Profiling branchial bacteria of Atlantic salmon ( Salmo salar L.) following exposure to antimicrobial agents
Microbial gill diseases caused by either opportunistic or specific pathogens are an emerging area of concern for aquaculture producers in part due to their sometimes complex and/or cryptic nature. Many antimicrobial treatments used in aquacultural settings are broad spectrum in nature. The effect of...
Published in: | Frontiers in Animal Science |
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Online Access: | https://doi.org/10.3389/fanim.2021.756101 http://ecite.utas.edu.au/147461 |
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ftunivtasecite:oai:ecite.utas.edu.au:147461 2023-05-15T15:30:52+02:00 Profiling branchial bacteria of Atlantic salmon ( Salmo salar L.) following exposure to antimicrobial agents Slinger, J Wynne, JW Adams, MB 2021 application/pdf https://doi.org/10.3389/fanim.2021.756101 http://ecite.utas.edu.au/147461 en eng Frontiers Media SA http://ecite.utas.edu.au/147461/1/147461 - Profiling branchial bacteria of Atlantic salmon.pdf http://dx.doi.org/10.3389/fanim.2021.756101 Slinger, J and Wynne, JW and Adams, MB, Profiling branchial bacteria of Atlantic salmon ( Salmo salar L.) following exposure to antimicrobial agents, Frontiers in Animal Science, 2 Article 756101. ISSN 2673-6225 (2021) [Refereed Article] http://ecite.utas.edu.au/147461 Agricultural Veterinary and Food Sciences Fisheries sciences Aquaculture Refereed Article PeerReviewed 2021 ftunivtasecite https://doi.org/10.3389/fanim.2021.756101 2021-12-13T23:17:58Z Microbial gill diseases caused by either opportunistic or specific pathogens are an emerging area of concern for aquaculture producers in part due to their sometimes complex and/or cryptic nature. Many antimicrobial treatments used in aquacultural settings are broad spectrum in nature. The effect of such therapeutics upon reduction and recolonization of commensal or pathogenic microbiota post-treatment has received little attention to date. Commensal bacteria are an integral component of the barrier function of mucosal surfaces in animals. This study evaluated the effect of several commercially relevant antimicrobial treatments upon the diversity and composition of branchial bacteria of Atlantic salmon. Here we exposed Atlantic salmon smolt to a number of commercially relevant antimicrobial treatments including chemotherapeutants (chloramine-t and hydrogen peroxide) and antibiotics (oxytetracycline and florfenicol) in vivo . Subsequently we examined the change in bacterial load, 16S rRNA gene expression, and taxonomic diversity post-treatment upon the gills. Results revealed a decrease in cultivable bacterial colonies after antimicrobial treatment, and a downstream decrease in bacterial richness and abundance post-treatment, with colonization of several prominent pathogenic taxa including Vibrio and Tenacibaculum . Temporal tracing over a 14-day period demonstrated that the bacteriome of gill mucus is sensitive to change, and altered by antimicrobial treatment and handling. This study identified candidate antimicrobial treatments which could be implemented in future studies to illustrate the effect of dysbiosis on microbial gill diseases. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Frontiers in Animal Science 2 |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
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English |
topic |
Agricultural Veterinary and Food Sciences Fisheries sciences Aquaculture |
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Agricultural Veterinary and Food Sciences Fisheries sciences Aquaculture Slinger, J Wynne, JW Adams, MB Profiling branchial bacteria of Atlantic salmon ( Salmo salar L.) following exposure to antimicrobial agents |
topic_facet |
Agricultural Veterinary and Food Sciences Fisheries sciences Aquaculture |
description |
Microbial gill diseases caused by either opportunistic or specific pathogens are an emerging area of concern for aquaculture producers in part due to their sometimes complex and/or cryptic nature. Many antimicrobial treatments used in aquacultural settings are broad spectrum in nature. The effect of such therapeutics upon reduction and recolonization of commensal or pathogenic microbiota post-treatment has received little attention to date. Commensal bacteria are an integral component of the barrier function of mucosal surfaces in animals. This study evaluated the effect of several commercially relevant antimicrobial treatments upon the diversity and composition of branchial bacteria of Atlantic salmon. Here we exposed Atlantic salmon smolt to a number of commercially relevant antimicrobial treatments including chemotherapeutants (chloramine-t and hydrogen peroxide) and antibiotics (oxytetracycline and florfenicol) in vivo . Subsequently we examined the change in bacterial load, 16S rRNA gene expression, and taxonomic diversity post-treatment upon the gills. Results revealed a decrease in cultivable bacterial colonies after antimicrobial treatment, and a downstream decrease in bacterial richness and abundance post-treatment, with colonization of several prominent pathogenic taxa including Vibrio and Tenacibaculum . Temporal tracing over a 14-day period demonstrated that the bacteriome of gill mucus is sensitive to change, and altered by antimicrobial treatment and handling. This study identified candidate antimicrobial treatments which could be implemented in future studies to illustrate the effect of dysbiosis on microbial gill diseases. |
format |
Article in Journal/Newspaper |
author |
Slinger, J Wynne, JW Adams, MB |
author_facet |
Slinger, J Wynne, JW Adams, MB |
author_sort |
Slinger, J |
title |
Profiling branchial bacteria of Atlantic salmon ( Salmo salar L.) following exposure to antimicrobial agents |
title_short |
Profiling branchial bacteria of Atlantic salmon ( Salmo salar L.) following exposure to antimicrobial agents |
title_full |
Profiling branchial bacteria of Atlantic salmon ( Salmo salar L.) following exposure to antimicrobial agents |
title_fullStr |
Profiling branchial bacteria of Atlantic salmon ( Salmo salar L.) following exposure to antimicrobial agents |
title_full_unstemmed |
Profiling branchial bacteria of Atlantic salmon ( Salmo salar L.) following exposure to antimicrobial agents |
title_sort |
profiling branchial bacteria of atlantic salmon ( salmo salar l.) following exposure to antimicrobial agents |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
https://doi.org/10.3389/fanim.2021.756101 http://ecite.utas.edu.au/147461 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://ecite.utas.edu.au/147461/1/147461 - Profiling branchial bacteria of Atlantic salmon.pdf http://dx.doi.org/10.3389/fanim.2021.756101 Slinger, J and Wynne, JW and Adams, MB, Profiling branchial bacteria of Atlantic salmon ( Salmo salar L.) following exposure to antimicrobial agents, Frontiers in Animal Science, 2 Article 756101. ISSN 2673-6225 (2021) [Refereed Article] http://ecite.utas.edu.au/147461 |
op_doi |
https://doi.org/10.3389/fanim.2021.756101 |
container_title |
Frontiers in Animal Science |
container_volume |
2 |
_version_ |
1766361328820158464 |