Multilocus sequence typing (MLST) and Random Polymorphic DNA (RAPD) comparisons of geographic isolates of Neoparamoeba perurans , the causative agent of Amoebic Gill Disease
Neoparamoba perurans , is the aetiological agent of amoebic gill disease (AGD), a disease that affects farmed Atlantic salmon worldwide. Multilocus sequence typing (MLST) and Random Amplified Polymorphic DNA (RAPD) are PCR-based typing methods that allow for the highly reproducible genetic analysis...
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ftunivtasecite:oai:ecite.utas.edu.au:136589 2023-05-15T15:32:30+02:00 Multilocus sequence typing (MLST) and Random Polymorphic DNA (RAPD) comparisons of geographic isolates of Neoparamoeba perurans , the causative agent of Amoebic Gill Disease Johnson-Mackinnon, JC Crosbie, PBB Karlsbakk, E Marcos-Lopez, M Paley, R Nowak, BF Bridle, AR 2019 application/pdf https://doi.org/10.3390/pathogens8040244 http://ecite.utas.edu.au/136589 en eng MDPIAG http://ecite.utas.edu.au/136589/1/136589 - Multilocus sequence typing (MLST) and Random Polymorphic DNA (RAPD) comparisons.pdf http://dx.doi.org/10.3390/pathogens8040244 Johnson-Mackinnon, JC and Crosbie, PBB and Karlsbakk, E and Marcos-Lopez, M and Paley, R and Nowak, BF and Bridle, AR, Multilocus sequence typing (MLST) and Random Polymorphic DNA (RAPD) comparisons of geographic isolates of Neoparamoeba perurans , the causative agent of Amoebic Gill Disease, Pathogens, 8, (4) Article 244. ISSN 2076-0817 (2019) [Refereed Article] http://ecite.utas.edu.au/136589 Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases Refereed Article PeerReviewed 2019 ftunivtasecite https://doi.org/10.3390/pathogens8040244 2020-03-30T22:16:18Z Neoparamoba perurans , is the aetiological agent of amoebic gill disease (AGD), a disease that affects farmed Atlantic salmon worldwide. Multilocus sequence typing (MLST) and Random Amplified Polymorphic DNA (RAPD) are PCR-based typing methods that allow for the highly reproducible genetic analysis of population structure within microbial species. To the best of our knowledge, this study represents the first use of these typing methods applied to N. perurans with the objective of distinguishing geographical isolates. These analyses were applied to a total of 16 isolates from Australia, Canada, Ireland, Scotland, Norway, and the USA. All the samples from Australia came from farm sites on the island state of Tasmania. Genetic polymorphism among isolates was more evident from the RAPD analysis compared to the MLST that used conserved housekeeping genes. Both techniques consistently identified that isolates of N. perurans from Tasmania, Australia were more similar to each other than to the isolates from other countries. While genetic differences were identified between geographical isolates, a BURST analysis provided no evidence of a founder genotype. This suggests that emerging outbreaks of AGD are not due to rapid translocation of this important salmonid pathogen from the same area. Article in Journal/Newspaper Atlantic salmon eCite UTAS (University of Tasmania) Canada Norway Pathogens 8 4 244 |
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Open Polar |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases |
spellingShingle |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases Johnson-Mackinnon, JC Crosbie, PBB Karlsbakk, E Marcos-Lopez, M Paley, R Nowak, BF Bridle, AR Multilocus sequence typing (MLST) and Random Polymorphic DNA (RAPD) comparisons of geographic isolates of Neoparamoeba perurans , the causative agent of Amoebic Gill Disease |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases |
description |
Neoparamoba perurans , is the aetiological agent of amoebic gill disease (AGD), a disease that affects farmed Atlantic salmon worldwide. Multilocus sequence typing (MLST) and Random Amplified Polymorphic DNA (RAPD) are PCR-based typing methods that allow for the highly reproducible genetic analysis of population structure within microbial species. To the best of our knowledge, this study represents the first use of these typing methods applied to N. perurans with the objective of distinguishing geographical isolates. These analyses were applied to a total of 16 isolates from Australia, Canada, Ireland, Scotland, Norway, and the USA. All the samples from Australia came from farm sites on the island state of Tasmania. Genetic polymorphism among isolates was more evident from the RAPD analysis compared to the MLST that used conserved housekeeping genes. Both techniques consistently identified that isolates of N. perurans from Tasmania, Australia were more similar to each other than to the isolates from other countries. While genetic differences were identified between geographical isolates, a BURST analysis provided no evidence of a founder genotype. This suggests that emerging outbreaks of AGD are not due to rapid translocation of this important salmonid pathogen from the same area. |
format |
Article in Journal/Newspaper |
author |
Johnson-Mackinnon, JC Crosbie, PBB Karlsbakk, E Marcos-Lopez, M Paley, R Nowak, BF Bridle, AR |
author_facet |
Johnson-Mackinnon, JC Crosbie, PBB Karlsbakk, E Marcos-Lopez, M Paley, R Nowak, BF Bridle, AR |
author_sort |
Johnson-Mackinnon, JC |
title |
Multilocus sequence typing (MLST) and Random Polymorphic DNA (RAPD) comparisons of geographic isolates of Neoparamoeba perurans , the causative agent of Amoebic Gill Disease |
title_short |
Multilocus sequence typing (MLST) and Random Polymorphic DNA (RAPD) comparisons of geographic isolates of Neoparamoeba perurans , the causative agent of Amoebic Gill Disease |
title_full |
Multilocus sequence typing (MLST) and Random Polymorphic DNA (RAPD) comparisons of geographic isolates of Neoparamoeba perurans , the causative agent of Amoebic Gill Disease |
title_fullStr |
Multilocus sequence typing (MLST) and Random Polymorphic DNA (RAPD) comparisons of geographic isolates of Neoparamoeba perurans , the causative agent of Amoebic Gill Disease |
title_full_unstemmed |
Multilocus sequence typing (MLST) and Random Polymorphic DNA (RAPD) comparisons of geographic isolates of Neoparamoeba perurans , the causative agent of Amoebic Gill Disease |
title_sort |
multilocus sequence typing (mlst) and random polymorphic dna (rapd) comparisons of geographic isolates of neoparamoeba perurans , the causative agent of amoebic gill disease |
publisher |
MDPIAG |
publishDate |
2019 |
url |
https://doi.org/10.3390/pathogens8040244 http://ecite.utas.edu.au/136589 |
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Canada Norway |
geographic_facet |
Canada Norway |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://ecite.utas.edu.au/136589/1/136589 - Multilocus sequence typing (MLST) and Random Polymorphic DNA (RAPD) comparisons.pdf http://dx.doi.org/10.3390/pathogens8040244 Johnson-Mackinnon, JC and Crosbie, PBB and Karlsbakk, E and Marcos-Lopez, M and Paley, R and Nowak, BF and Bridle, AR, Multilocus sequence typing (MLST) and Random Polymorphic DNA (RAPD) comparisons of geographic isolates of Neoparamoeba perurans , the causative agent of Amoebic Gill Disease, Pathogens, 8, (4) Article 244. ISSN 2076-0817 (2019) [Refereed Article] http://ecite.utas.edu.au/136589 |
op_doi |
https://doi.org/10.3390/pathogens8040244 |
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Pathogens |
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8 |
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4 |
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244 |
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1766362993706139648 |