On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with Elution Using Transient-Isotachophoretic Gradients. 3. Implementation and Method Development
A solid-phase extraction method based on an ionexchange retention mechanism has been used for in-line preconcentration of inorganic anions prior to their separation by capillary electrophoresis (CE). A single capillary containing a preconcentration and a separation zone has been used in a commercial...
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ftunivtasecite:oai:ecite.utas.edu.au:26114 2023-05-15T13:59:07+02:00 On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with Elution Using Transient-Isotachophoretic Gradients. 3. Implementation and Method Development Breadmore, MC Palmer, AS Curran, MAJ Macka, M Avdalovic, N Haddad, PR 2002 https://doi.org/10.1021/ac011217u http://www.ncbi.nlm.nih.gov/pubmed/12033314 http://ecite.utas.edu.au/26114 en eng Americal Chemical Society http://dx.doi.org/10.1021/ac011217u Breadmore, MC and Palmer, AS and Curran, MAJ and Macka, M and Avdalovic, N and Haddad, PR, On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with Elution Using Transient-Isotachophoretic Gradients. 3. Implementation and Method Development, Analytical Chemistry, 74, (9) pp. 2112-2118. ISSN 0003-2700 (2002) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/12033314 http://ecite.utas.edu.au/26114 Chemical Sciences Analytical Chemistry Separation Science Refereed Article PeerReviewed 2002 ftunivtasecite https://doi.org/10.1021/ac011217u 2019-12-13T21:07:04Z A solid-phase extraction method based on an ionexchange retention mechanism has been used for in-line preconcentration of inorganic anions prior to their separation by capillary electrophoresis (CE). A single capillary containing a preconcentration and a separation zone has been used in a commercial CE instrument without instrumental modification. Analyte anions were retained on a preconcentration zone comprising an adsorbed layer of cationic latex particles, while separation was achieved in a separation zone comprising fused silica modified by adsorption of a cationic polymer. Elution of the adsorbed analytes was achieved using an eluotropic gradient formed by a transient isotachophoretic boundary between a fluoride electrolyte and a naphthalenedisulfonate electrolyte. Optimization of the electrolyte concentrations, sample injection times, and back-flushing times allowed the successful separation of sub-ppb levels of inorganic anions using a 100-min injection at 2 bar pressure, introducing over 40 capillary volumes of sample. A method based on a 10-min injection allowed a 100-fold increase in sensitivity over conventional hydrodynamic injection for Br-, I-, NO3 -, CrO4 2-, and MoO4 2- with a total analysis time of 25 min. Detection limits were dependent on the injection time but were in the range 2.2-11.6 ppb for a 10-min injection time. This approach was used to determine NO3 - in Antarctic ice cores where the analysis could be performed using a sample volume 100 times less than that used for ion chromatography. Article in Journal/Newspaper Antarc* Antarctic eCite UTAS (University of Tasmania) Antarctic Analytical Chemistry 74 9 2112 2118 |
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Open Polar |
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eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Chemical Sciences Analytical Chemistry Separation Science |
spellingShingle |
Chemical Sciences Analytical Chemistry Separation Science Breadmore, MC Palmer, AS Curran, MAJ Macka, M Avdalovic, N Haddad, PR On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with Elution Using Transient-Isotachophoretic Gradients. 3. Implementation and Method Development |
topic_facet |
Chemical Sciences Analytical Chemistry Separation Science |
description |
A solid-phase extraction method based on an ionexchange retention mechanism has been used for in-line preconcentration of inorganic anions prior to their separation by capillary electrophoresis (CE). A single capillary containing a preconcentration and a separation zone has been used in a commercial CE instrument without instrumental modification. Analyte anions were retained on a preconcentration zone comprising an adsorbed layer of cationic latex particles, while separation was achieved in a separation zone comprising fused silica modified by adsorption of a cationic polymer. Elution of the adsorbed analytes was achieved using an eluotropic gradient formed by a transient isotachophoretic boundary between a fluoride electrolyte and a naphthalenedisulfonate electrolyte. Optimization of the electrolyte concentrations, sample injection times, and back-flushing times allowed the successful separation of sub-ppb levels of inorganic anions using a 100-min injection at 2 bar pressure, introducing over 40 capillary volumes of sample. A method based on a 10-min injection allowed a 100-fold increase in sensitivity over conventional hydrodynamic injection for Br-, I-, NO3 -, CrO4 2-, and MoO4 2- with a total analysis time of 25 min. Detection limits were dependent on the injection time but were in the range 2.2-11.6 ppb for a 10-min injection time. This approach was used to determine NO3 - in Antarctic ice cores where the analysis could be performed using a sample volume 100 times less than that used for ion chromatography. |
format |
Article in Journal/Newspaper |
author |
Breadmore, MC Palmer, AS Curran, MAJ Macka, M Avdalovic, N Haddad, PR |
author_facet |
Breadmore, MC Palmer, AS Curran, MAJ Macka, M Avdalovic, N Haddad, PR |
author_sort |
Breadmore, MC |
title |
On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with Elution Using Transient-Isotachophoretic Gradients. 3. Implementation and Method Development |
title_short |
On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with Elution Using Transient-Isotachophoretic Gradients. 3. Implementation and Method Development |
title_full |
On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with Elution Using Transient-Isotachophoretic Gradients. 3. Implementation and Method Development |
title_fullStr |
On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with Elution Using Transient-Isotachophoretic Gradients. 3. Implementation and Method Development |
title_full_unstemmed |
On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with Elution Using Transient-Isotachophoretic Gradients. 3. Implementation and Method Development |
title_sort |
on-column ion-exchange preconcentration of inorganic anions in open tubular capillary electrochromatography with elution using transient-isotachophoretic gradients. 3. implementation and method development |
publisher |
Americal Chemical Society |
publishDate |
2002 |
url |
https://doi.org/10.1021/ac011217u http://www.ncbi.nlm.nih.gov/pubmed/12033314 http://ecite.utas.edu.au/26114 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://dx.doi.org/10.1021/ac011217u Breadmore, MC and Palmer, AS and Curran, MAJ and Macka, M and Avdalovic, N and Haddad, PR, On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with Elution Using Transient-Isotachophoretic Gradients. 3. Implementation and Method Development, Analytical Chemistry, 74, (9) pp. 2112-2118. ISSN 0003-2700 (2002) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/12033314 http://ecite.utas.edu.au/26114 |
op_doi |
https://doi.org/10.1021/ac011217u |
container_title |
Analytical Chemistry |
container_volume |
74 |
container_issue |
9 |
container_start_page |
2112 |
op_container_end_page |
2118 |
_version_ |
1766267528077639680 |