Relationship of nitrogen isotope fractionation to phytoplankton size and iron availability during the Southern Ocean Iron Release Experiment (SOIREE)

• Published in: Limnology and Oceanography
• Main Authors: Karsh, K, Trull, T, Lourey, MJ, Sigman, DM
• Format: Article in Journal/Newspaper
• Language: English
• Published: American Society of Limnology and Oceanography 2003
• Subjects: Physical Sciences; Optical Physics; Lasers and Quantum Electronics; Southern Ocean
• Online Access: https://doi.org/10.4319/lo.2003.48.3.1058; http://ecite.utas.edu.au/29155

The 15N composition of sediments has been used as a proxy for nitrate utilization in surface waters to assess the role of Southern Ocean export production in glacial/interglacial changes in atmospheric CO2 concentration. Interpretation has relied on a temporally constant isotope effect () associated with uptake and assimilation of nitrate by phytoplankton. To investigate the reliability of this approach, we examined the relationships between the 15N compositions of dissolved nitrate, bulk and size-fractionated (200, 70, 20, 5, 1 m) suspended particulate organic nitrogen (PON), and sinking particles obtained from sediment traps during the Southern Ocean iron release experiment (SOIREE). We found variations in phytoplankton nitrogen isotopic compositions with both cell size and iron availability. 15NPON increased by <2 with increasing size, both within and outside the iron-enriched patch. In comparison to unfertilized waters, 15NPON within the iron-fertilized patch was a further 3-4 higher in those size fractions dominated by large diatoms (20-70, 70-200 m). We speculate that this iron response might result from (1) variation in of nitrate utilization or (2) an iron-stimulated shift from ammonium-based to nitrate-based production. Comparing the 15N of the large diatom-dominated size fractions to the 15N of nitrate suggests relatively low values of 4-5, in contrast to estimated values of 7-10 from seasonal nitrate depletion and export production. This suggests that higher glacial 15N in Southern Ocean sediments could, in part, reflect increases in iron availability, dominant cell size, and possibly growth rates, and these effects must be considered in any quantitative scaling of 15N variations, including those of diatom-bound 15N, to the extent of nitrate utilization.