Proteomic insight into functional changes of proteorhodopsin-containing bacterial species Psychroflexus torquis under different illumination and salinity levels
The extremely psychrophilic proteorhodopsin-containing bacterial species Psychroflexus torquis is considered to be a model sea-ice microorganism, which has adapted to an epiphytic lifestyle. So far, not much is known about proteorhodopsin-based phototrophy and associated life strategies of sea ice b...
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Amer Chemical Soc
2015
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Online Access: | https://doi.org/10.1021/acs.jproteome.5b00241 http://www.ncbi.nlm.nih.gov/pubmed/26179671 http://ecite.utas.edu.au/102149 |
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ftunivtasecite:oai:ecite.utas.edu.au:102149 2023-05-15T18:17:55+02:00 Proteomic insight into functional changes of proteorhodopsin-containing bacterial species Psychroflexus torquis under different illumination and salinity levels Feng, S Powell, SM Wilson, R Bowman, JP 2015 https://doi.org/10.1021/acs.jproteome.5b00241 http://www.ncbi.nlm.nih.gov/pubmed/26179671 http://ecite.utas.edu.au/102149 en eng Amer Chemical Soc http://dx.doi.org/10.1021/acs.jproteome.5b00241 Feng, S and Powell, SM and Wilson, R and Bowman, JP, Proteomic insight into functional changes of proteorhodopsin-containing bacterial species Psychroflexus torquis under different illumination and salinity levels, Journal of Proteome Research, 14, (9) pp. 3848-3858. ISSN 1535-3893 (2015) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/26179671 http://ecite.utas.edu.au/102149 Biological Sciences Microbiology Microbial Ecology Refereed Article PeerReviewed 2015 ftunivtasecite https://doi.org/10.1021/acs.jproteome.5b00241 2019-12-13T22:03:45Z The extremely psychrophilic proteorhodopsin-containing bacterial species Psychroflexus torquis is considered to be a model sea-ice microorganism, which has adapted to an epiphytic lifestyle. So far, not much is known about proteorhodopsin-based phototrophy and associated life strategies of sea ice bacteria, although it has been previously shown that P. torquis can gain growth advantage from light using a proteorhodopsin proton pump, the activity of which is influenced by environmental salinity. The comprehensive quantitative proteomic study performed here indicated that P. torquis responds to changing salinity and illumination conditions. Proteins in the electron-transfer chain were down-regulated at a suboptimal salinity level, TonB-dependent transporters increased in abundance under supra-optimal salinity and decreased under suboptimal salinity. In addition, several anaplerotic CO 2 fixation proteins and three putative light sensing proteins that contain PAS and GAF domains became more abundant under illumination. Furthermore, central metabolic pathways (TCA and glycolysis) were also induced by both salinity stress and illumination. The data suggest that P. torquis responded to changes in both light energy and salinity to modulate membrane and central metabolic proteins that are involved in energy production as well as nutrient uptake and gliding motility processes that would be especially advantageous during the polar summer ice algal bloom. Article in Journal/Newspaper Sea ice eCite UTAS (University of Tasmania) Journal of Proteome Research 14 9 3848 3858 |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Biological Sciences Microbiology Microbial Ecology |
spellingShingle |
Biological Sciences Microbiology Microbial Ecology Feng, S Powell, SM Wilson, R Bowman, JP Proteomic insight into functional changes of proteorhodopsin-containing bacterial species Psychroflexus torquis under different illumination and salinity levels |
topic_facet |
Biological Sciences Microbiology Microbial Ecology |
description |
The extremely psychrophilic proteorhodopsin-containing bacterial species Psychroflexus torquis is considered to be a model sea-ice microorganism, which has adapted to an epiphytic lifestyle. So far, not much is known about proteorhodopsin-based phototrophy and associated life strategies of sea ice bacteria, although it has been previously shown that P. torquis can gain growth advantage from light using a proteorhodopsin proton pump, the activity of which is influenced by environmental salinity. The comprehensive quantitative proteomic study performed here indicated that P. torquis responds to changing salinity and illumination conditions. Proteins in the electron-transfer chain were down-regulated at a suboptimal salinity level, TonB-dependent transporters increased in abundance under supra-optimal salinity and decreased under suboptimal salinity. In addition, several anaplerotic CO 2 fixation proteins and three putative light sensing proteins that contain PAS and GAF domains became more abundant under illumination. Furthermore, central metabolic pathways (TCA and glycolysis) were also induced by both salinity stress and illumination. The data suggest that P. torquis responded to changes in both light energy and salinity to modulate membrane and central metabolic proteins that are involved in energy production as well as nutrient uptake and gliding motility processes that would be especially advantageous during the polar summer ice algal bloom. |
format |
Article in Journal/Newspaper |
author |
Feng, S Powell, SM Wilson, R Bowman, JP |
author_facet |
Feng, S Powell, SM Wilson, R Bowman, JP |
author_sort |
Feng, S |
title |
Proteomic insight into functional changes of proteorhodopsin-containing bacterial species Psychroflexus torquis under different illumination and salinity levels |
title_short |
Proteomic insight into functional changes of proteorhodopsin-containing bacterial species Psychroflexus torquis under different illumination and salinity levels |
title_full |
Proteomic insight into functional changes of proteorhodopsin-containing bacterial species Psychroflexus torquis under different illumination and salinity levels |
title_fullStr |
Proteomic insight into functional changes of proteorhodopsin-containing bacterial species Psychroflexus torquis under different illumination and salinity levels |
title_full_unstemmed |
Proteomic insight into functional changes of proteorhodopsin-containing bacterial species Psychroflexus torquis under different illumination and salinity levels |
title_sort |
proteomic insight into functional changes of proteorhodopsin-containing bacterial species psychroflexus torquis under different illumination and salinity levels |
publisher |
Amer Chemical Soc |
publishDate |
2015 |
url |
https://doi.org/10.1021/acs.jproteome.5b00241 http://www.ncbi.nlm.nih.gov/pubmed/26179671 http://ecite.utas.edu.au/102149 |
genre |
Sea ice |
genre_facet |
Sea ice |
op_relation |
http://dx.doi.org/10.1021/acs.jproteome.5b00241 Feng, S and Powell, SM and Wilson, R and Bowman, JP, Proteomic insight into functional changes of proteorhodopsin-containing bacterial species Psychroflexus torquis under different illumination and salinity levels, Journal of Proteome Research, 14, (9) pp. 3848-3858. ISSN 1535-3893 (2015) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/26179671 http://ecite.utas.edu.au/102149 |
op_doi |
https://doi.org/10.1021/acs.jproteome.5b00241 |
container_title |
Journal of Proteome Research |
container_volume |
14 |
container_issue |
9 |
container_start_page |
3848 |
op_container_end_page |
3858 |
_version_ |
1766193396835155968 |