High-throughput real-time PCR and melt curve analysis for sexing Southern Ocean seabirds using fecal samples
Sex identification of birds is of great interest in ecological studies, however this can be very difficult in many species because their external features are almost monomorphic between the sexes. Molecular methodology has simplified this process but limitations still occur with widely accepted meth...
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2014
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Online Access: | https://doi.org/10.1016/j.theriogenology.2013.12.021 http://www.ncbi.nlm.nih.gov/pubmed/24507073 http://ecite.utas.edu.au/148758 |
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ftunivtasecite:oai:ecite.utas.edu.au:148758 2023-05-15T18:24:50+02:00 High-throughput real-time PCR and melt curve analysis for sexing Southern Ocean seabirds using fecal samples Faux, CE McInnes, JC Jarman, SN 2014 application/pdf https://doi.org/10.1016/j.theriogenology.2013.12.021 http://www.ncbi.nlm.nih.gov/pubmed/24507073 http://ecite.utas.edu.au/148758 en eng Elsevier Science Inc http://ecite.utas.edu.au/148758/1/148758 - High-throughput real-time PCR and melt curve analysis.pdf http://dx.doi.org/10.1016/j.theriogenology.2013.12.021 Faux, CE and McInnes, JC and Jarman, SN, High-throughput real-time PCR and melt curve analysis for sexing Southern Ocean seabirds using fecal samples, Theriogenology, 81, (6) pp. 870-874. ISSN 0093-691X (2014) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/24507073 http://ecite.utas.edu.au/148758 Biological Sciences Ecology Marine and estuarine ecology (incl. marine ichthyology) Refereed Article PeerReviewed 2014 ftunivtasecite https://doi.org/10.1016/j.theriogenology.2013.12.021 2022-03-14T23:16:42Z Sex identification of birds is of great interest in ecological studies, however this can be very difficult in many species because their external features are almost monomorphic between the sexes. Molecular methodology has simplified this process but limitations still occur with widely accepted methods using polymerase chain reaction and gel electrophoresis , especially when applied to degraded DNA. Real-time polymerase chain reaction assays are emerging as a more efficient, sensitive, and higher throughput means of identification, but there are very few techniques validated using fecal samples and small target sizes. We present a real-time melt curve analysis assay targeting a small region of the CHD-1 gene allowing for high-throughput, sensitive, specific, and easy-to-interpret sexing results for a variety of Southern Ocean seabirds using fecal and tissue samples. Article in Journal/Newspaper Southern Ocean eCite UTAS (University of Tasmania) Southern Ocean Theriogenology 81 6 870 874 |
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Open Polar |
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eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Biological Sciences Ecology Marine and estuarine ecology (incl. marine ichthyology) |
spellingShingle |
Biological Sciences Ecology Marine and estuarine ecology (incl. marine ichthyology) Faux, CE McInnes, JC Jarman, SN High-throughput real-time PCR and melt curve analysis for sexing Southern Ocean seabirds using fecal samples |
topic_facet |
Biological Sciences Ecology Marine and estuarine ecology (incl. marine ichthyology) |
description |
Sex identification of birds is of great interest in ecological studies, however this can be very difficult in many species because their external features are almost monomorphic between the sexes. Molecular methodology has simplified this process but limitations still occur with widely accepted methods using polymerase chain reaction and gel electrophoresis , especially when applied to degraded DNA. Real-time polymerase chain reaction assays are emerging as a more efficient, sensitive, and higher throughput means of identification, but there are very few techniques validated using fecal samples and small target sizes. We present a real-time melt curve analysis assay targeting a small region of the CHD-1 gene allowing for high-throughput, sensitive, specific, and easy-to-interpret sexing results for a variety of Southern Ocean seabirds using fecal and tissue samples. |
format |
Article in Journal/Newspaper |
author |
Faux, CE McInnes, JC Jarman, SN |
author_facet |
Faux, CE McInnes, JC Jarman, SN |
author_sort |
Faux, CE |
title |
High-throughput real-time PCR and melt curve analysis for sexing Southern Ocean seabirds using fecal samples |
title_short |
High-throughput real-time PCR and melt curve analysis for sexing Southern Ocean seabirds using fecal samples |
title_full |
High-throughput real-time PCR and melt curve analysis for sexing Southern Ocean seabirds using fecal samples |
title_fullStr |
High-throughput real-time PCR and melt curve analysis for sexing Southern Ocean seabirds using fecal samples |
title_full_unstemmed |
High-throughput real-time PCR and melt curve analysis for sexing Southern Ocean seabirds using fecal samples |
title_sort |
high-throughput real-time pcr and melt curve analysis for sexing southern ocean seabirds using fecal samples |
publisher |
Elsevier Science Inc |
publishDate |
2014 |
url |
https://doi.org/10.1016/j.theriogenology.2013.12.021 http://www.ncbi.nlm.nih.gov/pubmed/24507073 http://ecite.utas.edu.au/148758 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_relation |
http://ecite.utas.edu.au/148758/1/148758 - High-throughput real-time PCR and melt curve analysis.pdf http://dx.doi.org/10.1016/j.theriogenology.2013.12.021 Faux, CE and McInnes, JC and Jarman, SN, High-throughput real-time PCR and melt curve analysis for sexing Southern Ocean seabirds using fecal samples, Theriogenology, 81, (6) pp. 870-874. ISSN 0093-691X (2014) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/24507073 http://ecite.utas.edu.au/148758 |
op_doi |
https://doi.org/10.1016/j.theriogenology.2013.12.021 |
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Theriogenology |
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81 |
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6 |
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870 |
op_container_end_page |
874 |
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1766205781439414272 |