Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L.
Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans whichaffects several cultured marine fish worldwide. The characterization of pro-inflammatory andimmune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at10 days post-inoculat...
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ftunivtasecite:oai:ecite.utas.edu.au:104995 2023-05-15T15:31:47+02:00 Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L. Pennacchi, Y Leef, MJ Crosbie, P Nowak, BF Bridle, AR 2015 application/pdf http://ecite.utas.edu.au/104995 en eng . http://ecite.utas.edu.au/104995/1/Proceedings isaah7-3b.pdf Pennacchi, Y and Leef, MJ and Crosbie, P and Nowak, BF and Bridle, AR, Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L. , Proceedings of the Seventh International Symposium on Aquatic Animal Health, 31 August - 4 September, Portland, Oregon, USA, pp. 59. (2015) [Conference Extract] http://ecite.utas.edu.au/104995 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Conference Extract NonPeerReviewed 2015 ftunivtasecite 2019-12-13T22:06:15Z Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans whichaffects several cultured marine fish worldwide. The characterization of pro-inflammatory andimmune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at10 days post-inoculation using 2D quantitative RT-PCR, a method of mapping transcriptionalresponses in tissues. This method is a variant of traditional quantitative RT-PCR whereby RNA wasextracted and reverse transcribed from gill samples obtained using a biopsy punch from designatedareas of the gill from fish. In theory this method could be used to map the transcriptional responsesthroughout the entire gill with the resolution of the mapped changes dependent on the number andsize of gill subsamples. In the present study we restricted our subsample area to eight 2 mm biopsypunches from the dorsal portion of the second left gill arch of each individual fish.The genes of interest were IL-1β, TNF-α, TCR-α chain, CD8, CD4, MHC-IIα, MHC-I, IgM andIgT. A significant increase in expression of the mRNA of all the genes was observed in the gills ofAGD-affected fish. Furthermore, a correlation analysis between CD8-α and TCR-α showed that themRNA expression of these two genes was strongly positively correlated (r = 0.9). Contrary toprevious studies, our data suggest that the parasite, N. perurans , elicits a classical inflammatoryresponse in the gills of AGD-affected fish and indicates that the mRNA expression of immunegenes within gill lesions misrepresents the cellular immune response in the gills during AGD. Conference Object Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) |
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eCite UTAS (University of Tasmania) |
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English |
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Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
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Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Pennacchi, Y Leef, MJ Crosbie, P Nowak, BF Bridle, AR Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L. |
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Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
description |
Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans whichaffects several cultured marine fish worldwide. The characterization of pro-inflammatory andimmune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at10 days post-inoculation using 2D quantitative RT-PCR, a method of mapping transcriptionalresponses in tissues. This method is a variant of traditional quantitative RT-PCR whereby RNA wasextracted and reverse transcribed from gill samples obtained using a biopsy punch from designatedareas of the gill from fish. In theory this method could be used to map the transcriptional responsesthroughout the entire gill with the resolution of the mapped changes dependent on the number andsize of gill subsamples. In the present study we restricted our subsample area to eight 2 mm biopsypunches from the dorsal portion of the second left gill arch of each individual fish.The genes of interest were IL-1β, TNF-α, TCR-α chain, CD8, CD4, MHC-IIα, MHC-I, IgM andIgT. A significant increase in expression of the mRNA of all the genes was observed in the gills ofAGD-affected fish. Furthermore, a correlation analysis between CD8-α and TCR-α showed that themRNA expression of these two genes was strongly positively correlated (r = 0.9). Contrary toprevious studies, our data suggest that the parasite, N. perurans , elicits a classical inflammatoryresponse in the gills of AGD-affected fish and indicates that the mRNA expression of immunegenes within gill lesions misrepresents the cellular immune response in the gills during AGD. |
format |
Conference Object |
author |
Pennacchi, Y Leef, MJ Crosbie, P Nowak, BF Bridle, AR |
author_facet |
Pennacchi, Y Leef, MJ Crosbie, P Nowak, BF Bridle, AR |
author_sort |
Pennacchi, Y |
title |
Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L. |
title_short |
Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L. |
title_full |
Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L. |
title_fullStr |
Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L. |
title_full_unstemmed |
Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L. |
title_sort |
evidence of immune and inflammatory processes in the gills of agd-affected atlantic salmon, salmo salar l. |
publisher |
. |
publishDate |
2015 |
url |
http://ecite.utas.edu.au/104995 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://ecite.utas.edu.au/104995/1/Proceedings isaah7-3b.pdf Pennacchi, Y and Leef, MJ and Crosbie, P and Nowak, BF and Bridle, AR, Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L. , Proceedings of the Seventh International Symposium on Aquatic Animal Health, 31 August - 4 September, Portland, Oregon, USA, pp. 59. (2015) [Conference Extract] http://ecite.utas.edu.au/104995 |
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