Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22 : 6 omega 3)
As part of a general survey of the biodiversity and inherent ecophysiology of bacteria associated with coastal Antarctic sea-ice diatom assemblages, eight strains were identified by 16S rRNA sequence analysis as belonging to the genus Colwellia. The isolates were non-pigmented, curved rod-like cells...
Published in: | International Journal of Systematic Bacteriology |
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International union of microbiological societies
1998
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Online Access: | https://doi.org/10.1099/00207713-48-4-1171 http://ecite.utas.edu.au/13299 |
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ftunivtasecite:oai:ecite.utas.edu.au:13299 2023-05-15T13:59:07+02:00 Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22 : 6 omega 3) Bowman, JP Gosink, JJ McCammon, SA Lewis, TE Nichols, DS Nichols, PD Skerratt, J Staley, JT McMeekin, TA 1998 https://doi.org/10.1099/00207713-48-4-1171 http://ecite.utas.edu.au/13299 en eng International union of microbiological societies http://dx.doi.org/10.1099/00207713-48-4-1171 Bowman, JP and Gosink, JJ and McCammon, SA and Lewis, TE and Nichols, DS and Nichols, PD and Skerratt, J and Staley, JT and McMeekin, TA, Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22 : 6 omega 3), International Journal of Systematic Bacteriology, 48 pp. 1171-1180. ISSN 0020-7713 (1998) [Refereed Article] http://ecite.utas.edu.au/13299 Biological Sciences Microbiology Bacteriology Refereed Article PeerReviewed 1998 ftunivtasecite https://doi.org/10.1099/00207713-48-4-1171 2019-12-13T20:57:51Z As part of a general survey of the biodiversity and inherent ecophysiology of bacteria associated with coastal Antarctic sea-ice diatom assemblages, eight strains were identified by 16S rRNA sequence analysis as belonging to the genus Colwellia. The isolates were non-pigmented, curved rod-like cells which exhibited psychrophilic and facultative anaerobic growth and possessed an absolute requirement for sea water. One isolate was able to form gas vesicles. All strains synthesized the 3 polyunsaturated fatty acid (PUFA) docosahexaenoic acid (22:63, DHA) (0.7-8.0% of total fatty acids). Previously, DHA has only been detected in strains isolated from deep-sea benthic and faunal habitats and is associated with enhanced survival in permanently cold habitats. The G+C content of the DNA from the Antarctic Colwellia strains ranged from 35 to 42 mol % and DNA-DNA hybridization analyses indicated that the isolates formed five genospecies, including the species Colwellia psychrerythraea (ACAM 550(T)). 16S rRNA sequence analysis indicated that the strains formed a cluster in the -subclass of the Proteobacteria with Colwellia psychrerythraea. Sequence similarities ranged from 95.2 to 100% between the various Antarctic Colwellia isolates. Phenotypic characterization confirmed distinct differences between the different genospecies. These studies indicate that the DHA-producing Antarctic isolates consist of five different Colwellia species: Colwellia psychrerythraea and four novel species with the proposed names Colwellia demingiae sp. nov. (ACAM 459(T)), Colwellia psychrotropica sp. nov. (ACAM 179(T)), Colwellia rossensis sp. nov. (ACAM 608(T)) and Colwellia hornerae sp. nov. (ACAM 607(T)). Article in Journal/Newspaper Antarc* Antarctic Sea ice eCite UTAS (University of Tasmania) Antarctic The Antarctic International Journal of Systematic Bacteriology 48 4 1171 1180 |
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eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Biological Sciences Microbiology Bacteriology |
spellingShingle |
Biological Sciences Microbiology Bacteriology Bowman, JP Gosink, JJ McCammon, SA Lewis, TE Nichols, DS Nichols, PD Skerratt, J Staley, JT McMeekin, TA Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22 : 6 omega 3) |
topic_facet |
Biological Sciences Microbiology Bacteriology |
description |
As part of a general survey of the biodiversity and inherent ecophysiology of bacteria associated with coastal Antarctic sea-ice diatom assemblages, eight strains were identified by 16S rRNA sequence analysis as belonging to the genus Colwellia. The isolates were non-pigmented, curved rod-like cells which exhibited psychrophilic and facultative anaerobic growth and possessed an absolute requirement for sea water. One isolate was able to form gas vesicles. All strains synthesized the 3 polyunsaturated fatty acid (PUFA) docosahexaenoic acid (22:63, DHA) (0.7-8.0% of total fatty acids). Previously, DHA has only been detected in strains isolated from deep-sea benthic and faunal habitats and is associated with enhanced survival in permanently cold habitats. The G+C content of the DNA from the Antarctic Colwellia strains ranged from 35 to 42 mol % and DNA-DNA hybridization analyses indicated that the isolates formed five genospecies, including the species Colwellia psychrerythraea (ACAM 550(T)). 16S rRNA sequence analysis indicated that the strains formed a cluster in the -subclass of the Proteobacteria with Colwellia psychrerythraea. Sequence similarities ranged from 95.2 to 100% between the various Antarctic Colwellia isolates. Phenotypic characterization confirmed distinct differences between the different genospecies. These studies indicate that the DHA-producing Antarctic isolates consist of five different Colwellia species: Colwellia psychrerythraea and four novel species with the proposed names Colwellia demingiae sp. nov. (ACAM 459(T)), Colwellia psychrotropica sp. nov. (ACAM 179(T)), Colwellia rossensis sp. nov. (ACAM 608(T)) and Colwellia hornerae sp. nov. (ACAM 607(T)). |
format |
Article in Journal/Newspaper |
author |
Bowman, JP Gosink, JJ McCammon, SA Lewis, TE Nichols, DS Nichols, PD Skerratt, J Staley, JT McMeekin, TA |
author_facet |
Bowman, JP Gosink, JJ McCammon, SA Lewis, TE Nichols, DS Nichols, PD Skerratt, J Staley, JT McMeekin, TA |
author_sort |
Bowman, JP |
title |
Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22 : 6 omega 3) |
title_short |
Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22 : 6 omega 3) |
title_full |
Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22 : 6 omega 3) |
title_fullStr |
Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22 : 6 omega 3) |
title_full_unstemmed |
Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22 : 6 omega 3) |
title_sort |
colwellia demingiae sp. nov., colwellia hornerae sp. nov., colwellia rossensis sp. nov. and colwellia psychrotropica sp. nov.: psychrophilic antarctic species with the ability to synthesize docosahexaenoic acid (22 : 6 omega 3) |
publisher |
International union of microbiological societies |
publishDate |
1998 |
url |
https://doi.org/10.1099/00207713-48-4-1171 http://ecite.utas.edu.au/13299 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Sea ice |
genre_facet |
Antarc* Antarctic Sea ice |
op_relation |
http://dx.doi.org/10.1099/00207713-48-4-1171 Bowman, JP and Gosink, JJ and McCammon, SA and Lewis, TE and Nichols, DS and Nichols, PD and Skerratt, J and Staley, JT and McMeekin, TA, Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22 : 6 omega 3), International Journal of Systematic Bacteriology, 48 pp. 1171-1180. ISSN 0020-7713 (1998) [Refereed Article] http://ecite.utas.edu.au/13299 |
op_doi |
https://doi.org/10.1099/00207713-48-4-1171 |
container_title |
International Journal of Systematic Bacteriology |
container_volume |
48 |
container_issue |
4 |
container_start_page |
1171 |
op_container_end_page |
1180 |
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1766267490016428032 |