Methanotrophic bacteria in oilsands tailings ponds of northern Alberta
We investigated methanotrophic bacteria in slightly alkaline surface water (pH 7.48.7) of oilsands tailings ponds in Fort McMurray, Canada. These large lakes (up to 10 km 2 ) contain water, silt, clay and residual hydrocarbons that are not recovered in oilsands mining. They are primarily anoxic and...
Published in: | The ISME Journal |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Nature Publishing Group
2013
|
Subjects: | |
Online Access: | https://doi.org/10.1038/ismej.2012.163 http://www.ncbi.nlm.nih.gov/pubmed/23254511 http://ecite.utas.edu.au/116638 |
id |
ftunivtasecite:oai:ecite.utas.edu.au:116638 |
---|---|
record_format |
openpolar |
spelling |
ftunivtasecite:oai:ecite.utas.edu.au:116638 2023-05-15T16:17:39+02:00 Methanotrophic bacteria in oilsands tailings ponds of northern Alberta Saidi-Mehrabad, A He, Z Tamas, I Sharp, CE Brady, AL Rochman, FF Bodrossy, L Abell, GCJ Penner, T Dong, X Sensen, CW Dunfield, PF 2013 https://doi.org/10.1038/ismej.2012.163 http://www.ncbi.nlm.nih.gov/pubmed/23254511 http://ecite.utas.edu.au/116638 en eng Nature Publishing Group http://dx.doi.org/10.1038/ismej.2012.163 Saidi-Mehrabad, A and He, Z and Tamas, I and Sharp, CE and Brady, AL and Rochman, FF and Bodrossy, L and Abell, GCJ and Penner, T and Dong, X and Sensen, CW and Dunfield, PF, Methanotrophic bacteria in oilsands tailings ponds of northern Alberta, ISME Journal: multidisciplinary journal of microbial ecology, 7 pp. 908-921. ISSN 1751-7362 (2013) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/23254511 http://ecite.utas.edu.au/116638 Biological Sciences Ecology Community Ecology Refereed Article PeerReviewed 2013 ftunivtasecite https://doi.org/10.1038/ismej.2012.163 2019-12-13T22:16:11Z We investigated methanotrophic bacteria in slightly alkaline surface water (pH 7.48.7) of oilsands tailings ponds in Fort McMurray, Canada. These large lakes (up to 10 km 2 ) contain water, silt, clay and residual hydrocarbons that are not recovered in oilsands mining. They are primarily anoxic and produce methane but have an aerobic surface layer. Aerobic methane oxidation was measured in the surface water at rates up to 152 nmol CH 4 ml −1 water d −1 . Microbial diversity was investigated via pyrotag sequencing of amplified 16S rRNA genes, as well as by analysis of methanotroph-specific pmoA genes using both pyrosequencing and microarray analysis. The predominantly detected methanotroph in surface waters at all sampling times was an uncultured species related to the gammaproteobacterial genus Methylocaldum , although a few other methanotrophs were also detected, including Methylomonas spp. Active species were identified via 13 CH 4 stable isotope probing (SIP) of DNA, combined with pyrotag sequencing and shotgun metagenomic sequencing of heavy 13 C-DNA. The SIP-PCR results demonstrated that the Methylocaldum and Methylomonas spp. actively consumed methane in fresh tailings pond water. Metagenomic analysis of DNA from the heavy SIP fraction verified the PCR-based results and identified additional pmoA genes not detected via PCR. The metagenome indicated that the overall methylotrophic community possessed known pathways for formaldehyde oxidation, carbon fixation and detoxification of nitrogenous compounds but appeared to possess only particulate methane monooxygenase not soluble methane monooxygenase. Article in Journal/Newspaper Fort McMurray eCite UTAS (University of Tasmania) Canada Fort McMurray The ISME Journal 7 5 908 921 |
institution |
Open Polar |
collection |
eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Biological Sciences Ecology Community Ecology |
spellingShingle |
Biological Sciences Ecology Community Ecology Saidi-Mehrabad, A He, Z Tamas, I Sharp, CE Brady, AL Rochman, FF Bodrossy, L Abell, GCJ Penner, T Dong, X Sensen, CW Dunfield, PF Methanotrophic bacteria in oilsands tailings ponds of northern Alberta |
topic_facet |
Biological Sciences Ecology Community Ecology |
description |
We investigated methanotrophic bacteria in slightly alkaline surface water (pH 7.48.7) of oilsands tailings ponds in Fort McMurray, Canada. These large lakes (up to 10 km 2 ) contain water, silt, clay and residual hydrocarbons that are not recovered in oilsands mining. They are primarily anoxic and produce methane but have an aerobic surface layer. Aerobic methane oxidation was measured in the surface water at rates up to 152 nmol CH 4 ml −1 water d −1 . Microbial diversity was investigated via pyrotag sequencing of amplified 16S rRNA genes, as well as by analysis of methanotroph-specific pmoA genes using both pyrosequencing and microarray analysis. The predominantly detected methanotroph in surface waters at all sampling times was an uncultured species related to the gammaproteobacterial genus Methylocaldum , although a few other methanotrophs were also detected, including Methylomonas spp. Active species were identified via 13 CH 4 stable isotope probing (SIP) of DNA, combined with pyrotag sequencing and shotgun metagenomic sequencing of heavy 13 C-DNA. The SIP-PCR results demonstrated that the Methylocaldum and Methylomonas spp. actively consumed methane in fresh tailings pond water. Metagenomic analysis of DNA from the heavy SIP fraction verified the PCR-based results and identified additional pmoA genes not detected via PCR. The metagenome indicated that the overall methylotrophic community possessed known pathways for formaldehyde oxidation, carbon fixation and detoxification of nitrogenous compounds but appeared to possess only particulate methane monooxygenase not soluble methane monooxygenase. |
format |
Article in Journal/Newspaper |
author |
Saidi-Mehrabad, A He, Z Tamas, I Sharp, CE Brady, AL Rochman, FF Bodrossy, L Abell, GCJ Penner, T Dong, X Sensen, CW Dunfield, PF |
author_facet |
Saidi-Mehrabad, A He, Z Tamas, I Sharp, CE Brady, AL Rochman, FF Bodrossy, L Abell, GCJ Penner, T Dong, X Sensen, CW Dunfield, PF |
author_sort |
Saidi-Mehrabad, A |
title |
Methanotrophic bacteria in oilsands tailings ponds of northern Alberta |
title_short |
Methanotrophic bacteria in oilsands tailings ponds of northern Alberta |
title_full |
Methanotrophic bacteria in oilsands tailings ponds of northern Alberta |
title_fullStr |
Methanotrophic bacteria in oilsands tailings ponds of northern Alberta |
title_full_unstemmed |
Methanotrophic bacteria in oilsands tailings ponds of northern Alberta |
title_sort |
methanotrophic bacteria in oilsands tailings ponds of northern alberta |
publisher |
Nature Publishing Group |
publishDate |
2013 |
url |
https://doi.org/10.1038/ismej.2012.163 http://www.ncbi.nlm.nih.gov/pubmed/23254511 http://ecite.utas.edu.au/116638 |
geographic |
Canada Fort McMurray |
geographic_facet |
Canada Fort McMurray |
genre |
Fort McMurray |
genre_facet |
Fort McMurray |
op_relation |
http://dx.doi.org/10.1038/ismej.2012.163 Saidi-Mehrabad, A and He, Z and Tamas, I and Sharp, CE and Brady, AL and Rochman, FF and Bodrossy, L and Abell, GCJ and Penner, T and Dong, X and Sensen, CW and Dunfield, PF, Methanotrophic bacteria in oilsands tailings ponds of northern Alberta, ISME Journal: multidisciplinary journal of microbial ecology, 7 pp. 908-921. ISSN 1751-7362 (2013) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/23254511 http://ecite.utas.edu.au/116638 |
op_doi |
https://doi.org/10.1038/ismej.2012.163 |
container_title |
The ISME Journal |
container_volume |
7 |
container_issue |
5 |
container_start_page |
908 |
op_container_end_page |
921 |
_version_ |
1766003548276916224 |