Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon
In order to determine if elevated temperature during vitellogenesis had a detrimental effect on hepatic estrogen receptors of Atlantic salmon (Salmo salar), 3H-estradiol saturation binding analysis, using one- and two-site binding models, was carried out on extracts of hepatic cytosols from fish hel...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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Language: | English |
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Elselvier Inc.
2005
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Online Access: | https://doi.org/10.1016/j.cbpb.2005.02.002 http://www.ncbi.nlm.nih.gov/pubmed/15792603 http://ecite.utas.edu.au/32835 |
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ftunivtasecite:oai:ecite.utas.edu.au:32835 2023-05-15T15:31:25+02:00 Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon Watts, M Pankhurst, NW King, H Geraghty, DP 2005 https://doi.org/10.1016/j.cbpb.2005.02.002 http://www.ncbi.nlm.nih.gov/pubmed/15792603 http://ecite.utas.edu.au/32835 en eng Elselvier Inc. http://dx.doi.org/10.1016/j.cbpb.2005.02.002 Watts, M and Pankhurst, NW and King, H and Geraghty, DP, Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon, Comparative Biochemistry and Physiology. Part A: Molecular & Integrated Physiology, 140, (3) pp. 377-383. ISSN 1095-6433 (2005) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/15792603 http://ecite.utas.edu.au/32835 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Refereed Article PeerReviewed 2005 ftunivtasecite https://doi.org/10.1016/j.cbpb.2005.02.002 2019-12-13T21:12:00Z In order to determine if elevated temperature during vitellogenesis had a detrimental effect on hepatic estrogen receptors of Atlantic salmon (Salmo salar), 3H-estradiol saturation binding analysis, using one- and two-site binding models, was carried out on extracts of hepatic cytosols from fish held at 14, 18 or 22C over the austral period of peak vitellogenesis (February to April). With one-site binding analysis, no temperature related difference in either receptor affinity (Kd) or number (B max) was found at each sampling point, but there was an apparent decrease in both affinity and number at each temperature over the period of the study. However, some analyses, notably at 22C during February, were best described using a two-site binding model. At this temperature and time, there was a clear separation of binding affinity into high and low components (K d=0.67S.E. 0.05 and 20S.E. 5.6 nM, respectively) (n=4), which suggests that February was a critical time of temperature related hepatic sensitivity to estrogen. These results support those of other studies where we found that February was also a sensitive time with respect to temperature impairment of in vitro follicular estrogen synthesis, and the greatest period of in vivo temperature sensitivity. 2005 Elsevier Inc. All rights reserved. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Austral Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 140 3 377 383 |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
spellingShingle |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Watts, M Pankhurst, NW King, H Geraghty, DP Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
description |
In order to determine if elevated temperature during vitellogenesis had a detrimental effect on hepatic estrogen receptors of Atlantic salmon (Salmo salar), 3H-estradiol saturation binding analysis, using one- and two-site binding models, was carried out on extracts of hepatic cytosols from fish held at 14, 18 or 22C over the austral period of peak vitellogenesis (February to April). With one-site binding analysis, no temperature related difference in either receptor affinity (Kd) or number (B max) was found at each sampling point, but there was an apparent decrease in both affinity and number at each temperature over the period of the study. However, some analyses, notably at 22C during February, were best described using a two-site binding model. At this temperature and time, there was a clear separation of binding affinity into high and low components (K d=0.67S.E. 0.05 and 20S.E. 5.6 nM, respectively) (n=4), which suggests that February was a critical time of temperature related hepatic sensitivity to estrogen. These results support those of other studies where we found that February was also a sensitive time with respect to temperature impairment of in vitro follicular estrogen synthesis, and the greatest period of in vivo temperature sensitivity. 2005 Elsevier Inc. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Watts, M Pankhurst, NW King, H Geraghty, DP |
author_facet |
Watts, M Pankhurst, NW King, H Geraghty, DP |
author_sort |
Watts, M |
title |
Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon |
title_short |
Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon |
title_full |
Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon |
title_fullStr |
Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon |
title_full_unstemmed |
Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon |
title_sort |
differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of atlantic salmon |
publisher |
Elselvier Inc. |
publishDate |
2005 |
url |
https://doi.org/10.1016/j.cbpb.2005.02.002 http://www.ncbi.nlm.nih.gov/pubmed/15792603 http://ecite.utas.edu.au/32835 |
geographic |
Austral |
geographic_facet |
Austral |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://dx.doi.org/10.1016/j.cbpb.2005.02.002 Watts, M and Pankhurst, NW and King, H and Geraghty, DP, Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon, Comparative Biochemistry and Physiology. Part A: Molecular & Integrated Physiology, 140, (3) pp. 377-383. ISSN 1095-6433 (2005) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/15792603 http://ecite.utas.edu.au/32835 |
op_doi |
https://doi.org/10.1016/j.cbpb.2005.02.002 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
140 |
container_issue |
3 |
container_start_page |
377 |
op_container_end_page |
383 |
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1766361923206512640 |