The induction of laboratory-based amoebic gill disease revisited
Previous work in our laboratory defined a method of inducing laboratory-based amoebic gill disease (AGD) in Atlantic salmon, Salmo salar L. Gills of AGD-affected fish were scraped and the debris placed into fish-holding systems, eliciting AGD in nave Atlantic salmon. While this method is consistentl...
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Online Access: | https://doi.org/10.1111/j.1365-2761.2004.00561.x http://www.ncbi.nlm.nih.gov/pubmed/15291786 http://ecite.utas.edu.au/31383 |
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ftunivtasecite:oai:ecite.utas.edu.au:31383 2023-05-15T15:30:09+02:00 The induction of laboratory-based amoebic gill disease revisited Morrison, RN Crosbie, PBB Nowak, BF 2004 https://doi.org/10.1111/j.1365-2761.2004.00561.x http://www.ncbi.nlm.nih.gov/pubmed/15291786 http://ecite.utas.edu.au/31383 en eng Blackwell Publishing Ltd http://dx.doi.org/10.1111/j.1365-2761.2004.00561.x Morrison, RN and Crosbie, PBB and Nowak, BF, The induction of laboratory-based amoebic gill disease revisited, Journal of Fish Diseases, 27, (8) pp. 445-449. ISSN 0140-7775 (2004) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/15291786 http://ecite.utas.edu.au/31383 Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases Refereed Article PeerReviewed 2004 ftunivtasecite https://doi.org/10.1111/j.1365-2761.2004.00561.x 2019-12-13T21:11:02Z Previous work in our laboratory defined a method of inducing laboratory-based amoebic gill disease (AGD) in Atlantic salmon, Salmo salar L. Gills of AGD-affected fish were scraped and the debris placed into fish-holding systems, eliciting AGD in nave Atlantic salmon. While this method is consistently successful in inducing AGD, variability in the kinetics and severity of infections has been observed. It is believed that the infections are influenced by inherently variable viability of postharvest amoeba trophozoites. Here, a new method of experimental induction of AGD is presented that redefines the infection model including the minimum infective dose. Amoebae were partially purified from the gills of AGD-affected Atlantic salmon. Trophozoites were characterized by light microscopy and immunocytochemistry and designated Neoparamoeba sp., possibly Neoparamoeba pemaquidensis. Cells were placed into experimental infection systems ranging in concentration from 0 to 500 cells L-1. AGD was detected by gross and histological examination in fish held in all systems inoculated with amoebae. The number of gross and histological AGD lesions per gill was proportional to the inoculating concentration of amoebae indicating that the severity of disease is a function of amoeba density in the water column. The implications of these observations are discussed in the context of the existing AGD literature base as well as Atlantic salmon farming in south-eastern Tasmania. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Journal of Fish Diseases 27 8 445 449 |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases |
spellingShingle |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases Morrison, RN Crosbie, PBB Nowak, BF The induction of laboratory-based amoebic gill disease revisited |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases |
description |
Previous work in our laboratory defined a method of inducing laboratory-based amoebic gill disease (AGD) in Atlantic salmon, Salmo salar L. Gills of AGD-affected fish were scraped and the debris placed into fish-holding systems, eliciting AGD in nave Atlantic salmon. While this method is consistently successful in inducing AGD, variability in the kinetics and severity of infections has been observed. It is believed that the infections are influenced by inherently variable viability of postharvest amoeba trophozoites. Here, a new method of experimental induction of AGD is presented that redefines the infection model including the minimum infective dose. Amoebae were partially purified from the gills of AGD-affected Atlantic salmon. Trophozoites were characterized by light microscopy and immunocytochemistry and designated Neoparamoeba sp., possibly Neoparamoeba pemaquidensis. Cells were placed into experimental infection systems ranging in concentration from 0 to 500 cells L-1. AGD was detected by gross and histological examination in fish held in all systems inoculated with amoebae. The number of gross and histological AGD lesions per gill was proportional to the inoculating concentration of amoebae indicating that the severity of disease is a function of amoeba density in the water column. The implications of these observations are discussed in the context of the existing AGD literature base as well as Atlantic salmon farming in south-eastern Tasmania. |
format |
Article in Journal/Newspaper |
author |
Morrison, RN Crosbie, PBB Nowak, BF |
author_facet |
Morrison, RN Crosbie, PBB Nowak, BF |
author_sort |
Morrison, RN |
title |
The induction of laboratory-based amoebic gill disease revisited |
title_short |
The induction of laboratory-based amoebic gill disease revisited |
title_full |
The induction of laboratory-based amoebic gill disease revisited |
title_fullStr |
The induction of laboratory-based amoebic gill disease revisited |
title_full_unstemmed |
The induction of laboratory-based amoebic gill disease revisited |
title_sort |
induction of laboratory-based amoebic gill disease revisited |
publisher |
Blackwell Publishing Ltd |
publishDate |
2004 |
url |
https://doi.org/10.1111/j.1365-2761.2004.00561.x http://www.ncbi.nlm.nih.gov/pubmed/15291786 http://ecite.utas.edu.au/31383 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://dx.doi.org/10.1111/j.1365-2761.2004.00561.x Morrison, RN and Crosbie, PBB and Nowak, BF, The induction of laboratory-based amoebic gill disease revisited, Journal of Fish Diseases, 27, (8) pp. 445-449. ISSN 0140-7775 (2004) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/15291786 http://ecite.utas.edu.au/31383 |
op_doi |
https://doi.org/10.1111/j.1365-2761.2004.00561.x |
container_title |
Journal of Fish Diseases |
container_volume |
27 |
container_issue |
8 |
container_start_page |
445 |
op_container_end_page |
449 |
_version_ |
1766360590116192256 |