In vitro interactions between Neoparamoeba sp and Atlantic salmon epithelial cells
Neoparamoeba sp., including the putative aetiological agent of amoebic gill disease in cultured fish (N. pemaquidensis), were incubated in vitro with an Atlantic salmon gill epithelium (RGE-2) cell line. Proliferation by the amoeba population was dependent upon culture osmolarity; no growth occurred...
Published in: | Journal of Fish Diseases |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
ENGLAND
2004
|
Subjects: | |
Online Access: | https://doi.org/10.1111/j.1365-2761.2004.00550.x http://www.ncbi.nlm.nih.gov/pubmed/15189374 http://ecite.utas.edu.au/31209 |
id |
ftunivtasecite:oai:ecite.utas.edu.au:31209 |
---|---|
record_format |
openpolar |
spelling |
ftunivtasecite:oai:ecite.utas.edu.au:31209 2023-05-15T15:31:10+02:00 In vitro interactions between Neoparamoeba sp and Atlantic salmon epithelial cells Butler, R Nowak, BF 2004 https://doi.org/10.1111/j.1365-2761.2004.00550.x http://www.ncbi.nlm.nih.gov/pubmed/15189374 http://ecite.utas.edu.au/31209 en eng ENGLAND http://dx.doi.org/10.1111/j.1365-2761.2004.00550.x Butler, R and Nowak, BF, In vitro interactions between Neoparamoeba sp and Atlantic salmon epithelial cells, Journal of Fish Diseases, 27, (6) pp. 343-349. ISSN 0140-7775 (2004) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/15189374 http://ecite.utas.edu.au/31209 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Refereed Article PeerReviewed 2004 ftunivtasecite https://doi.org/10.1111/j.1365-2761.2004.00550.x 2019-12-13T21:10:50Z Neoparamoeba sp., including the putative aetiological agent of amoebic gill disease in cultured fish (N. pemaquidensis), were incubated in vitro with an Atlantic salmon gill epithelium (RGE-2) cell line. Proliferation by the amoeba population was dependent upon culture osmolarity; no growth occurred at 330 mM kg-1 but a sixfold increase was observed at 1000 mM kg-1. At 780 mM kg-1 there was a fourfold increase in the amoeba population but a concurrent decrease in RGE-2 cell density that was significantly greater than that caused by the high culture osmolarity alone. This apparent cytopathic effect (CPE) developed rapidly and resulted in complete cytolysis of the monolayer in 5 days. CPE occurred in multiple foci and presented as cell vacuolation, rounding and clumping, and the rapid clearance of large areas of the cell monolayer. The possibility that CPE is because of the presence of Neoparamoeba sp. derived cytolytic products is discussed in the context of the pathology of the disease in vivo and the occurrence of secreted cytopathogenic compounds in other amoeba species. Article in Journal/Newspaper Atlantic salmon eCite UTAS (University of Tasmania) Journal of Fish Diseases 27 6 343 349 |
institution |
Open Polar |
collection |
eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
spellingShingle |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Butler, R Nowak, BF In vitro interactions between Neoparamoeba sp and Atlantic salmon epithelial cells |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
description |
Neoparamoeba sp., including the putative aetiological agent of amoebic gill disease in cultured fish (N. pemaquidensis), were incubated in vitro with an Atlantic salmon gill epithelium (RGE-2) cell line. Proliferation by the amoeba population was dependent upon culture osmolarity; no growth occurred at 330 mM kg-1 but a sixfold increase was observed at 1000 mM kg-1. At 780 mM kg-1 there was a fourfold increase in the amoeba population but a concurrent decrease in RGE-2 cell density that was significantly greater than that caused by the high culture osmolarity alone. This apparent cytopathic effect (CPE) developed rapidly and resulted in complete cytolysis of the monolayer in 5 days. CPE occurred in multiple foci and presented as cell vacuolation, rounding and clumping, and the rapid clearance of large areas of the cell monolayer. The possibility that CPE is because of the presence of Neoparamoeba sp. derived cytolytic products is discussed in the context of the pathology of the disease in vivo and the occurrence of secreted cytopathogenic compounds in other amoeba species. |
format |
Article in Journal/Newspaper |
author |
Butler, R Nowak, BF |
author_facet |
Butler, R Nowak, BF |
author_sort |
Butler, R |
title |
In vitro interactions between Neoparamoeba sp and Atlantic salmon epithelial cells |
title_short |
In vitro interactions between Neoparamoeba sp and Atlantic salmon epithelial cells |
title_full |
In vitro interactions between Neoparamoeba sp and Atlantic salmon epithelial cells |
title_fullStr |
In vitro interactions between Neoparamoeba sp and Atlantic salmon epithelial cells |
title_full_unstemmed |
In vitro interactions between Neoparamoeba sp and Atlantic salmon epithelial cells |
title_sort |
in vitro interactions between neoparamoeba sp and atlantic salmon epithelial cells |
publisher |
ENGLAND |
publishDate |
2004 |
url |
https://doi.org/10.1111/j.1365-2761.2004.00550.x http://www.ncbi.nlm.nih.gov/pubmed/15189374 http://ecite.utas.edu.au/31209 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://dx.doi.org/10.1111/j.1365-2761.2004.00550.x Butler, R and Nowak, BF, In vitro interactions between Neoparamoeba sp and Atlantic salmon epithelial cells, Journal of Fish Diseases, 27, (6) pp. 343-349. ISSN 0140-7775 (2004) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/15189374 http://ecite.utas.edu.au/31209 |
op_doi |
https://doi.org/10.1111/j.1365-2761.2004.00550.x |
container_title |
Journal of Fish Diseases |
container_volume |
27 |
container_issue |
6 |
container_start_page |
343 |
op_container_end_page |
349 |
_version_ |
1766361656796905472 |