Unexpected host dependency of Antarctic Nanohaloarchaeota
In hypersaline environments, Nanohaloarchaeota (Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoarchaeota, Nanohaloarchaeota [DPANN] superphylum) are thought to be free-living microorganisms. We report cultivation of 2 strains of Antarctic Nanohaloarchaeota and show that they require the haloarc...
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Online Access: | https://doi.org/10.1073/pnas.1905179116 http://www.ncbi.nlm.nih.gov/pubmed/31253704 http://ecite.utas.edu.au/137594 |
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ftunivtasecite:oai:ecite.utas.edu.au:137594 2023-05-15T13:55:18+02:00 Unexpected host dependency of Antarctic Nanohaloarchaeota Hamm, JN Erdmann, S Eloe-Fadrosh, EA Angeloni, A Zhong, L Brownlee, C Williams, TJ Barton, K Carswell, S Smith, MA Brazendale, S Hancock, AM Allen, MA Raftery, MJ Cavicchioli, R 2019 application/pdf https://doi.org/10.1073/pnas.1905179116 http://www.ncbi.nlm.nih.gov/pubmed/31253704 http://ecite.utas.edu.au/137594 en eng Natl Acad Sciences http://ecite.utas.edu.au/137594/1/137594 - Unexpected host dependency of Antarctic Nanohaloarchaeota.pdf http://dx.doi.org/10.1073/pnas.1905179116 Hamm, JN and Erdmann, S and Eloe-Fadrosh, EA and Angeloni, A and Zhong, L and Brownlee, C and Williams, TJ and Barton, K and Carswell, S and Smith, MA and Brazendale, S and Hancock, AM and Allen, MA and Raftery, MJ and Cavicchioli, R, Unexpected host dependency of Antarctic Nanohaloarchaeota, Proceedings of the National Academy of Sciences of the United States of America, 116, (29) pp. 14661-14670. ISSN 0027-8424 (2019) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/31253704 http://ecite.utas.edu.au/137594 Biological Sciences Ecology Marine and Estuarine Ecology (incl. Marine Ichthyology) Refereed Article PeerReviewed 2019 ftunivtasecite https://doi.org/10.1073/pnas.1905179116 2020-03-30T22:16:24Z In hypersaline environments, Nanohaloarchaeota (Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoarchaeota, Nanohaloarchaeota [DPANN] superphylum) are thought to be free-living microorganisms. We report cultivation of 2 strains of Antarctic Nanohaloarchaeota and show that they require the haloarchaeon Halorubrum lacusprofundi for growth. By performing growth using enrichments and fluorescence-activated cell sorting, we demonstrated successful cultivation of Candidatus Nanohaloarchaeum antarcticus, purification of Ca. Nha. antarcticus away from other species, and growth and verification of Ca. Nha. antarcticus with Hrr. lacusprofundi these findings are analogous to those required for fulfilling Kochs postulates. We use fluorescent in situ hybridization and transmission electron microscopy to assess cell structures and interactions; metagenomics to characterize enrichment taxa, generate metagenome assembled genomes, and interrogate Antarctic communities; and proteomics to assess metabolic pathways and speculate about the roles of certain proteins. Metagenome analysis indicates the presence of a single species, which is endemic to Antarctic hypersaline systems that support the growth of haloarchaea. The presence of unusually large proteins predicted to function in attachment and invasion of hosts plus the absence of key biosynthetic pathways (e.g., lipids) in metagenome assembled genomes of globally distributed Nanohaloarchaeota indicate that all members of the lineage have evolved as symbionts. Our work expands the range of archaeal symbiotic lifestyles and provides a genetically tractable model system for advancing understanding of the factors controlling microbial symbiotic relationships. Article in Journal/Newspaper Antarc* Antarctic antarcticus eCite UTAS (University of Tasmania) Antarctic Proceedings of the National Academy of Sciences 116 29 14661 14670 |
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Open Polar |
collection |
eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Biological Sciences Ecology Marine and Estuarine Ecology (incl. Marine Ichthyology) |
spellingShingle |
Biological Sciences Ecology Marine and Estuarine Ecology (incl. Marine Ichthyology) Hamm, JN Erdmann, S Eloe-Fadrosh, EA Angeloni, A Zhong, L Brownlee, C Williams, TJ Barton, K Carswell, S Smith, MA Brazendale, S Hancock, AM Allen, MA Raftery, MJ Cavicchioli, R Unexpected host dependency of Antarctic Nanohaloarchaeota |
topic_facet |
Biological Sciences Ecology Marine and Estuarine Ecology (incl. Marine Ichthyology) |
description |
In hypersaline environments, Nanohaloarchaeota (Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoarchaeota, Nanohaloarchaeota [DPANN] superphylum) are thought to be free-living microorganisms. We report cultivation of 2 strains of Antarctic Nanohaloarchaeota and show that they require the haloarchaeon Halorubrum lacusprofundi for growth. By performing growth using enrichments and fluorescence-activated cell sorting, we demonstrated successful cultivation of Candidatus Nanohaloarchaeum antarcticus, purification of Ca. Nha. antarcticus away from other species, and growth and verification of Ca. Nha. antarcticus with Hrr. lacusprofundi these findings are analogous to those required for fulfilling Kochs postulates. We use fluorescent in situ hybridization and transmission electron microscopy to assess cell structures and interactions; metagenomics to characterize enrichment taxa, generate metagenome assembled genomes, and interrogate Antarctic communities; and proteomics to assess metabolic pathways and speculate about the roles of certain proteins. Metagenome analysis indicates the presence of a single species, which is endemic to Antarctic hypersaline systems that support the growth of haloarchaea. The presence of unusually large proteins predicted to function in attachment and invasion of hosts plus the absence of key biosynthetic pathways (e.g., lipids) in metagenome assembled genomes of globally distributed Nanohaloarchaeota indicate that all members of the lineage have evolved as symbionts. Our work expands the range of archaeal symbiotic lifestyles and provides a genetically tractable model system for advancing understanding of the factors controlling microbial symbiotic relationships. |
format |
Article in Journal/Newspaper |
author |
Hamm, JN Erdmann, S Eloe-Fadrosh, EA Angeloni, A Zhong, L Brownlee, C Williams, TJ Barton, K Carswell, S Smith, MA Brazendale, S Hancock, AM Allen, MA Raftery, MJ Cavicchioli, R |
author_facet |
Hamm, JN Erdmann, S Eloe-Fadrosh, EA Angeloni, A Zhong, L Brownlee, C Williams, TJ Barton, K Carswell, S Smith, MA Brazendale, S Hancock, AM Allen, MA Raftery, MJ Cavicchioli, R |
author_sort |
Hamm, JN |
title |
Unexpected host dependency of Antarctic Nanohaloarchaeota |
title_short |
Unexpected host dependency of Antarctic Nanohaloarchaeota |
title_full |
Unexpected host dependency of Antarctic Nanohaloarchaeota |
title_fullStr |
Unexpected host dependency of Antarctic Nanohaloarchaeota |
title_full_unstemmed |
Unexpected host dependency of Antarctic Nanohaloarchaeota |
title_sort |
unexpected host dependency of antarctic nanohaloarchaeota |
publisher |
Natl Acad Sciences |
publishDate |
2019 |
url |
https://doi.org/10.1073/pnas.1905179116 http://www.ncbi.nlm.nih.gov/pubmed/31253704 http://ecite.utas.edu.au/137594 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic antarcticus |
genre_facet |
Antarc* Antarctic antarcticus |
op_relation |
http://ecite.utas.edu.au/137594/1/137594 - Unexpected host dependency of Antarctic Nanohaloarchaeota.pdf http://dx.doi.org/10.1073/pnas.1905179116 Hamm, JN and Erdmann, S and Eloe-Fadrosh, EA and Angeloni, A and Zhong, L and Brownlee, C and Williams, TJ and Barton, K and Carswell, S and Smith, MA and Brazendale, S and Hancock, AM and Allen, MA and Raftery, MJ and Cavicchioli, R, Unexpected host dependency of Antarctic Nanohaloarchaeota, Proceedings of the National Academy of Sciences of the United States of America, 116, (29) pp. 14661-14670. ISSN 0027-8424 (2019) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/31253704 http://ecite.utas.edu.au/137594 |
op_doi |
https://doi.org/10.1073/pnas.1905179116 |
container_title |
Proceedings of the National Academy of Sciences |
container_volume |
116 |
container_issue |
29 |
container_start_page |
14661 |
op_container_end_page |
14670 |
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1766261718894247936 |