Comparison of bacterial diversity and distribution on the gills of Atlantic salmon ( Salmo salar L.): an evaluation of sampling techniques
<section class="article-section__content" id="jam14969-sec-0001"> Aims Assess bacterial diversity and richness in mucus samples from the gills of Atlantic salmon in comparison to preserved or fixed gill filament tissues. Ascertain whether bacterial diversity and richness ar...
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2021
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Online Access: | https://doi.org/10.1111/jam.14969 http://www.ncbi.nlm.nih.gov/pubmed/33326661 http://ecite.utas.edu.au/142396 |
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ftunivtasecite:oai:ecite.utas.edu.au:142396 2023-05-15T15:31:47+02:00 Comparison of bacterial diversity and distribution on the gills of Atlantic salmon ( Salmo salar L.): an evaluation of sampling techniques Slinger, J Adams, MB Wynne, JW 2021 https://doi.org/10.1111/jam.14969 http://www.ncbi.nlm.nih.gov/pubmed/33326661 http://ecite.utas.edu.au/142396 en eng Blackwell Publishing Ltd http://dx.doi.org/10.1111/jam.14969 Slinger, J and Adams, MB and Wynne, JW, Comparison of bacterial diversity and distribution on the gills of Atlantic salmon ( Salmo salar L.): an evaluation of sampling techniques, Journal of Applied Microbiology, 131, (1) pp. 80-92. ISSN 1364-5072 (2021) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/33326661 http://ecite.utas.edu.au/142396 Biological Sciences Microbiology Bacteriology Refereed Article PeerReviewed 2021 ftunivtasecite https://doi.org/10.1111/jam.14969 2021-11-01T23:17:54Z <section class="article-section__content" id="jam14969-sec-0001"> Aims Assess bacterial diversity and richness in mucus samples from the gills of Atlantic salmon in comparison to preserved or fixed gill filament tissues. Ascertain whether bacterial diversity and richness are homogeneous upon different arches of the gill basket. </section> <section class="article-section__content" id="jam14969-sec-0002"> Methods and Results Bacterial communities contained within gill mucus were profiled using 16S rRNA gene sequencing. No significant difference in taxa richness, alpha ( P >005) or beta diversity indices ( P >005) were found between the bacterial communities of RNAlater preserved gill tissues and swab‐bound mucus. A trend of lower richness and diversity indices were observed in bacterial communities from posterior hemibranchs. </section> <section class="article-section__content" id="jam14969-sec-0003"> Conclusions Non‐lethal swab sampling of gill mucus provides a robust representation of bacterial communities externally upon the gills. Bacterial communities from the fourth arch appeared to be the least representative overall. </section> <section class="article-section__content" id="jam14969-sec-0004"> Significance and Impact of the Study The external mucosal barriers of teleost fish (e.g. gill surface) play a vital role as a primary defence line against infection. While research effort on the role of microbial communities on health and immunity of aquaculture species continues, the collection and sampling processes to obtain these data require evaluation so methodologies are consistently applied across future studies that aim to evaluate the composition of branchial microbiomes. </section> Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Journal of Applied Microbiology 131 1 80 92 |
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Open Polar |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Biological Sciences Microbiology Bacteriology |
spellingShingle |
Biological Sciences Microbiology Bacteriology Slinger, J Adams, MB Wynne, JW Comparison of bacterial diversity and distribution on the gills of Atlantic salmon ( Salmo salar L.): an evaluation of sampling techniques |
topic_facet |
Biological Sciences Microbiology Bacteriology |
description |
<section class="article-section__content" id="jam14969-sec-0001"> Aims Assess bacterial diversity and richness in mucus samples from the gills of Atlantic salmon in comparison to preserved or fixed gill filament tissues. Ascertain whether bacterial diversity and richness are homogeneous upon different arches of the gill basket. </section> <section class="article-section__content" id="jam14969-sec-0002"> Methods and Results Bacterial communities contained within gill mucus were profiled using 16S rRNA gene sequencing. No significant difference in taxa richness, alpha ( P >005) or beta diversity indices ( P >005) were found between the bacterial communities of RNAlater preserved gill tissues and swab‐bound mucus. A trend of lower richness and diversity indices were observed in bacterial communities from posterior hemibranchs. </section> <section class="article-section__content" id="jam14969-sec-0003"> Conclusions Non‐lethal swab sampling of gill mucus provides a robust representation of bacterial communities externally upon the gills. Bacterial communities from the fourth arch appeared to be the least representative overall. </section> <section class="article-section__content" id="jam14969-sec-0004"> Significance and Impact of the Study The external mucosal barriers of teleost fish (e.g. gill surface) play a vital role as a primary defence line against infection. While research effort on the role of microbial communities on health and immunity of aquaculture species continues, the collection and sampling processes to obtain these data require evaluation so methodologies are consistently applied across future studies that aim to evaluate the composition of branchial microbiomes. </section> |
format |
Article in Journal/Newspaper |
author |
Slinger, J Adams, MB Wynne, JW |
author_facet |
Slinger, J Adams, MB Wynne, JW |
author_sort |
Slinger, J |
title |
Comparison of bacterial diversity and distribution on the gills of Atlantic salmon ( Salmo salar L.): an evaluation of sampling techniques |
title_short |
Comparison of bacterial diversity and distribution on the gills of Atlantic salmon ( Salmo salar L.): an evaluation of sampling techniques |
title_full |
Comparison of bacterial diversity and distribution on the gills of Atlantic salmon ( Salmo salar L.): an evaluation of sampling techniques |
title_fullStr |
Comparison of bacterial diversity and distribution on the gills of Atlantic salmon ( Salmo salar L.): an evaluation of sampling techniques |
title_full_unstemmed |
Comparison of bacterial diversity and distribution on the gills of Atlantic salmon ( Salmo salar L.): an evaluation of sampling techniques |
title_sort |
comparison of bacterial diversity and distribution on the gills of atlantic salmon ( salmo salar l.): an evaluation of sampling techniques |
publisher |
Blackwell Publishing Ltd |
publishDate |
2021 |
url |
https://doi.org/10.1111/jam.14969 http://www.ncbi.nlm.nih.gov/pubmed/33326661 http://ecite.utas.edu.au/142396 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://dx.doi.org/10.1111/jam.14969 Slinger, J and Adams, MB and Wynne, JW, Comparison of bacterial diversity and distribution on the gills of Atlantic salmon ( Salmo salar L.): an evaluation of sampling techniques, Journal of Applied Microbiology, 131, (1) pp. 80-92. ISSN 1364-5072 (2021) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/33326661 http://ecite.utas.edu.au/142396 |
op_doi |
https://doi.org/10.1111/jam.14969 |
container_title |
Journal of Applied Microbiology |
container_volume |
131 |
container_issue |
1 |
container_start_page |
80 |
op_container_end_page |
92 |
_version_ |
1766362295568433152 |