Preliminary validation of a high docosahexaenoic acid (DHA) and -linolenic acid (ALA) dietary oil blend: tissue fatty acid composition and liver proteome response in Atlantic salmon ( Salmo salar ) smolts
Marine oils are important to human nutrition as the major source of docosahexaenoic acid (DHA), a key omega-3 long-chain (≥C 20 ) polyunsaturated fatty acid (n-3 LC-PUFA) that is low or lacking in terrestrial plant or animal oils. The inclusion of fish oil as main source of n-3 LC-PUFA in aquafeeds...
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Online Access: | https://doi.org/10.1371/journal.pone.0161513 http://www.ncbi.nlm.nih.gov/pubmed/27556399 http://ecite.utas.edu.au/111025 |
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ftunivtasecite:oai:ecite.utas.edu.au:111025 2023-05-15T15:31:29+02:00 Preliminary validation of a high docosahexaenoic acid (DHA) and -linolenic acid (ALA) dietary oil blend: tissue fatty acid composition and liver proteome response in Atlantic salmon ( Salmo salar ) smolts Nuez-Ortin, WG Carter, CG Wilson, R Cooke, I Nichols, PD 2016 application/pdf https://doi.org/10.1371/journal.pone.0161513 http://www.ncbi.nlm.nih.gov/pubmed/27556399 http://ecite.utas.edu.au/111025 en eng Public Library of Science http://ecite.utas.edu.au/111025/1/journal.pone.0161513_Oil MS.pdf http://dx.doi.org/10.1371/journal.pone.0161513 Nuez-Ortin, WG and Carter, CG and Wilson, R and Cooke, I and Nichols, PD, Preliminary validation of a high docosahexaenoic acid (DHA) and -linolenic acid (ALA) dietary oil blend: tissue fatty acid composition and liver proteome response in Atlantic salmon ( Salmo salar ) smolts, PLoS One, 13, (5) Article e0197800. ISSN 1932-6203 (2016) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/27556399 http://ecite.utas.edu.au/111025 Biological Sciences Biochemistry and cell biology Proteomics and intermolecular interactions (excl. medical proteomics) Refereed Article PeerReviewed 2016 ftunivtasecite https://doi.org/10.1371/journal.pone.0161513 2021-02-22T23:16:29Z Marine oils are important to human nutrition as the major source of docosahexaenoic acid (DHA), a key omega-3 long-chain (≥C 20 ) polyunsaturated fatty acid (n-3 LC-PUFA) that is low or lacking in terrestrial plant or animal oils. The inclusion of fish oil as main source of n-3 LC-PUFA in aquafeeds is mostly limited by the increasing price and decreasing availability. Fish oil replacement with cheaper terrestrial plant and animal oils has considerably reduced the content of n-3 LC-PUFA in flesh of farmed Atlantic salmon. Novel DHA-enriched oils with high alpha-linolenic acid (ALA) content will be available from transgenic oilseeds plants in the near future as an alternative for dietary fish oil replacement in aquafeeds. As a preliminary validation, we formulated an oil blend (TOFX) with high DHA and ALA content using tuna oil (TO) high in DHA and the flaxseed oil (FX) high in ALA, and assessed its ability to achieve fish oil-like n-3 LC-PUFA tissue composition in Atlantic salmon smolts. We applied proteomics as an exploratory approach to understand the effects of nutritional changes on the fish liver. Comparisons were made between fish fed a fish oil-based diet (FO) and a commercial-like oil blend diet (fish oil + poultry oil, FOPO) over 89 days. Growth and feed efficiency ratio were lower on the TOFX diet. Fish muscle concentration of n-3 LC-PUFA was significantly higher for TOFX than for FOPO fish, but not higher than for FO fish, while retention efficiency of n-3 LC-PUFA was promoted by TOFX relative to FO. Proteomics analysis revealed an oxidative stress response indicative of the main adaptive physiological mechanism in TOFX fish. While specific dietary fatty acid concentrations and balances and antioxidant supplementation may need further attention, the use of an oil with a high content of DHA and ALA can enhance tissue deposition of n-3 LC-PUFA in relation to a commercially used oil blend. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) PLOS ONE 11 8 e0161513 |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Biological Sciences Biochemistry and cell biology Proteomics and intermolecular interactions (excl. medical proteomics) |
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Biological Sciences Biochemistry and cell biology Proteomics and intermolecular interactions (excl. medical proteomics) Nuez-Ortin, WG Carter, CG Wilson, R Cooke, I Nichols, PD Preliminary validation of a high docosahexaenoic acid (DHA) and -linolenic acid (ALA) dietary oil blend: tissue fatty acid composition and liver proteome response in Atlantic salmon ( Salmo salar ) smolts |
topic_facet |
Biological Sciences Biochemistry and cell biology Proteomics and intermolecular interactions (excl. medical proteomics) |
description |
Marine oils are important to human nutrition as the major source of docosahexaenoic acid (DHA), a key omega-3 long-chain (≥C 20 ) polyunsaturated fatty acid (n-3 LC-PUFA) that is low or lacking in terrestrial plant or animal oils. The inclusion of fish oil as main source of n-3 LC-PUFA in aquafeeds is mostly limited by the increasing price and decreasing availability. Fish oil replacement with cheaper terrestrial plant and animal oils has considerably reduced the content of n-3 LC-PUFA in flesh of farmed Atlantic salmon. Novel DHA-enriched oils with high alpha-linolenic acid (ALA) content will be available from transgenic oilseeds plants in the near future as an alternative for dietary fish oil replacement in aquafeeds. As a preliminary validation, we formulated an oil blend (TOFX) with high DHA and ALA content using tuna oil (TO) high in DHA and the flaxseed oil (FX) high in ALA, and assessed its ability to achieve fish oil-like n-3 LC-PUFA tissue composition in Atlantic salmon smolts. We applied proteomics as an exploratory approach to understand the effects of nutritional changes on the fish liver. Comparisons were made between fish fed a fish oil-based diet (FO) and a commercial-like oil blend diet (fish oil + poultry oil, FOPO) over 89 days. Growth and feed efficiency ratio were lower on the TOFX diet. Fish muscle concentration of n-3 LC-PUFA was significantly higher for TOFX than for FOPO fish, but not higher than for FO fish, while retention efficiency of n-3 LC-PUFA was promoted by TOFX relative to FO. Proteomics analysis revealed an oxidative stress response indicative of the main adaptive physiological mechanism in TOFX fish. While specific dietary fatty acid concentrations and balances and antioxidant supplementation may need further attention, the use of an oil with a high content of DHA and ALA can enhance tissue deposition of n-3 LC-PUFA in relation to a commercially used oil blend. |
format |
Article in Journal/Newspaper |
author |
Nuez-Ortin, WG Carter, CG Wilson, R Cooke, I Nichols, PD |
author_facet |
Nuez-Ortin, WG Carter, CG Wilson, R Cooke, I Nichols, PD |
author_sort |
Nuez-Ortin, WG |
title |
Preliminary validation of a high docosahexaenoic acid (DHA) and -linolenic acid (ALA) dietary oil blend: tissue fatty acid composition and liver proteome response in Atlantic salmon ( Salmo salar ) smolts |
title_short |
Preliminary validation of a high docosahexaenoic acid (DHA) and -linolenic acid (ALA) dietary oil blend: tissue fatty acid composition and liver proteome response in Atlantic salmon ( Salmo salar ) smolts |
title_full |
Preliminary validation of a high docosahexaenoic acid (DHA) and -linolenic acid (ALA) dietary oil blend: tissue fatty acid composition and liver proteome response in Atlantic salmon ( Salmo salar ) smolts |
title_fullStr |
Preliminary validation of a high docosahexaenoic acid (DHA) and -linolenic acid (ALA) dietary oil blend: tissue fatty acid composition and liver proteome response in Atlantic salmon ( Salmo salar ) smolts |
title_full_unstemmed |
Preliminary validation of a high docosahexaenoic acid (DHA) and -linolenic acid (ALA) dietary oil blend: tissue fatty acid composition and liver proteome response in Atlantic salmon ( Salmo salar ) smolts |
title_sort |
preliminary validation of a high docosahexaenoic acid (dha) and -linolenic acid (ala) dietary oil blend: tissue fatty acid composition and liver proteome response in atlantic salmon ( salmo salar ) smolts |
publisher |
Public Library of Science |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pone.0161513 http://www.ncbi.nlm.nih.gov/pubmed/27556399 http://ecite.utas.edu.au/111025 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://ecite.utas.edu.au/111025/1/journal.pone.0161513_Oil MS.pdf http://dx.doi.org/10.1371/journal.pone.0161513 Nuez-Ortin, WG and Carter, CG and Wilson, R and Cooke, I and Nichols, PD, Preliminary validation of a high docosahexaenoic acid (DHA) and -linolenic acid (ALA) dietary oil blend: tissue fatty acid composition and liver proteome response in Atlantic salmon ( Salmo salar ) smolts, PLoS One, 13, (5) Article e0197800. ISSN 1932-6203 (2016) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/27556399 http://ecite.utas.edu.au/111025 |
op_doi |
https://doi.org/10.1371/journal.pone.0161513 |
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PLOS ONE |
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11 |
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e0161513 |
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