Rapid manipulation in irradiance induces oxidative free-radical release in a fast-ice algal community (McMurdo Sound, Antarctica)
Sea ice supports a unique assemblage of microorganisms that underpin Antarctic coastal food-webs, but reduced ice thickness coupled with increased snow cover will modify energy flow and could lead to photodamage in ice-associated microalgae. In this study, microsensors were used to examine the influ...
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Online Access: | https://doi.org/10.3389/fpls.2020.588005 http://ecite.utas.edu.au/141884 |
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ftunivtasecite:oai:ecite.utas.edu.au:141884 2023-05-15T13:42:40+02:00 Rapid manipulation in irradiance induces oxidative free-radical release in a fast-ice algal community (McMurdo Sound, Antarctica) Kennedy, F Martin, A Castrisios, K Cimoli, E McMinn, A Ryan, KG 2020 application/pdf https://doi.org/10.3389/fpls.2020.588005 http://ecite.utas.edu.au/141884 en eng Frontiers Research Foundation http://ecite.utas.edu.au/141884/1/141884 - Rapid manipulation in irradiance induces oxidative free-radical release.pdf http://dx.doi.org/10.3389/fpls.2020.588005 Kennedy, F and Martin, A and Castrisios, K and Cimoli, E and McMinn, A and Ryan, KG, Rapid manipulation in irradiance induces oxidative free-radical release in a fast-ice algal community (McMurdo Sound, Antarctica), Frontiers in Plant Science, 11 Article 588005. ISSN 1664-462X (2020) [Refereed Article] http://ecite.utas.edu.au/141884 Biological Sciences Biochemistry and cell biology Cell metabolism Refereed Article PeerReviewed 2020 ftunivtasecite https://doi.org/10.3389/fpls.2020.588005 2022-08-29T22:18:09Z Sea ice supports a unique assemblage of microorganisms that underpin Antarctic coastal food-webs, but reduced ice thickness coupled with increased snow cover will modify energy flow and could lead to photodamage in ice-associated microalgae. In this study, microsensors were used to examine the influence of rapid shifts in irradiance on extracellular oxidative free radicals produced by sea-ice algae. Bottom-ice algal communities were exposed to one of three levels of incident light for 10 days: low (0.5 μmol photons m −2 s −1 , 30 cm snow cover), mid-range (5 μmol photons m −2 s −1 , 10 cm snow), or high light (13 μmol photons m −2 s −1 , no snow). After 10 days, the snow cover was reversed (either removed or added), resulting in a rapid change in irradiance at the ice-water interface. In treatments acclimated to low light, the subsequent exposure to high irradiance resulted in a ~400 increase in the production of hydrogen peroxide (H 2 O 2 ) and a 10 increase in nitric oxide (NO) concentration after 24 h. The observed increase in oxidative free radicals also resulted in significant changes in photosynthetic electron flow, RNA-oxidative damage, and community structural dynamics. In contrast, there was no significant response in sea-ice algae acclimated to high light and then exposed to a significantly lower irradiance at either 24 or 72 h. Our results demonstrate that microsensors can be used to track real-time in-situ stress in sea-ice microbial communities. Extrapolating to ecologically relevant spatiotemporal scales remains a significant challenge, but this approach offers a fundamentally enhanced level of resolution for quantifying the microbial response to global change. Article in Journal/Newspaper Antarc* Antarctic Antarctica ice algae McMurdo Sound Sea ice eCite UTAS (University of Tasmania) Antarctic McMurdo Sound Frontiers in Plant Science 11 |
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Open Polar |
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eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Biological Sciences Biochemistry and cell biology Cell metabolism |
spellingShingle |
Biological Sciences Biochemistry and cell biology Cell metabolism Kennedy, F Martin, A Castrisios, K Cimoli, E McMinn, A Ryan, KG Rapid manipulation in irradiance induces oxidative free-radical release in a fast-ice algal community (McMurdo Sound, Antarctica) |
topic_facet |
Biological Sciences Biochemistry and cell biology Cell metabolism |
description |
Sea ice supports a unique assemblage of microorganisms that underpin Antarctic coastal food-webs, but reduced ice thickness coupled with increased snow cover will modify energy flow and could lead to photodamage in ice-associated microalgae. In this study, microsensors were used to examine the influence of rapid shifts in irradiance on extracellular oxidative free radicals produced by sea-ice algae. Bottom-ice algal communities were exposed to one of three levels of incident light for 10 days: low (0.5 μmol photons m −2 s −1 , 30 cm snow cover), mid-range (5 μmol photons m −2 s −1 , 10 cm snow), or high light (13 μmol photons m −2 s −1 , no snow). After 10 days, the snow cover was reversed (either removed or added), resulting in a rapid change in irradiance at the ice-water interface. In treatments acclimated to low light, the subsequent exposure to high irradiance resulted in a ~400 increase in the production of hydrogen peroxide (H 2 O 2 ) and a 10 increase in nitric oxide (NO) concentration after 24 h. The observed increase in oxidative free radicals also resulted in significant changes in photosynthetic electron flow, RNA-oxidative damage, and community structural dynamics. In contrast, there was no significant response in sea-ice algae acclimated to high light and then exposed to a significantly lower irradiance at either 24 or 72 h. Our results demonstrate that microsensors can be used to track real-time in-situ stress in sea-ice microbial communities. Extrapolating to ecologically relevant spatiotemporal scales remains a significant challenge, but this approach offers a fundamentally enhanced level of resolution for quantifying the microbial response to global change. |
format |
Article in Journal/Newspaper |
author |
Kennedy, F Martin, A Castrisios, K Cimoli, E McMinn, A Ryan, KG |
author_facet |
Kennedy, F Martin, A Castrisios, K Cimoli, E McMinn, A Ryan, KG |
author_sort |
Kennedy, F |
title |
Rapid manipulation in irradiance induces oxidative free-radical release in a fast-ice algal community (McMurdo Sound, Antarctica) |
title_short |
Rapid manipulation in irradiance induces oxidative free-radical release in a fast-ice algal community (McMurdo Sound, Antarctica) |
title_full |
Rapid manipulation in irradiance induces oxidative free-radical release in a fast-ice algal community (McMurdo Sound, Antarctica) |
title_fullStr |
Rapid manipulation in irradiance induces oxidative free-radical release in a fast-ice algal community (McMurdo Sound, Antarctica) |
title_full_unstemmed |
Rapid manipulation in irradiance induces oxidative free-radical release in a fast-ice algal community (McMurdo Sound, Antarctica) |
title_sort |
rapid manipulation in irradiance induces oxidative free-radical release in a fast-ice algal community (mcmurdo sound, antarctica) |
publisher |
Frontiers Research Foundation |
publishDate |
2020 |
url |
https://doi.org/10.3389/fpls.2020.588005 http://ecite.utas.edu.au/141884 |
geographic |
Antarctic McMurdo Sound |
geographic_facet |
Antarctic McMurdo Sound |
genre |
Antarc* Antarctic Antarctica ice algae McMurdo Sound Sea ice |
genre_facet |
Antarc* Antarctic Antarctica ice algae McMurdo Sound Sea ice |
op_relation |
http://ecite.utas.edu.au/141884/1/141884 - Rapid manipulation in irradiance induces oxidative free-radical release.pdf http://dx.doi.org/10.3389/fpls.2020.588005 Kennedy, F and Martin, A and Castrisios, K and Cimoli, E and McMinn, A and Ryan, KG, Rapid manipulation in irradiance induces oxidative free-radical release in a fast-ice algal community (McMurdo Sound, Antarctica), Frontiers in Plant Science, 11 Article 588005. ISSN 1664-462X (2020) [Refereed Article] http://ecite.utas.edu.au/141884 |
op_doi |
https://doi.org/10.3389/fpls.2020.588005 |
container_title |
Frontiers in Plant Science |
container_volume |
11 |
_version_ |
1766171184605429760 |