A primer to metabarcoding surveys of Antarctic terrestrial biodiversity
Ice-free regions of Antarctica are concentrated along the coastal margins but are scarce throughout the continental interior. Environmental changes, including the introduction of non-indigenous species, increasingly threaten these unique habitats. At the same time, the unique biotic communities subs...
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Online Access: | https://doi.org/10.1017/S0954102016000389 http://ecite.utas.edu.au/114583 |
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ftunivtasecite:oai:ecite.utas.edu.au:114583 2023-05-15T13:49:03+02:00 A primer to metabarcoding surveys of Antarctic terrestrial biodiversity Czechowski, P Clarke, LJ Cooper, A Stevens, MI 2017 https://doi.org/10.1017/S0954102016000389 http://ecite.utas.edu.au/114583 en eng Cambridge Univ Press http://dx.doi.org/10.1017/S0954102016000389 Czechowski, P and Clarke, LJ and Cooper, A and Stevens, MI, A primer to metabarcoding surveys of Antarctic terrestrial biodiversity, Antarctic Science, 29, (1) pp. 3-15. ISSN 0954-1020 (2017) [Refereed Article] http://ecite.utas.edu.au/114583 Biological Sciences Microbiology Microbial Ecology Refereed Article PeerReviewed 2017 ftunivtasecite https://doi.org/10.1017/S0954102016000389 2019-12-13T22:14:30Z Ice-free regions of Antarctica are concentrated along the coastal margins but are scarce throughout the continental interior. Environmental changes, including the introduction of non-indigenous species, increasingly threaten these unique habitats. At the same time, the unique biotic communities subsisting in isolation across the continent are difficult to survey due to logistical constraints, sampling challenges and problems related to the identification of small and cryptic taxa. Baseline biodiversity data from remote Antarctic habitats are still missing for many parts of the continent but are critical to the detection of community changes over time, including newly introduced species. Here we review the potential of standardized (non-specialist) sampling in the field (e.g. from soil, vegetation or water) combined with high-throughput sequencing (HTS) of bulk DNA as a possible solution to overcome some of these problems. In particular, HTS metabarcoding approaches benefit from being able to process many samples in parallel, while workflow and data structure can stay highly uniform. Such approaches have quickly gained recognition and we show that HTS metabarcoding surveys are likely to play an important role in continent-wide biomonitoring of all Antarctic terrestrial habitats. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Antarctica eCite UTAS (University of Tasmania) Antarctic Antarctic Science 29 1 3 15 |
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Open Polar |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Biological Sciences Microbiology Microbial Ecology |
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Biological Sciences Microbiology Microbial Ecology Czechowski, P Clarke, LJ Cooper, A Stevens, MI A primer to metabarcoding surveys of Antarctic terrestrial biodiversity |
topic_facet |
Biological Sciences Microbiology Microbial Ecology |
description |
Ice-free regions of Antarctica are concentrated along the coastal margins but are scarce throughout the continental interior. Environmental changes, including the introduction of non-indigenous species, increasingly threaten these unique habitats. At the same time, the unique biotic communities subsisting in isolation across the continent are difficult to survey due to logistical constraints, sampling challenges and problems related to the identification of small and cryptic taxa. Baseline biodiversity data from remote Antarctic habitats are still missing for many parts of the continent but are critical to the detection of community changes over time, including newly introduced species. Here we review the potential of standardized (non-specialist) sampling in the field (e.g. from soil, vegetation or water) combined with high-throughput sequencing (HTS) of bulk DNA as a possible solution to overcome some of these problems. In particular, HTS metabarcoding approaches benefit from being able to process many samples in parallel, while workflow and data structure can stay highly uniform. Such approaches have quickly gained recognition and we show that HTS metabarcoding surveys are likely to play an important role in continent-wide biomonitoring of all Antarctic terrestrial habitats. |
format |
Article in Journal/Newspaper |
author |
Czechowski, P Clarke, LJ Cooper, A Stevens, MI |
author_facet |
Czechowski, P Clarke, LJ Cooper, A Stevens, MI |
author_sort |
Czechowski, P |
title |
A primer to metabarcoding surveys of Antarctic terrestrial biodiversity |
title_short |
A primer to metabarcoding surveys of Antarctic terrestrial biodiversity |
title_full |
A primer to metabarcoding surveys of Antarctic terrestrial biodiversity |
title_fullStr |
A primer to metabarcoding surveys of Antarctic terrestrial biodiversity |
title_full_unstemmed |
A primer to metabarcoding surveys of Antarctic terrestrial biodiversity |
title_sort |
primer to metabarcoding surveys of antarctic terrestrial biodiversity |
publisher |
Cambridge Univ Press |
publishDate |
2017 |
url |
https://doi.org/10.1017/S0954102016000389 http://ecite.utas.edu.au/114583 |
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Antarctic |
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Antarctic |
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Antarc* Antarctic Antarctic Science Antarctica |
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Antarc* Antarctic Antarctic Science Antarctica |
op_relation |
http://dx.doi.org/10.1017/S0954102016000389 Czechowski, P and Clarke, LJ and Cooper, A and Stevens, MI, A primer to metabarcoding surveys of Antarctic terrestrial biodiversity, Antarctic Science, 29, (1) pp. 3-15. ISSN 0954-1020 (2017) [Refereed Article] http://ecite.utas.edu.au/114583 |
op_doi |
https://doi.org/10.1017/S0954102016000389 |
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Antarctic Science |
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29 |
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1 |
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3 |
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15 |
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1766250688417890304 |