Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community
A field experiment investigating the effect of oil contamination on benthic microbial communities was conducted near Casey Station, East Antarctica. Defaunated sediment was treated with a mixture of Special Antarctic Blend diesel and lubricating oil and deployed in three different bays for eleven we...
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2005
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Online Access: | https://doi.org/10.1017/S0954102005002786 http://ecite.utas.edu.au/29356 |
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ftunivtasecite:oai:ecite.utas.edu.au:29356 2023-05-15T14:03:54+02:00 Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community Powell, S Riddle, MJ Snape, I Stark, JS 2005 https://doi.org/10.1017/S0954102005002786 http://ecite.utas.edu.au/29356 en eng Cambridge University Press http://dx.doi.org/10.1017/S0954102005002786 Powell, S and Riddle, MJ and Snape, I and Stark, JS, Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community, Antarctic Science, 17, (3) pp. 353-360. ISSN 0954-1020 (2005) [Refereed Article] http://ecite.utas.edu.au/29356 Biological Sciences Microbiology Microbial Ecology Refereed Article PeerReviewed 2005 ftunivtasecite https://doi.org/10.1017/S0954102005002786 2019-12-13T21:09:33Z A field experiment investigating the effect of oil contamination on benthic microbial communities was conducted near Casey Station, East Antarctica. Defaunated sediment was treated with a mixture of Special Antarctic Blend diesel and lubricating oil and deployed in three different bays for eleven weeks. A molecular fingerprinting technique, denaturing gradient gel electrophoresis (DGGE), was used to investigate the microbial community structure. The variation between replicate samples within treatment groups indicates that the benthic microbial populations are very diverse and evenly distributed. Comparisons to determine the significance of both deployment location and hydrocarbon treatment showed that the greatest effect was from a combination of location and treatment. Detailed analysis suggests that subtle differences may be obscured by variability introduced by PCR and gel stages in DGGE, undermining this experimental approach. It is concluded that both location and hydrocarbon contamination influenced the development of the microbial communities but that the effect of hydrocarbon treatment varied with location. This has important implications for the design of future experiments on the effect of hydrocarbons on benthic communities, especially if it is intended to generalize the conclusions drawn from site specific studies. Antarctic Science Ltd. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Antarctica East Antarctica eCite UTAS (University of Tasmania) Antarctic Casey Station ENVELOPE(110.528,110.528,-66.282,-66.282) East Antarctica Antarctic Science 17 3 353 360 |
institution |
Open Polar |
collection |
eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Biological Sciences Microbiology Microbial Ecology |
spellingShingle |
Biological Sciences Microbiology Microbial Ecology Powell, S Riddle, MJ Snape, I Stark, JS Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community |
topic_facet |
Biological Sciences Microbiology Microbial Ecology |
description |
A field experiment investigating the effect of oil contamination on benthic microbial communities was conducted near Casey Station, East Antarctica. Defaunated sediment was treated with a mixture of Special Antarctic Blend diesel and lubricating oil and deployed in three different bays for eleven weeks. A molecular fingerprinting technique, denaturing gradient gel electrophoresis (DGGE), was used to investigate the microbial community structure. The variation between replicate samples within treatment groups indicates that the benthic microbial populations are very diverse and evenly distributed. Comparisons to determine the significance of both deployment location and hydrocarbon treatment showed that the greatest effect was from a combination of location and treatment. Detailed analysis suggests that subtle differences may be obscured by variability introduced by PCR and gel stages in DGGE, undermining this experimental approach. It is concluded that both location and hydrocarbon contamination influenced the development of the microbial communities but that the effect of hydrocarbon treatment varied with location. This has important implications for the design of future experiments on the effect of hydrocarbons on benthic communities, especially if it is intended to generalize the conclusions drawn from site specific studies. Antarctic Science Ltd. |
format |
Article in Journal/Newspaper |
author |
Powell, S Riddle, MJ Snape, I Stark, JS |
author_facet |
Powell, S Riddle, MJ Snape, I Stark, JS |
author_sort |
Powell, S |
title |
Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community |
title_short |
Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community |
title_full |
Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community |
title_fullStr |
Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community |
title_full_unstemmed |
Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community |
title_sort |
location and dgge methodology can influence interpretation of field experimental studies on the response to hydrocarbons by antarctic benthic microbial community |
publisher |
Cambridge University Press |
publishDate |
2005 |
url |
https://doi.org/10.1017/S0954102005002786 http://ecite.utas.edu.au/29356 |
long_lat |
ENVELOPE(110.528,110.528,-66.282,-66.282) |
geographic |
Antarctic Casey Station East Antarctica |
geographic_facet |
Antarctic Casey Station East Antarctica |
genre |
Antarc* Antarctic Antarctic Science Antarctica East Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Science Antarctica East Antarctica |
op_relation |
http://dx.doi.org/10.1017/S0954102005002786 Powell, S and Riddle, MJ and Snape, I and Stark, JS, Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community, Antarctic Science, 17, (3) pp. 353-360. ISSN 0954-1020 (2005) [Refereed Article] http://ecite.utas.edu.au/29356 |
op_doi |
https://doi.org/10.1017/S0954102005002786 |
container_title |
Antarctic Science |
container_volume |
17 |
container_issue |
3 |
container_start_page |
353 |
op_container_end_page |
360 |
_version_ |
1766274773173665792 |