Size-dependent resistance to amoebic gill disease in naive Atlantic salmon ( Salmo salar )
Amoebic gill disease , caused by the protozoan ectoparasite Neoparamoeba perurans , remains a significant threat to commercial Atlantic salmon aquaculture operations worldwide, despite partial control afforded by selective breeding and therapeutic intervention. Anecdotal reports from commercial prod...
Published in: | Fish & Shellfish Immunology |
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Academic Press Ltd Elsevier Science Ltd
2022
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Online Access: | https://doi.org/10.1016/j.fsi.2022.02.035 http://www.ncbi.nlm.nih.gov/pubmed/35189323 http://ecite.utas.edu.au/152037 |
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ftunivtasecite:oai:ecite.utas.edu.au:152037 2023-05-15T15:28:19+02:00 Size-dependent resistance to amoebic gill disease in naive Atlantic salmon ( Salmo salar ) Smith, AJ Adams, MB Crosbie, PBB Nowak, BF Bridle, AR 2022 https://doi.org/10.1016/j.fsi.2022.02.035 http://www.ncbi.nlm.nih.gov/pubmed/35189323 http://ecite.utas.edu.au/152037 en eng Academic Press Ltd Elsevier Science Ltd http://dx.doi.org/10.1016/j.fsi.2022.02.035 Smith, AJ and Adams, MB and Crosbie, PBB and Nowak, BF and Bridle, AR, Size-dependent resistance to amoebic gill disease in naive Atlantic salmon ( Salmo salar ), Fish and Shellfish Immunology, 122 pp. 437-445. ISSN 1050-4648 (2022) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/35189323 http://ecite.utas.edu.au/152037 Agricultural Veterinary and Food Sciences Fisheries sciences Aquaculture Refereed Article PeerReviewed 2022 ftunivtasecite https://doi.org/10.1016/j.fsi.2022.02.035 2022-11-21T23:17:12Z Amoebic gill disease , caused by the protozoan ectoparasite Neoparamoeba perurans , remains a significant threat to commercial Atlantic salmon aquaculture operations worldwide, despite partial control afforded by selective breeding and therapeutic intervention. Anecdotal reports from commercial producers suggest that historically, smaller Atlantic salmon smolts are more susceptible to AGD than larger smolts. Here, large (>350g) and small (<200g) commercially sourced, AGD-naive Atlantic salmon cohorts were experimentally exposed to 50 N. perurans trophozoites L −1 without intervention. Progression and severity of AGD in challenged cohorts was evaluated through gill pathology, using gill score and histological examination, and quantification of gill-associated amoebae burden using qPCR . To determine the potential basis for differences in AGD susceptibility between cohorts, transcriptome analysis was conducted using RNA extracted from whole gill arches. Overall, the large Atlantic salmon cohort had significantly lower gill parasite burdens and reduced AGD-related gross pathology compared to the small cohort. Relative gill load of N. perurans appeared to be proportional to gill score in both size classes, with larger smolts typically observed to have comparatively reduced parasite burdens at a given gill score. Moreover, comparison between gene expression profiles of large and small smolts highlighted upregulation of genes consistent with elevated immune activity in large smolts. Combined, the results presented here provide strong evidence of size-dependent resistance to AGD in AGD-naive Atlantic salmon. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Fish & Shellfish Immunology 122 437 445 |
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eCite UTAS (University of Tasmania) |
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language |
English |
topic |
Agricultural Veterinary and Food Sciences Fisheries sciences Aquaculture |
spellingShingle |
Agricultural Veterinary and Food Sciences Fisheries sciences Aquaculture Smith, AJ Adams, MB Crosbie, PBB Nowak, BF Bridle, AR Size-dependent resistance to amoebic gill disease in naive Atlantic salmon ( Salmo salar ) |
topic_facet |
Agricultural Veterinary and Food Sciences Fisheries sciences Aquaculture |
description |
Amoebic gill disease , caused by the protozoan ectoparasite Neoparamoeba perurans , remains a significant threat to commercial Atlantic salmon aquaculture operations worldwide, despite partial control afforded by selective breeding and therapeutic intervention. Anecdotal reports from commercial producers suggest that historically, smaller Atlantic salmon smolts are more susceptible to AGD than larger smolts. Here, large (>350g) and small (<200g) commercially sourced, AGD-naive Atlantic salmon cohorts were experimentally exposed to 50 N. perurans trophozoites L −1 without intervention. Progression and severity of AGD in challenged cohorts was evaluated through gill pathology, using gill score and histological examination, and quantification of gill-associated amoebae burden using qPCR . To determine the potential basis for differences in AGD susceptibility between cohorts, transcriptome analysis was conducted using RNA extracted from whole gill arches. Overall, the large Atlantic salmon cohort had significantly lower gill parasite burdens and reduced AGD-related gross pathology compared to the small cohort. Relative gill load of N. perurans appeared to be proportional to gill score in both size classes, with larger smolts typically observed to have comparatively reduced parasite burdens at a given gill score. Moreover, comparison between gene expression profiles of large and small smolts highlighted upregulation of genes consistent with elevated immune activity in large smolts. Combined, the results presented here provide strong evidence of size-dependent resistance to AGD in AGD-naive Atlantic salmon. |
format |
Article in Journal/Newspaper |
author |
Smith, AJ Adams, MB Crosbie, PBB Nowak, BF Bridle, AR |
author_facet |
Smith, AJ Adams, MB Crosbie, PBB Nowak, BF Bridle, AR |
author_sort |
Smith, AJ |
title |
Size-dependent resistance to amoebic gill disease in naive Atlantic salmon ( Salmo salar ) |
title_short |
Size-dependent resistance to amoebic gill disease in naive Atlantic salmon ( Salmo salar ) |
title_full |
Size-dependent resistance to amoebic gill disease in naive Atlantic salmon ( Salmo salar ) |
title_fullStr |
Size-dependent resistance to amoebic gill disease in naive Atlantic salmon ( Salmo salar ) |
title_full_unstemmed |
Size-dependent resistance to amoebic gill disease in naive Atlantic salmon ( Salmo salar ) |
title_sort |
size-dependent resistance to amoebic gill disease in naive atlantic salmon ( salmo salar ) |
publisher |
Academic Press Ltd Elsevier Science Ltd |
publishDate |
2022 |
url |
https://doi.org/10.1016/j.fsi.2022.02.035 http://www.ncbi.nlm.nih.gov/pubmed/35189323 http://ecite.utas.edu.au/152037 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://dx.doi.org/10.1016/j.fsi.2022.02.035 Smith, AJ and Adams, MB and Crosbie, PBB and Nowak, BF and Bridle, AR, Size-dependent resistance to amoebic gill disease in naive Atlantic salmon ( Salmo salar ), Fish and Shellfish Immunology, 122 pp. 437-445. ISSN 1050-4648 (2022) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/35189323 http://ecite.utas.edu.au/152037 |
op_doi |
https://doi.org/10.1016/j.fsi.2022.02.035 |
container_title |
Fish & Shellfish Immunology |
container_volume |
122 |
container_start_page |
437 |
op_container_end_page |
445 |
_version_ |
1766358691289759744 |