Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish
Hemoglobin concentration [Hb] is measured for a wide variety of animal studies. The use of Point-of-care devices, such as the HemoCue, is becoming increasingly common due to their portability, relative ease of use and low cost. This study aimed to determine whether the [Hb] of blue-tongued skink ( T...
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Online Access: | https://doi.org/10.1093/conphys/cow006 http://ecite.utas.edu.au/106982 |
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ftunivtasecite:oai:ecite.utas.edu.au:106982 2023-05-15T15:31:00+02:00 Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish Andrewartha, SJ Munns, SL Edwards, A 2016 application/pdf https://doi.org/10.1093/conphys/cow006 http://ecite.utas.edu.au/106982 en eng Oxford University Press http://ecite.utas.edu.au/106982/1/Conserv Physiol-2016-Andrewartha.pdf http://dx.doi.org/10.1093/conphys/cow006 Andrewartha, SJ and Munns, SL and Edwards, A, Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish, Conservation Physiology, 4, (1) pp. 1-6. ISSN 2051-1434 (2016) [Refereed Article] http://ecite.utas.edu.au/106982 Biological Sciences Other Biological Sciences Biological Sciences not elsewhere classified Refereed Article PeerReviewed 2016 ftunivtasecite https://doi.org/10.1093/conphys/cow006 2019-12-13T22:07:59Z Hemoglobin concentration [Hb] is measured for a wide variety of animal studies. The use of Point-of-care devices, such as the HemoCue, is becoming increasingly common due to their portability, relative ease of use and low cost. This study aimed to determine whether the [Hb] of blue-tongued skink ( Tiliqua nigrolutea ) blood can be accurately determined using the HemoCue and whether the HemoCue overestimates [Hb] of reptile blood in a similar manner to fish blood. We additionally aimed to test whether ploidy affected [Hb] determined by the HemoCue using blood from diploid and triploid Atlantic salmon ( Salmo salar ). The HemoCue Hb 201 + systematically overestimated [Hb] in both blue-tongued skinks and Atlantic salmon and there was no difference between calibration equations determined for diploid or triploid salmon. The overestimation was systematic in both species, and as such, [Hb] determined by the HemoCue can be corrected using appropriate calibration equations. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Conservation Physiology 4 1 cow006 |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Biological Sciences Other Biological Sciences Biological Sciences not elsewhere classified |
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Biological Sciences Other Biological Sciences Biological Sciences not elsewhere classified Andrewartha, SJ Munns, SL Edwards, A Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish |
topic_facet |
Biological Sciences Other Biological Sciences Biological Sciences not elsewhere classified |
description |
Hemoglobin concentration [Hb] is measured for a wide variety of animal studies. The use of Point-of-care devices, such as the HemoCue, is becoming increasingly common due to their portability, relative ease of use and low cost. This study aimed to determine whether the [Hb] of blue-tongued skink ( Tiliqua nigrolutea ) blood can be accurately determined using the HemoCue and whether the HemoCue overestimates [Hb] of reptile blood in a similar manner to fish blood. We additionally aimed to test whether ploidy affected [Hb] determined by the HemoCue using blood from diploid and triploid Atlantic salmon ( Salmo salar ). The HemoCue Hb 201 + systematically overestimated [Hb] in both blue-tongued skinks and Atlantic salmon and there was no difference between calibration equations determined for diploid or triploid salmon. The overestimation was systematic in both species, and as such, [Hb] determined by the HemoCue can be corrected using appropriate calibration equations. |
format |
Article in Journal/Newspaper |
author |
Andrewartha, SJ Munns, SL Edwards, A |
author_facet |
Andrewartha, SJ Munns, SL Edwards, A |
author_sort |
Andrewartha, SJ |
title |
Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish |
title_short |
Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish |
title_full |
Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish |
title_fullStr |
Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish |
title_full_unstemmed |
Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish |
title_sort |
calibration of the hemocue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish |
publisher |
Oxford University Press |
publishDate |
2016 |
url |
https://doi.org/10.1093/conphys/cow006 http://ecite.utas.edu.au/106982 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://ecite.utas.edu.au/106982/1/Conserv Physiol-2016-Andrewartha.pdf http://dx.doi.org/10.1093/conphys/cow006 Andrewartha, SJ and Munns, SL and Edwards, A, Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish, Conservation Physiology, 4, (1) pp. 1-6. ISSN 2051-1434 (2016) [Refereed Article] http://ecite.utas.edu.au/106982 |
op_doi |
https://doi.org/10.1093/conphys/cow006 |
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Conservation Physiology |
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4 |
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1 |
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cow006 |
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1766361484156207104 |