Isolation of denitrifying bacteria from hydrocarbon-contaminated Antarctic soil
In this study, we report the isolation of denitrifiers from hydrocarbon-contaminated Antarctic soils. Seventy-two isolates were obtained from soils that had received a fertilizer treatment to stimulate hydrocarbon degradation. All isolates, except one, belonged to the genus Pseudomonas. The one exce...
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ftunivtasecite:oai:ecite.utas.edu.au:35973 2023-05-15T14:03:55+02:00 Isolation of denitrifying bacteria from hydrocarbon-contaminated Antarctic soil Powell, S Ma, WK Siciliano, SD 2006 https://doi.org/10.1007/s00300-006-0161-2 http://ecite.utas.edu.au/35973 en eng Springer-Verlag http://dx.doi.org/10.1007/s00300-006-0161-2 Powell, S and Ma, WK and Siciliano, SD, Isolation of denitrifying bacteria from hydrocarbon-contaminated Antarctic soil, Polar Biology, 30, (1) pp. 69-74. ISSN 0722-4060 (2006) [Refereed Article] http://ecite.utas.edu.au/35973 Biological Sciences Microbiology Bacteriology Refereed Article PeerReviewed 2006 ftunivtasecite https://doi.org/10.1007/s00300-006-0161-2 2019-12-13T21:14:21Z In this study, we report the isolation of denitrifiers from hydrocarbon-contaminated Antarctic soils. Seventy-two isolates were obtained from soils that had received a fertilizer treatment to stimulate hydrocarbon degradation. All isolates, except one, belonged to the genus Pseudomonas. The one exception was a member of the Microbacteriaceae, which was also, coincidentally, the only isolate negative for the nirS gene. The diversity of the 16S rRNA and nosZ genes was assessed by denaturing gradient gel electrophoresis and sequencing. There was a slight correlation between the 16S rRNA and nosZ operational taxonomic units. Surprisingly, many isolates contained nosZ on plasmids and, to the best of our knowledge, this is the first report of nosZ being extra-chromosomally present in Pseudomonas spp. 2006 Springer-Verlag. Article in Journal/Newspaper Antarc* Antarctic Polar Biology eCite UTAS (University of Tasmania) Antarctic Polar Biology 30 1 69 74 |
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Open Polar |
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eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Biological Sciences Microbiology Bacteriology |
spellingShingle |
Biological Sciences Microbiology Bacteriology Powell, S Ma, WK Siciliano, SD Isolation of denitrifying bacteria from hydrocarbon-contaminated Antarctic soil |
topic_facet |
Biological Sciences Microbiology Bacteriology |
description |
In this study, we report the isolation of denitrifiers from hydrocarbon-contaminated Antarctic soils. Seventy-two isolates were obtained from soils that had received a fertilizer treatment to stimulate hydrocarbon degradation. All isolates, except one, belonged to the genus Pseudomonas. The one exception was a member of the Microbacteriaceae, which was also, coincidentally, the only isolate negative for the nirS gene. The diversity of the 16S rRNA and nosZ genes was assessed by denaturing gradient gel electrophoresis and sequencing. There was a slight correlation between the 16S rRNA and nosZ operational taxonomic units. Surprisingly, many isolates contained nosZ on plasmids and, to the best of our knowledge, this is the first report of nosZ being extra-chromosomally present in Pseudomonas spp. 2006 Springer-Verlag. |
format |
Article in Journal/Newspaper |
author |
Powell, S Ma, WK Siciliano, SD |
author_facet |
Powell, S Ma, WK Siciliano, SD |
author_sort |
Powell, S |
title |
Isolation of denitrifying bacteria from hydrocarbon-contaminated Antarctic soil |
title_short |
Isolation of denitrifying bacteria from hydrocarbon-contaminated Antarctic soil |
title_full |
Isolation of denitrifying bacteria from hydrocarbon-contaminated Antarctic soil |
title_fullStr |
Isolation of denitrifying bacteria from hydrocarbon-contaminated Antarctic soil |
title_full_unstemmed |
Isolation of denitrifying bacteria from hydrocarbon-contaminated Antarctic soil |
title_sort |
isolation of denitrifying bacteria from hydrocarbon-contaminated antarctic soil |
publisher |
Springer-Verlag |
publishDate |
2006 |
url |
https://doi.org/10.1007/s00300-006-0161-2 http://ecite.utas.edu.au/35973 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Polar Biology |
genre_facet |
Antarc* Antarctic Polar Biology |
op_relation |
http://dx.doi.org/10.1007/s00300-006-0161-2 Powell, S and Ma, WK and Siciliano, SD, Isolation of denitrifying bacteria from hydrocarbon-contaminated Antarctic soil, Polar Biology, 30, (1) pp. 69-74. ISSN 0722-4060 (2006) [Refereed Article] http://ecite.utas.edu.au/35973 |
op_doi |
https://doi.org/10.1007/s00300-006-0161-2 |
container_title |
Polar Biology |
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30 |
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1 |
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69 |
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74 |
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1766274790310543360 |