Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean
The abundance, spatial distribution and diversity of class Flavobacteria were investigated in the Southern Ocean euphotic zone across a latitudinal transect and in the ice pack off Eastern Antarctica. Surface seawater samples filter-fractionated into 0.8 m particulate and 0.2 m planktonic fractions...
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Online Access: | https://doi.org/10.1016/j.femsec.2004.09.001 http://www.ncbi.nlm.nih.gov/pubmed/16329875 http://ecite.utas.edu.au/29235 |
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ftunivtasecite:oai:ecite.utas.edu.au:29235 2023-05-15T14:03:54+02:00 Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean Abell, GC Bowman, JP 2005 https://doi.org/10.1016/j.femsec.2004.09.001 http://www.ncbi.nlm.nih.gov/pubmed/16329875 http://ecite.utas.edu.au/29235 en eng Elsevier Science BV http://dx.doi.org/10.1016/j.femsec.2004.09.001 Abell, GC and Bowman, JP, Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean, FEMS Microbiology Ecology, 51, (2) pp. 265-277. ISSN 0168-6496 (2005) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/16329875 http://ecite.utas.edu.au/29235 Biological Sciences Microbiology Microbial Ecology Refereed Article PeerReviewed 2005 ftunivtasecite https://doi.org/10.1016/j.femsec.2004.09.001 2019-12-13T21:09:27Z The abundance, spatial distribution and diversity of class Flavobacteria were investigated in the Southern Ocean euphotic zone across a latitudinal transect and in the ice pack off Eastern Antarctica. Surface seawater samples filter-fractionated into 0.8 m particulate and 0.2 m planktonic fractions were investigated with different molecular techniques. The abundance of particle-associated Flavobacteria, ascertained with real-time PCR and DGGE band analysis using Flavobacteria-specific primers, was found to be significantly higher in Polar Front Zone (PFZ) and Antarctic Zone (AZ) water samples than in nutrient limited Temperate Zone (TZ) and Sub-Antarctic Zone (SAZ) waters. Abundance of particle-associated Flavobacteria correlated positively with seawater chlorophyll a and nutrient concentrations, suggesting that increased Flavobacteria abundance may relate to enhanced primary production in the PFZ and AZ. This is supported by comparison of DGGE profiles that demonstrated significant differences in the total Flavobacteria community structure and 16S rRNA gene diversity between samples from the PFZ and AZ and those from TZ and SAZ. Sequence analysis revealed a broad diversity amongst class Flavobacteria in the Southern Ocean with several Flavobacteria clades detected in PFZ and AZ waters not detected in TZ and SAZ waters that putatively represent psychrophilic taxa. Sequence data included a large, so far uncultivated, cosmopolitan phylogenetic clade ("DE cluster 2") that is distributed throughout the Southern Ocean. 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. Article in Journal/Newspaper Antarc* Antarctic Antarctica Southern Ocean eCite UTAS (University of Tasmania) Antarctic Southern Ocean FEMS Microbiology Ecology 51 2 265 277 |
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Open Polar |
collection |
eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Biological Sciences Microbiology Microbial Ecology |
spellingShingle |
Biological Sciences Microbiology Microbial Ecology Abell, GC Bowman, JP Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean |
topic_facet |
Biological Sciences Microbiology Microbial Ecology |
description |
The abundance, spatial distribution and diversity of class Flavobacteria were investigated in the Southern Ocean euphotic zone across a latitudinal transect and in the ice pack off Eastern Antarctica. Surface seawater samples filter-fractionated into 0.8 m particulate and 0.2 m planktonic fractions were investigated with different molecular techniques. The abundance of particle-associated Flavobacteria, ascertained with real-time PCR and DGGE band analysis using Flavobacteria-specific primers, was found to be significantly higher in Polar Front Zone (PFZ) and Antarctic Zone (AZ) water samples than in nutrient limited Temperate Zone (TZ) and Sub-Antarctic Zone (SAZ) waters. Abundance of particle-associated Flavobacteria correlated positively with seawater chlorophyll a and nutrient concentrations, suggesting that increased Flavobacteria abundance may relate to enhanced primary production in the PFZ and AZ. This is supported by comparison of DGGE profiles that demonstrated significant differences in the total Flavobacteria community structure and 16S rRNA gene diversity between samples from the PFZ and AZ and those from TZ and SAZ. Sequence analysis revealed a broad diversity amongst class Flavobacteria in the Southern Ocean with several Flavobacteria clades detected in PFZ and AZ waters not detected in TZ and SAZ waters that putatively represent psychrophilic taxa. Sequence data included a large, so far uncultivated, cosmopolitan phylogenetic clade ("DE cluster 2") that is distributed throughout the Southern Ocean. 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Abell, GC Bowman, JP |
author_facet |
Abell, GC Bowman, JP |
author_sort |
Abell, GC |
title |
Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean |
title_short |
Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean |
title_full |
Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean |
title_fullStr |
Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean |
title_full_unstemmed |
Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean |
title_sort |
ecological and biogeographic relationships of class flavobacteria in the southern ocean |
publisher |
Elsevier Science BV |
publishDate |
2005 |
url |
https://doi.org/10.1016/j.femsec.2004.09.001 http://www.ncbi.nlm.nih.gov/pubmed/16329875 http://ecite.utas.edu.au/29235 |
geographic |
Antarctic Southern Ocean |
geographic_facet |
Antarctic Southern Ocean |
genre |
Antarc* Antarctic Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Southern Ocean |
op_relation |
http://dx.doi.org/10.1016/j.femsec.2004.09.001 Abell, GC and Bowman, JP, Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean, FEMS Microbiology Ecology, 51, (2) pp. 265-277. ISSN 0168-6496 (2005) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/16329875 http://ecite.utas.edu.au/29235 |
op_doi |
https://doi.org/10.1016/j.femsec.2004.09.001 |
container_title |
FEMS Microbiology Ecology |
container_volume |
51 |
container_issue |
2 |
container_start_page |
265 |
op_container_end_page |
277 |
_version_ |
1766274772301250560 |