Lower jaw deformity in triploid Atlantic salmon: an integrated morphological and transcriptomic investigation
Lower jaw deformity (LJD) is a skeletal abnormality that has been commonly reported in farmedAtlantic salmon in several countries at varying prevalence and predominantly in triploids (Benfey,2001; Sadler et al., 2001; Leclercq et al., 2011; Fraser et al., 2013; Taylor et al., 2013). In Tasmania,LJD...
Main Authors: | , , , , , , |
---|---|
Format: | Conference Object |
Language: | English |
Published: |
-
2015
|
Subjects: | |
Online Access: | http://ecite.utas.edu.au/101322 |
id |
ftunivtasecite:oai:ecite.utas.edu.au:101322 |
---|---|
record_format |
openpolar |
spelling |
ftunivtasecite:oai:ecite.utas.edu.au:101322 2023-05-15T15:31:07+02:00 Lower jaw deformity in triploid Atlantic salmon: an integrated morphological and transcriptomic investigation Amoroso, G Ventura, T Adams, M Carter, CG Battaglene, SC Elizur, A Cobcroft, JM 2015 application/pdf http://ecite.utas.edu.au/101322 en eng - http://ecite.utas.edu.au/101322/1/IAFSB15_Amoroso_presentation.pdf Amoroso, G and Ventura, T and Adams, M and Carter, CG and Battaglene, SC and Elizur, A and Cobcroft, JM, Lower jaw deformity in triploid Atlantic salmon: an integrated morphological and transcriptomic investigation, Interdisciplinary Approaches in Fish Skeletal Biology Abstracts, 27-30 April, Tavira, Algarve, Portugal (2015) [Conference Extract] http://ecite.utas.edu.au/101322 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Conference Extract NonPeerReviewed 2015 ftunivtasecite 2019-12-13T22:03:00Z Lower jaw deformity (LJD) is a skeletal abnormality that has been commonly reported in farmedAtlantic salmon in several countries at varying prevalence and predominantly in triploids (Benfey,2001; Sadler et al., 2001; Leclercq et al., 2011; Fraser et al., 2013; Taylor et al., 2013). In Tasmania,LJD can impact up to 30% of commercially produced all-female triploid Atlantic salmon leading toconsiderable loss to farmers (Hughes, 1992). Our study assessed the effect of ploidy (diploid and triploid) and temperature (14 and 18C) on theprevalence of several skeletal deformities in all-female Atlantic salmon from 3 to 60 g. Theprevalence of LJD was confirmed to be higher in triploids and no temperature effect was observed.Samples of LJD-affected and morphologically normal triploid fish were collected for molecularanalysis at the end of the developmental assessment (57.2 1.4 g) when the prevalence of LJD was10.9 3.5%. Recently, LJD occurrence has been linked to phosphorus deficiency in the diet of triploid salmon(Baeverfjord et al., 2009; Fjelldal et al., 2015). Little is known about the mechanisms underlying LJDin triploids, so we took a transcriptome-wide descriptive approach to examine changes in geneexpression in the jaw of normal and LJD-affected triploid fish. In addition, we performed RT-qPCRto test the effect of temperature (standard and high) in normal and LJD-affected triploid fish on theexpression of transcripts known to be related to bone and cartilage (i.e. alp, col1a1, mmp13,osteocalcin, trap and col2a1). Analysis of our transcriptomic database implicated numerous genes encoding fundamentalstructural proteins in LJD, suggesting it is a multigenic-regulated trait. Characterisation and analysisof known roles of the differentially expressed genes suggests that an impairment in cartilageformation might underlie the predisposition to develop LJD, which could be exacerbated by dietaryphosphorus deficiency. Conference Object Atlantic salmon eCite UTAS (University of Tasmania) Sadler ENVELOPE(-62.044,-62.044,-64.691,-64.691) |
institution |
Open Polar |
collection |
eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
spellingShingle |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Amoroso, G Ventura, T Adams, M Carter, CG Battaglene, SC Elizur, A Cobcroft, JM Lower jaw deformity in triploid Atlantic salmon: an integrated morphological and transcriptomic investigation |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
description |
Lower jaw deformity (LJD) is a skeletal abnormality that has been commonly reported in farmedAtlantic salmon in several countries at varying prevalence and predominantly in triploids (Benfey,2001; Sadler et al., 2001; Leclercq et al., 2011; Fraser et al., 2013; Taylor et al., 2013). In Tasmania,LJD can impact up to 30% of commercially produced all-female triploid Atlantic salmon leading toconsiderable loss to farmers (Hughes, 1992). Our study assessed the effect of ploidy (diploid and triploid) and temperature (14 and 18C) on theprevalence of several skeletal deformities in all-female Atlantic salmon from 3 to 60 g. Theprevalence of LJD was confirmed to be higher in triploids and no temperature effect was observed.Samples of LJD-affected and morphologically normal triploid fish were collected for molecularanalysis at the end of the developmental assessment (57.2 1.4 g) when the prevalence of LJD was10.9 3.5%. Recently, LJD occurrence has been linked to phosphorus deficiency in the diet of triploid salmon(Baeverfjord et al., 2009; Fjelldal et al., 2015). Little is known about the mechanisms underlying LJDin triploids, so we took a transcriptome-wide descriptive approach to examine changes in geneexpression in the jaw of normal and LJD-affected triploid fish. In addition, we performed RT-qPCRto test the effect of temperature (standard and high) in normal and LJD-affected triploid fish on theexpression of transcripts known to be related to bone and cartilage (i.e. alp, col1a1, mmp13,osteocalcin, trap and col2a1). Analysis of our transcriptomic database implicated numerous genes encoding fundamentalstructural proteins in LJD, suggesting it is a multigenic-regulated trait. Characterisation and analysisof known roles of the differentially expressed genes suggests that an impairment in cartilageformation might underlie the predisposition to develop LJD, which could be exacerbated by dietaryphosphorus deficiency. |
format |
Conference Object |
author |
Amoroso, G Ventura, T Adams, M Carter, CG Battaglene, SC Elizur, A Cobcroft, JM |
author_facet |
Amoroso, G Ventura, T Adams, M Carter, CG Battaglene, SC Elizur, A Cobcroft, JM |
author_sort |
Amoroso, G |
title |
Lower jaw deformity in triploid Atlantic salmon: an integrated morphological and transcriptomic investigation |
title_short |
Lower jaw deformity in triploid Atlantic salmon: an integrated morphological and transcriptomic investigation |
title_full |
Lower jaw deformity in triploid Atlantic salmon: an integrated morphological and transcriptomic investigation |
title_fullStr |
Lower jaw deformity in triploid Atlantic salmon: an integrated morphological and transcriptomic investigation |
title_full_unstemmed |
Lower jaw deformity in triploid Atlantic salmon: an integrated morphological and transcriptomic investigation |
title_sort |
lower jaw deformity in triploid atlantic salmon: an integrated morphological and transcriptomic investigation |
publisher |
- |
publishDate |
2015 |
url |
http://ecite.utas.edu.au/101322 |
long_lat |
ENVELOPE(-62.044,-62.044,-64.691,-64.691) |
geographic |
Sadler |
geographic_facet |
Sadler |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://ecite.utas.edu.au/101322/1/IAFSB15_Amoroso_presentation.pdf Amoroso, G and Ventura, T and Adams, M and Carter, CG and Battaglene, SC and Elizur, A and Cobcroft, JM, Lower jaw deformity in triploid Atlantic salmon: an integrated morphological and transcriptomic investigation, Interdisciplinary Approaches in Fish Skeletal Biology Abstracts, 27-30 April, Tavira, Algarve, Portugal (2015) [Conference Extract] http://ecite.utas.edu.au/101322 |
_version_ |
1766361600387710976 |