Serological screening for Brucella spp. and Leptospira spp. antibodies in southern elephant seals Mirounga leonina from Elephant Island, Antarctica, in 2003 and 2004
Brucella spp. and Leptospira spp. antibodies were surveyed in 35 southern elephant seals (SESs) Mirounga leonina at Elephant Island (South Shetland Islands), western Antarctic peninsula, in the Austral summer of 2003 and 2004. The rose Bengal test and a commercial competitive ELISA (c-ELISA) were us...
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Online Access: | https://doi.org/10.3354/DAO03548 http://www.ncbi.nlm.nih.gov/pubmed/33331283 http://ecite.utas.edu.au/143581 |
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ftunivtasecite:oai:ecite.utas.edu.au:143581 2023-05-15T13:59:46+02:00 Serological screening for Brucella spp. and Leptospira spp. antibodies in southern elephant seals Mirounga leonina from Elephant Island, Antarctica, in 2003 and 2004 Sanchez-Sarmiento, AM Ruoppolo, V Muelbert, MMC Neto, JSF Catao-Dias, JL 2020 https://doi.org/10.3354/DAO03548 http://www.ncbi.nlm.nih.gov/pubmed/33331283 http://ecite.utas.edu.au/143581 en eng Inter-Research http://dx.doi.org/10.3354/DAO03548 Sanchez-Sarmiento, AM and Ruoppolo, V and Muelbert, MMC and Neto, JSF and Catao-Dias, JL, Serological screening for Brucella spp. and Leptospira spp. antibodies in southern elephant seals Mirounga leonina from Elephant Island, Antarctica, in 2003 and 2004, Diseases of Aquatic Organisms, 142 pp. 161-170. ISSN 0177-5103 (2020) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/33331283 http://ecite.utas.edu.au/143581 Biological Sciences Evolutionary biology Host-parasite interactions Refereed Article PeerReviewed 2020 ftunivtasecite https://doi.org/10.3354/DAO03548 2021-06-28T22:16:33Z Brucella spp. and Leptospira spp. antibodies were surveyed in 35 southern elephant seals (SESs) Mirounga leonina at Elephant Island (South Shetland Islands), western Antarctic peninsula, in the Austral summer of 2003 and 2004. The rose Bengal test and a commercial competitive ELISA (c-ELISA) were used to detect Brucella spp. exposure, and the microscopic agglutination test (MAT) with 22 live serovars was used to determine anti- Leptospira spp. antibodies. We found evidence of Brucella spp. exposure in 3 of 35 (8.6%) SESs tested via the c-ELISA displaying high percentage inhibition (PI), similar to other studies in pinnipeds in which Brucella spp. antibodies have been determined. Two of the 3 positives were pups (PI = 70.4 and 86.6%), while the third was an adult female (PI = 48.8%). The 3 c-ELISA positive SESs were additionally tested via the serum agglutination test but were found to be negative. All individuals were negative for antibodies against 22 Leptospira spp. serovars by MAT. These results contribute to the knowledge and monitoring of zoonotic pathogens with epizootic potential in Southern Ocean pinnipeds. Given the potential impact that pathogens may have on the abundance of wild (sometimes threatened and endangered) populations, constant monitoring and surveillance are required to prevent pathogen spread, particularly under forecast climate change scenarios. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Elephant Island Elephant Seals Mirounga leonina South Shetland Islands Southern Elephant Seals Southern Ocean eCite UTAS (University of Tasmania) Antarctic Southern Ocean Antarctic Peninsula Austral South Shetland Islands Elephant Island ENVELOPE(-55.184,-55.184,-61.085,-61.085) Diseases of Aquatic Organisms 142 161 170 |
institution |
Open Polar |
collection |
eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Biological Sciences Evolutionary biology Host-parasite interactions |
spellingShingle |
Biological Sciences Evolutionary biology Host-parasite interactions Sanchez-Sarmiento, AM Ruoppolo, V Muelbert, MMC Neto, JSF Catao-Dias, JL Serological screening for Brucella spp. and Leptospira spp. antibodies in southern elephant seals Mirounga leonina from Elephant Island, Antarctica, in 2003 and 2004 |
topic_facet |
Biological Sciences Evolutionary biology Host-parasite interactions |
description |
Brucella spp. and Leptospira spp. antibodies were surveyed in 35 southern elephant seals (SESs) Mirounga leonina at Elephant Island (South Shetland Islands), western Antarctic peninsula, in the Austral summer of 2003 and 2004. The rose Bengal test and a commercial competitive ELISA (c-ELISA) were used to detect Brucella spp. exposure, and the microscopic agglutination test (MAT) with 22 live serovars was used to determine anti- Leptospira spp. antibodies. We found evidence of Brucella spp. exposure in 3 of 35 (8.6%) SESs tested via the c-ELISA displaying high percentage inhibition (PI), similar to other studies in pinnipeds in which Brucella spp. antibodies have been determined. Two of the 3 positives were pups (PI = 70.4 and 86.6%), while the third was an adult female (PI = 48.8%). The 3 c-ELISA positive SESs were additionally tested via the serum agglutination test but were found to be negative. All individuals were negative for antibodies against 22 Leptospira spp. serovars by MAT. These results contribute to the knowledge and monitoring of zoonotic pathogens with epizootic potential in Southern Ocean pinnipeds. Given the potential impact that pathogens may have on the abundance of wild (sometimes threatened and endangered) populations, constant monitoring and surveillance are required to prevent pathogen spread, particularly under forecast climate change scenarios. |
format |
Article in Journal/Newspaper |
author |
Sanchez-Sarmiento, AM Ruoppolo, V Muelbert, MMC Neto, JSF Catao-Dias, JL |
author_facet |
Sanchez-Sarmiento, AM Ruoppolo, V Muelbert, MMC Neto, JSF Catao-Dias, JL |
author_sort |
Sanchez-Sarmiento, AM |
title |
Serological screening for Brucella spp. and Leptospira spp. antibodies in southern elephant seals Mirounga leonina from Elephant Island, Antarctica, in 2003 and 2004 |
title_short |
Serological screening for Brucella spp. and Leptospira spp. antibodies in southern elephant seals Mirounga leonina from Elephant Island, Antarctica, in 2003 and 2004 |
title_full |
Serological screening for Brucella spp. and Leptospira spp. antibodies in southern elephant seals Mirounga leonina from Elephant Island, Antarctica, in 2003 and 2004 |
title_fullStr |
Serological screening for Brucella spp. and Leptospira spp. antibodies in southern elephant seals Mirounga leonina from Elephant Island, Antarctica, in 2003 and 2004 |
title_full_unstemmed |
Serological screening for Brucella spp. and Leptospira spp. antibodies in southern elephant seals Mirounga leonina from Elephant Island, Antarctica, in 2003 and 2004 |
title_sort |
serological screening for brucella spp. and leptospira spp. antibodies in southern elephant seals mirounga leonina from elephant island, antarctica, in 2003 and 2004 |
publisher |
Inter-Research |
publishDate |
2020 |
url |
https://doi.org/10.3354/DAO03548 http://www.ncbi.nlm.nih.gov/pubmed/33331283 http://ecite.utas.edu.au/143581 |
long_lat |
ENVELOPE(-55.184,-55.184,-61.085,-61.085) |
geographic |
Antarctic Southern Ocean Antarctic Peninsula Austral South Shetland Islands Elephant Island |
geographic_facet |
Antarctic Southern Ocean Antarctic Peninsula Austral South Shetland Islands Elephant Island |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Elephant Island Elephant Seals Mirounga leonina South Shetland Islands Southern Elephant Seals Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Elephant Island Elephant Seals Mirounga leonina South Shetland Islands Southern Elephant Seals Southern Ocean |
op_relation |
http://dx.doi.org/10.3354/DAO03548 Sanchez-Sarmiento, AM and Ruoppolo, V and Muelbert, MMC and Neto, JSF and Catao-Dias, JL, Serological screening for Brucella spp. and Leptospira spp. antibodies in southern elephant seals Mirounga leonina from Elephant Island, Antarctica, in 2003 and 2004, Diseases of Aquatic Organisms, 142 pp. 161-170. ISSN 0177-5103 (2020) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/33331283 http://ecite.utas.edu.au/143581 |
op_doi |
https://doi.org/10.3354/DAO03548 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
142 |
container_start_page |
161 |
op_container_end_page |
170 |
_version_ |
1766268560314728448 |