Simple gill Smear Staining for Diagnosis of Amoebic Gill Disease
Amoebic gill disease (AGD) caused by a Paramoeba spp. is the major disease affecting cultured Atlantic salmon in Tasmania. Primary diagnosis of the disease on the farm relies on gross morphology, and it is then confirmed in the laboratory with an immunofluorescence antibody test (IFAT). In this stud...
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European Association of Fish Pathologists
1999
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ftunivtasecite:oai:ecite.utas.edu.au:16605 2023-05-15T15:32:25+02:00 Simple gill Smear Staining for Diagnosis of Amoebic Gill Disease Zilberg, D Nowak, BF Carson, J Wagner, T 1999 http://ecite.utas.edu.au/16605 en eng European Association of Fish Pathologists Zilberg, D and Nowak, BF and Carson, J and Wagner, T, Simple gill Smear Staining for Diagnosis of Amoebic Gill Disease, Bulletin of European Association of Fish Pathologists, 19, (5) pp. 186-189. ISSN 0108-0288 (1999) [Refereed Article] http://ecite.utas.edu.au/16605 Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases Refereed Article PeerReviewed 1999 ftunivtasecite 2019-12-13T21:00:00Z Amoebic gill disease (AGD) caused by a Paramoeba spp. is the major disease affecting cultured Atlantic salmon in Tasmania. Primary diagnosis of the disease on the farm relies on gross morphology, and it is then confirmed in the laboratory with an immunofluorescence antibody test (IFAT). In this study we evaluated the potential use of a rapid method for the detection of AGD using a Quick Dip* stain and compared it to the established IFAT. Quick Dip* staining revealed 96% agreement with IFAT in the determination whether a sample is positive or negative for AGD, with sensitivity and specificity values of 88.23% and 92.85%, respectively and a kappa value of 0.7628. Mean number of Paramoeba spp. per field of view positively correlated in the two methods (r=0.972), although it was significantly greater when IFAT was used. Quick Dip* stained Paramoeba spp. in a mucus smear is identified by its morphological features. It measures 15 to 20 m, appearing dark blue with a darker blue and purple-stained internal organelles. The study suggests that Quick Dip* can be used as a fast method for the diagnosis of AGD. Article in Journal/Newspaper Atlantic salmon eCite UTAS (University of Tasmania) |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases |
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Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases Zilberg, D Nowak, BF Carson, J Wagner, T Simple gill Smear Staining for Diagnosis of Amoebic Gill Disease |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases |
description |
Amoebic gill disease (AGD) caused by a Paramoeba spp. is the major disease affecting cultured Atlantic salmon in Tasmania. Primary diagnosis of the disease on the farm relies on gross morphology, and it is then confirmed in the laboratory with an immunofluorescence antibody test (IFAT). In this study we evaluated the potential use of a rapid method for the detection of AGD using a Quick Dip* stain and compared it to the established IFAT. Quick Dip* staining revealed 96% agreement with IFAT in the determination whether a sample is positive or negative for AGD, with sensitivity and specificity values of 88.23% and 92.85%, respectively and a kappa value of 0.7628. Mean number of Paramoeba spp. per field of view positively correlated in the two methods (r=0.972), although it was significantly greater when IFAT was used. Quick Dip* stained Paramoeba spp. in a mucus smear is identified by its morphological features. It measures 15 to 20 m, appearing dark blue with a darker blue and purple-stained internal organelles. The study suggests that Quick Dip* can be used as a fast method for the diagnosis of AGD. |
format |
Article in Journal/Newspaper |
author |
Zilberg, D Nowak, BF Carson, J Wagner, T |
author_facet |
Zilberg, D Nowak, BF Carson, J Wagner, T |
author_sort |
Zilberg, D |
title |
Simple gill Smear Staining for Diagnosis of Amoebic Gill Disease |
title_short |
Simple gill Smear Staining for Diagnosis of Amoebic Gill Disease |
title_full |
Simple gill Smear Staining for Diagnosis of Amoebic Gill Disease |
title_fullStr |
Simple gill Smear Staining for Diagnosis of Amoebic Gill Disease |
title_full_unstemmed |
Simple gill Smear Staining for Diagnosis of Amoebic Gill Disease |
title_sort |
simple gill smear staining for diagnosis of amoebic gill disease |
publisher |
European Association of Fish Pathologists |
publishDate |
1999 |
url |
http://ecite.utas.edu.au/16605 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Zilberg, D and Nowak, BF and Carson, J and Wagner, T, Simple gill Smear Staining for Diagnosis of Amoebic Gill Disease, Bulletin of European Association of Fish Pathologists, 19, (5) pp. 186-189. ISSN 0108-0288 (1999) [Refereed Article] http://ecite.utas.edu.au/16605 |
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1766362916923113472 |