Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods?
Stable isotopes analysis (SIA) is a powerful tool to estimate dietary links between polar zooplankton. However, the presence of highly variable 12 C‐rich lipids may skew estimations as they are depleted in 13 C relative to proteins and carbohydrates, consequently masking carbon signals from food sou...
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Online Access: | https://doi.org/10.1002/rcm.8384 http://www.ncbi.nlm.nih.gov/pubmed/30635929 http://ecite.utas.edu.au/130393 |
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ftunivtasecite:oai:ecite.utas.edu.au:130393 2023-05-15T13:55:18+02:00 Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods? Weldrick, CK Trebilco, R Swadling, KM 2019 https://doi.org/10.1002/rcm.8384 http://www.ncbi.nlm.nih.gov/pubmed/30635929 http://ecite.utas.edu.au/130393 en eng John Wiley & Sons Ltd http://dx.doi.org/10.1002/rcm.8384 Weldrick, CK and Trebilco, R and Swadling, KM, Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods?, Rapid Communications in Mass Spectrometry, 33, (6) pp. 569-578. ISSN 0951-4198 (2019) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/30635929 http://ecite.utas.edu.au/130393 Biological Sciences Ecology Community Ecology Refereed Article PeerReviewed 2019 ftunivtasecite https://doi.org/10.1002/rcm.8384 2020-01-06T23:16:17Z Stable isotopes analysis (SIA) is a powerful tool to estimate dietary links between polar zooplankton. However, the presence of highly variable 12 C‐rich lipids may skew estimations as they are depleted in 13 C relative to proteins and carbohydrates, consequently masking carbon signals from food sources. Lipid effects on pteropod‐specific values requires examining, since accounting for lipids is rarely conducted among the few existing pteropod‐related SIA studies. It is currently unclear whether lipid correction is necessary prior to SIA of pteropods. Methods Whole bodies of three species of pteropods ( Clio pyramidata f. sulcata , Clione limacina antarctica , and Spongiobranchaea australis ) sampled from the Southern Ocean were lipid‐extracted chemically to test the effects on δ 13 C and δ 15 N values (n=38 individuals in total). We determined the average change in δ 13 C values for each treatment, and compared this offset with those from published normalization models. We tested lipid correction effects on isotopic niche dispersion metrics to compare interpretations surrounding food web dynamics. Results Pteropods with lipids removed had δ 13 C values up to 4.5 higher than bulk samples. However, lipid extraction also produced higher δ 15 N values than bulk samples. Isotopic niche overlaps between untreated pteropods and their potential food sources were significantly different from overlaps generated between lipid‐corrected pteropods and their potential food sources. Data converted using several published normalization models did not reveal significant differences among various calculated niche metrics, including standard ellipse and total area. Conclusions We recommend accounting for lipids via chemical extraction or mathematical normalization before applying SIA to calculate ecological niche metrics, particularly for organisms with moderate to high lipid content such as polar pteropods. Failure to account for lipids may result in misinterpretations of niche dimensions and overlap and, consequently, trophic interactions. Article in Journal/Newspaper Antarc* Antarctica Clione limacina Limacina antarctica Southern Ocean eCite UTAS (University of Tasmania) Southern Ocean Rapid Communications in Mass Spectrometry 33 6 569 578 |
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Open Polar |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Biological Sciences Ecology Community Ecology |
spellingShingle |
Biological Sciences Ecology Community Ecology Weldrick, CK Trebilco, R Swadling, KM Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods? |
topic_facet |
Biological Sciences Ecology Community Ecology |
description |
Stable isotopes analysis (SIA) is a powerful tool to estimate dietary links between polar zooplankton. However, the presence of highly variable 12 C‐rich lipids may skew estimations as they are depleted in 13 C relative to proteins and carbohydrates, consequently masking carbon signals from food sources. Lipid effects on pteropod‐specific values requires examining, since accounting for lipids is rarely conducted among the few existing pteropod‐related SIA studies. It is currently unclear whether lipid correction is necessary prior to SIA of pteropods. Methods Whole bodies of three species of pteropods ( Clio pyramidata f. sulcata , Clione limacina antarctica , and Spongiobranchaea australis ) sampled from the Southern Ocean were lipid‐extracted chemically to test the effects on δ 13 C and δ 15 N values (n=38 individuals in total). We determined the average change in δ 13 C values for each treatment, and compared this offset with those from published normalization models. We tested lipid correction effects on isotopic niche dispersion metrics to compare interpretations surrounding food web dynamics. Results Pteropods with lipids removed had δ 13 C values up to 4.5 higher than bulk samples. However, lipid extraction also produced higher δ 15 N values than bulk samples. Isotopic niche overlaps between untreated pteropods and their potential food sources were significantly different from overlaps generated between lipid‐corrected pteropods and their potential food sources. Data converted using several published normalization models did not reveal significant differences among various calculated niche metrics, including standard ellipse and total area. Conclusions We recommend accounting for lipids via chemical extraction or mathematical normalization before applying SIA to calculate ecological niche metrics, particularly for organisms with moderate to high lipid content such as polar pteropods. Failure to account for lipids may result in misinterpretations of niche dimensions and overlap and, consequently, trophic interactions. |
format |
Article in Journal/Newspaper |
author |
Weldrick, CK Trebilco, R Swadling, KM |
author_facet |
Weldrick, CK Trebilco, R Swadling, KM |
author_sort |
Weldrick, CK |
title |
Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods? |
title_short |
Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods? |
title_full |
Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods? |
title_fullStr |
Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods? |
title_full_unstemmed |
Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods? |
title_sort |
can lipid removal affect interpretation of resource partitioning from stable isotopes in southern ocean pteropods? |
publisher |
John Wiley & Sons Ltd |
publishDate |
2019 |
url |
https://doi.org/10.1002/rcm.8384 http://www.ncbi.nlm.nih.gov/pubmed/30635929 http://ecite.utas.edu.au/130393 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Antarc* Antarctica Clione limacina Limacina antarctica Southern Ocean |
genre_facet |
Antarc* Antarctica Clione limacina Limacina antarctica Southern Ocean |
op_relation |
http://dx.doi.org/10.1002/rcm.8384 Weldrick, CK and Trebilco, R and Swadling, KM, Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods?, Rapid Communications in Mass Spectrometry, 33, (6) pp. 569-578. ISSN 0951-4198 (2019) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/30635929 http://ecite.utas.edu.au/130393 |
op_doi |
https://doi.org/10.1002/rcm.8384 |
container_title |
Rapid Communications in Mass Spectrometry |
container_volume |
33 |
container_issue |
6 |
container_start_page |
569 |
op_container_end_page |
578 |
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1766261710687043584 |