Alginate microencapsulation for oral immunisation of finfish: release characteristics, ex vivo intestinal uptake and in vivo administration in Atlantic salmon, Salmo salar L
This study examined the feasibility of alginate microcapsules manufactured using a low-impact technology and reagents to protect orally delivered immunogens for use as immunoprophylactics for fish. Physical characteristics and protein release kinetics of the microcapsules were examined at different...
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Online Access: | https://doi.org/10.1007/s10126-015-9663-7 http://www.ncbi.nlm.nih.gov/pubmed/26410294 http://ecite.utas.edu.au/103224 |
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ftunivtasecite:oai:ecite.utas.edu.au:103224 2023-05-15T15:30:57+02:00 Alginate microencapsulation for oral immunisation of finfish: release characteristics, ex vivo intestinal uptake and in vivo administration in Atlantic salmon, Salmo salar L Ghosh, B Nowak, BF Bridle, AR 2015 https://doi.org/10.1007/s10126-015-9663-7 http://www.ncbi.nlm.nih.gov/pubmed/26410294 http://ecite.utas.edu.au/103224 en eng Springer-Verlag http://dx.doi.org/10.1007/s10126-015-9663-7 Ghosh, B and Nowak, BF and Bridle, AR, Alginate microencapsulation for oral immunisation of finfish: release characteristics, ex vivo intestinal uptake and in vivo administration in Atlantic salmon, Salmo salar L, Marine Biotechnology, 17, (6) pp. 841-853. ISSN 1436-2228 (2015) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/26410294 http://ecite.utas.edu.au/103224 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Refereed Article PeerReviewed 2015 ftunivtasecite https://doi.org/10.1007/s10126-015-9663-7 2019-12-14T07:05:03Z This study examined the feasibility of alginate microcapsules manufactured using a low-impact technology and reagents to protect orally delivered immunogens for use as immunoprophylactics for fish. Physical characteristics and protein release kinetics of the microcapsules were examined at different pH and temperature levels using a microencapsulated model protein, bovine serum albumin (BSA). Impact of the microencapsulation process on contents was determined by analysing change in bioactivity of microencapsulated lysozyme. Feasibility of the method for oral immunoprophylaxis of finfish was assessed using FITC-labelled microcapsules. These were applied to distal intestinal explants of Atlantic salmon ( Salmo salar ) to investigate uptake ex vivo. Systemic distribution of microcapsules was investigated by oral administration of FITC-labelled microcapsules to Atlantic salmon fry by incorporating into feed. The microcapsules produced were structurally robust and retained surface integrity, with a modal size distribution of 250750nm and a tendency to aggregate. Entrapment efficiency of microencapsulation was 51.2% for BSA and 43.2% in the case of lysozyme. Microcapsules demonstrated controlled release of protein, which increased with increasing pH or temperature, and the process had no significant negative effect on bioactivity of lysozyme. Uptake of fluorescent-labelled microcapsules was clearly demonstrated by intestinal explants over a 24-h period. Evidence of microcapsules was found in the intestine, spleen, kidney and liver of fry following oral administration. Amenability of the microcapsules to intestinal uptake and distribution reinforced the strong potential for use of this microencapsulation method in oral immunoprophylaxis of finfish using sensitive immunogenic substances. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Marine Biotechnology 17 6 841 853 |
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Open Polar |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
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Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Ghosh, B Nowak, BF Bridle, AR Alginate microencapsulation for oral immunisation of finfish: release characteristics, ex vivo intestinal uptake and in vivo administration in Atlantic salmon, Salmo salar L |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
description |
This study examined the feasibility of alginate microcapsules manufactured using a low-impact technology and reagents to protect orally delivered immunogens for use as immunoprophylactics for fish. Physical characteristics and protein release kinetics of the microcapsules were examined at different pH and temperature levels using a microencapsulated model protein, bovine serum albumin (BSA). Impact of the microencapsulation process on contents was determined by analysing change in bioactivity of microencapsulated lysozyme. Feasibility of the method for oral immunoprophylaxis of finfish was assessed using FITC-labelled microcapsules. These were applied to distal intestinal explants of Atlantic salmon ( Salmo salar ) to investigate uptake ex vivo. Systemic distribution of microcapsules was investigated by oral administration of FITC-labelled microcapsules to Atlantic salmon fry by incorporating into feed. The microcapsules produced were structurally robust and retained surface integrity, with a modal size distribution of 250750nm and a tendency to aggregate. Entrapment efficiency of microencapsulation was 51.2% for BSA and 43.2% in the case of lysozyme. Microcapsules demonstrated controlled release of protein, which increased with increasing pH or temperature, and the process had no significant negative effect on bioactivity of lysozyme. Uptake of fluorescent-labelled microcapsules was clearly demonstrated by intestinal explants over a 24-h period. Evidence of microcapsules was found in the intestine, spleen, kidney and liver of fry following oral administration. Amenability of the microcapsules to intestinal uptake and distribution reinforced the strong potential for use of this microencapsulation method in oral immunoprophylaxis of finfish using sensitive immunogenic substances. |
format |
Article in Journal/Newspaper |
author |
Ghosh, B Nowak, BF Bridle, AR |
author_facet |
Ghosh, B Nowak, BF Bridle, AR |
author_sort |
Ghosh, B |
title |
Alginate microencapsulation for oral immunisation of finfish: release characteristics, ex vivo intestinal uptake and in vivo administration in Atlantic salmon, Salmo salar L |
title_short |
Alginate microencapsulation for oral immunisation of finfish: release characteristics, ex vivo intestinal uptake and in vivo administration in Atlantic salmon, Salmo salar L |
title_full |
Alginate microencapsulation for oral immunisation of finfish: release characteristics, ex vivo intestinal uptake and in vivo administration in Atlantic salmon, Salmo salar L |
title_fullStr |
Alginate microencapsulation for oral immunisation of finfish: release characteristics, ex vivo intestinal uptake and in vivo administration in Atlantic salmon, Salmo salar L |
title_full_unstemmed |
Alginate microencapsulation for oral immunisation of finfish: release characteristics, ex vivo intestinal uptake and in vivo administration in Atlantic salmon, Salmo salar L |
title_sort |
alginate microencapsulation for oral immunisation of finfish: release characteristics, ex vivo intestinal uptake and in vivo administration in atlantic salmon, salmo salar l |
publisher |
Springer-Verlag |
publishDate |
2015 |
url |
https://doi.org/10.1007/s10126-015-9663-7 http://www.ncbi.nlm.nih.gov/pubmed/26410294 http://ecite.utas.edu.au/103224 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://dx.doi.org/10.1007/s10126-015-9663-7 Ghosh, B and Nowak, BF and Bridle, AR, Alginate microencapsulation for oral immunisation of finfish: release characteristics, ex vivo intestinal uptake and in vivo administration in Atlantic salmon, Salmo salar L, Marine Biotechnology, 17, (6) pp. 841-853. ISSN 1436-2228 (2015) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/26410294 http://ecite.utas.edu.au/103224 |
op_doi |
https://doi.org/10.1007/s10126-015-9663-7 |
container_title |
Marine Biotechnology |
container_volume |
17 |
container_issue |
6 |
container_start_page |
841 |
op_container_end_page |
853 |
_version_ |
1766361433055952896 |