects the culture of Atlantic salmon Salmo salar in the southeast of Tasmania. The disease is characterised by the presence of epizoic Neoparamoeba spp. in association with hyperplastic gill tissue. Gill-associated amoebae trophozoites were positively selected by plastic...
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ftunivtasecite:oai:ecite.utas.edu.au:36004 2023-05-15T15:30:52+02:00 Cultured gill-derived Neoparamoeba pemaquidensis fails to elicit amoebic gill disease (AGD) in Atlantic salmon Salmo salar Morrison, RN Crosbie, PBB Cook, MT Adams, MB Nowak, BF 2005 https://doi.org/10.3354/dao066135 http://www.ncbi.nlm.nih.gov/pubmed/16231639 http://ecite.utas.edu.au/36004 en eng Inter-Research http://dx.doi.org/10.3354/dao066135 Morrison, RN and Crosbie, PBB and Cook, MT and Adams, MB and Nowak, BF, Cultured gill-derived Neoparamoeba pemaquidensis fails to elicit amoebic gill disease (AGD) in Atlantic salmon Salmo salar , Diseases of Aquatic Organisms, 66, (2) pp. 135-144. ISSN 0177-5103 (2005) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/16231639 http://ecite.utas.edu.au/36004 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Refereed Article PeerReviewed 2005 ftunivtasecite https://doi.org/10.3354/dao066135 2019-12-13T21:14:21Z Amoebic gill disease (AGD) affects the culture of Atlantic salmon Salmo salar in the southeast of Tasmania. The disease is characterised by the presence of epizoic Neoparamoeba spp. in association with hyperplastic gill tissue. Gill-associated amoebae trophozoites were positively selected by plastic adherence for culture in seawater, where they proliferated using heat-killed E. coli as a nutrient source. One isolate of gill-harvested amoebae designated NP251002 was morphologically consistent to N. pemaquidensis under light, fluorescence and transmission electron microscopy. Rabbit anti-N. pemaquidensis antiserum bound to NP251002, and N. pemaquidensis small subunit (SSU) ribosomal DNA (18S rDNA) was detected in NP251002 genomic DNA preparations using PCR. A high degree of similarity in the alignment of the NP251002 18S rDNA PCR amplicon sequence with reference isolates of N. pemaquidensis suggested conspecificity. While short-term culture (72 h) of gill-harvested amoebae does not affect the capacity of amoebae to induce AGD, Atlantic salmon challenged with NP251002 after the trophozoites had been 34 and 98 d in culture exhibited neither gross nor histological evidence of AGD. It is not known if NP251002 were avirulent at the time of isolation, had down-regulated putative virulence factors or virulence was inhibited by the culture conditions. Therefore, the time in culture could be a limiting factor in maintaining virulence using the culture technique described here. Inter-Research 2005. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Diseases of Aquatic Organisms 66 135 144 |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
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English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
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Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Morrison, RN Crosbie, PBB Cook, MT Adams, MB Nowak, BF Cultured gill-derived Neoparamoeba pemaquidensis fails to elicit amoebic gill disease (AGD) in Atlantic salmon Salmo salar |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
description |
Amoebic gill disease (AGD) affects the culture of Atlantic salmon Salmo salar in the southeast of Tasmania. The disease is characterised by the presence of epizoic Neoparamoeba spp. in association with hyperplastic gill tissue. Gill-associated amoebae trophozoites were positively selected by plastic adherence for culture in seawater, where they proliferated using heat-killed E. coli as a nutrient source. One isolate of gill-harvested amoebae designated NP251002 was morphologically consistent to N. pemaquidensis under light, fluorescence and transmission electron microscopy. Rabbit anti-N. pemaquidensis antiserum bound to NP251002, and N. pemaquidensis small subunit (SSU) ribosomal DNA (18S rDNA) was detected in NP251002 genomic DNA preparations using PCR. A high degree of similarity in the alignment of the NP251002 18S rDNA PCR amplicon sequence with reference isolates of N. pemaquidensis suggested conspecificity. While short-term culture (72 h) of gill-harvested amoebae does not affect the capacity of amoebae to induce AGD, Atlantic salmon challenged with NP251002 after the trophozoites had been 34 and 98 d in culture exhibited neither gross nor histological evidence of AGD. It is not known if NP251002 were avirulent at the time of isolation, had down-regulated putative virulence factors or virulence was inhibited by the culture conditions. Therefore, the time in culture could be a limiting factor in maintaining virulence using the culture technique described here. Inter-Research 2005. |
format |
Article in Journal/Newspaper |
author |
Morrison, RN Crosbie, PBB Cook, MT Adams, MB Nowak, BF |
author_facet |
Morrison, RN Crosbie, PBB Cook, MT Adams, MB Nowak, BF |
author_sort |
Morrison, RN |
title |
Cultured gill-derived Neoparamoeba pemaquidensis fails to elicit amoebic gill disease (AGD) in Atlantic salmon Salmo salar |
title_short |
Cultured gill-derived Neoparamoeba pemaquidensis fails to elicit amoebic gill disease (AGD) in Atlantic salmon Salmo salar |
title_full |
Cultured gill-derived Neoparamoeba pemaquidensis fails to elicit amoebic gill disease (AGD) in Atlantic salmon Salmo salar |
title_fullStr |
Cultured gill-derived Neoparamoeba pemaquidensis fails to elicit amoebic gill disease (AGD) in Atlantic salmon Salmo salar |
title_full_unstemmed |
Cultured gill-derived Neoparamoeba pemaquidensis fails to elicit amoebic gill disease (AGD) in Atlantic salmon Salmo salar |
title_sort |
cultured gill-derived neoparamoeba pemaquidensis fails to elicit amoebic gill disease (agd) in atlantic salmon salmo salar |
publisher |
Inter-Research |
publishDate |
2005 |
url |
https://doi.org/10.3354/dao066135 http://www.ncbi.nlm.nih.gov/pubmed/16231639 http://ecite.utas.edu.au/36004 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://dx.doi.org/10.3354/dao066135 Morrison, RN and Crosbie, PBB and Cook, MT and Adams, MB and Nowak, BF, Cultured gill-derived Neoparamoeba pemaquidensis fails to elicit amoebic gill disease (AGD) in Atlantic salmon Salmo salar , Diseases of Aquatic Organisms, 66, (2) pp. 135-144. ISSN 0177-5103 (2005) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/16231639 http://ecite.utas.edu.au/36004 |
op_doi |
https://doi.org/10.3354/dao066135 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
66 |
container_start_page |
135 |
op_container_end_page |
144 |
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1766361335022485504 |