Effects of anaesthetics containing eugenol on Neoparamoeba perurans
Oil extracted from cloves, Syzygium aromaticum , consists mainly of eugenol, which has been documented to affect protozoa attachment, viability and growth. Isoeugenol (sold under the trade name AQUI-S ) is a commercially available fish anaesthetic produced by isomerisation of eugenol. Both are anaes...
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Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier Science Bv
2016
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Online Access: | https://doi.org/10.1016/j.aquaculture.2016.05.033 http://ecite.utas.edu.au/110234 |
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ftunivtasecite:oai:ecite.utas.edu.au:110234 2023-05-15T15:32:31+02:00 Effects of anaesthetics containing eugenol on Neoparamoeba perurans Shijie, L Adams, MB Nowak, BF Crosbie, PBB 2016 https://doi.org/10.1016/j.aquaculture.2016.05.033 http://ecite.utas.edu.au/110234 en eng Elsevier Science Bv http://dx.doi.org/10.1016/j.aquaculture.2016.05.033 Shijie, L and Adams, MB and Nowak, BF and Crosbie, PBB, Effects of anaesthetics containing eugenol on Neoparamoeba perurans , Aquaculture, 463 pp. 159-162. ISSN 0044-8486 (2016) [Refereed Article] http://ecite.utas.edu.au/110234 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Refereed Article PeerReviewed 2016 ftunivtasecite https://doi.org/10.1016/j.aquaculture.2016.05.033 2019-12-13T22:10:45Z Oil extracted from cloves, Syzygium aromaticum , consists mainly of eugenol, which has been documented to affect protozoa attachment, viability and growth. Isoeugenol (sold under the trade name AQUI-S ) is a commercially available fish anaesthetic produced by isomerisation of eugenol. Both are anaesthetics commonly employed during the husbandry and euthanasia of Atlantic salmon, Salmo salar L., used for amoebic gill disease (AGD) research. Neoparamoeba perurans (the causative agent of AGD) trophozoites were exposed to clove oil at 10, 20, 40 and 80μLL −1 for 10min and 5, 10, 20 and 40μLL −1 for 120min; AQUI-S at 5, 10, 20 and 40μLL −1 for 10min and 2.5, 5, 10 and 20μLL −1 for 120min There were no significant differences in viability and survival of trophozoites after exposure to the anaesthetics. When trophozoites were exposed to clove oil at 80μLL −1 for 10min there was significantly ( P <0.05) more detachment from a plastic surface compared to the control. Continued use of clove oil and AQUI-S at 40μLL −1 or less for up to 120min is unlikely to have a detrimental impact on amoebae that are isolated and collected from salmon with AGD to be used for downstream research such as projects involving disease challenges and in vitro screening of anti- N. perurans compounds. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Aquaculture 463 159 162 |
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Open Polar |
collection |
eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
spellingShingle |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Shijie, L Adams, MB Nowak, BF Crosbie, PBB Effects of anaesthetics containing eugenol on Neoparamoeba perurans |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
description |
Oil extracted from cloves, Syzygium aromaticum , consists mainly of eugenol, which has been documented to affect protozoa attachment, viability and growth. Isoeugenol (sold under the trade name AQUI-S ) is a commercially available fish anaesthetic produced by isomerisation of eugenol. Both are anaesthetics commonly employed during the husbandry and euthanasia of Atlantic salmon, Salmo salar L., used for amoebic gill disease (AGD) research. Neoparamoeba perurans (the causative agent of AGD) trophozoites were exposed to clove oil at 10, 20, 40 and 80μLL −1 for 10min and 5, 10, 20 and 40μLL −1 for 120min; AQUI-S at 5, 10, 20 and 40μLL −1 for 10min and 2.5, 5, 10 and 20μLL −1 for 120min There were no significant differences in viability and survival of trophozoites after exposure to the anaesthetics. When trophozoites were exposed to clove oil at 80μLL −1 for 10min there was significantly ( P <0.05) more detachment from a plastic surface compared to the control. Continued use of clove oil and AQUI-S at 40μLL −1 or less for up to 120min is unlikely to have a detrimental impact on amoebae that are isolated and collected from salmon with AGD to be used for downstream research such as projects involving disease challenges and in vitro screening of anti- N. perurans compounds. |
format |
Article in Journal/Newspaper |
author |
Shijie, L Adams, MB Nowak, BF Crosbie, PBB |
author_facet |
Shijie, L Adams, MB Nowak, BF Crosbie, PBB |
author_sort |
Shijie, L |
title |
Effects of anaesthetics containing eugenol on Neoparamoeba perurans |
title_short |
Effects of anaesthetics containing eugenol on Neoparamoeba perurans |
title_full |
Effects of anaesthetics containing eugenol on Neoparamoeba perurans |
title_fullStr |
Effects of anaesthetics containing eugenol on Neoparamoeba perurans |
title_full_unstemmed |
Effects of anaesthetics containing eugenol on Neoparamoeba perurans |
title_sort |
effects of anaesthetics containing eugenol on neoparamoeba perurans |
publisher |
Elsevier Science Bv |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.aquaculture.2016.05.033 http://ecite.utas.edu.au/110234 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://dx.doi.org/10.1016/j.aquaculture.2016.05.033 Shijie, L and Adams, MB and Nowak, BF and Crosbie, PBB, Effects of anaesthetics containing eugenol on Neoparamoeba perurans , Aquaculture, 463 pp. 159-162. ISSN 0044-8486 (2016) [Refereed Article] http://ecite.utas.edu.au/110234 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2016.05.033 |
container_title |
Aquaculture |
container_volume |
463 |
container_start_page |
159 |
op_container_end_page |
162 |
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1766363012102356992 |