Vaccination with recombinant protein (r22C03), a putative attachment factor of Neoparamoeba perurans , against AGD in Atlantic salmon ( Salmo salar ) and implications of a co-infection with Yersinia ruckeri
Amoebic gill disease (AGD) affects salmonids during the marine grow-out phase in the Tasmanian industry and in other major salmonid producing countries. During the period post-transfer to seawater, the bacterial condition yersiniosis can also cause high levels of mortality in Atlantic salmon grown i...
Published in: | Fish & Shellfish Immunology |
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Language: | English |
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Academic Press Ltd Elsevier Science Ltd
2015
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Online Access: | https://doi.org/10.1016/j.fsi.2015.03.016 http://www.ncbi.nlm.nih.gov/pubmed/25804487 http://ecite.utas.edu.au/100831 |
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ftunivtasecite:oai:ecite.utas.edu.au:100831 2023-05-15T15:32:17+02:00 Vaccination with recombinant protein (r22C03), a putative attachment factor of Neoparamoeba perurans , against AGD in Atlantic salmon ( Salmo salar ) and implications of a co-infection with Yersinia ruckeri Valdenegro-Vega, VA Cook, M Crosbie, P Bridle, AR Nowak, BF 2015 https://doi.org/10.1016/j.fsi.2015.03.016 http://www.ncbi.nlm.nih.gov/pubmed/25804487 http://ecite.utas.edu.au/100831 en eng Academic Press Ltd Elsevier Science Ltd http://dx.doi.org/10.1016/j.fsi.2015.03.016 Valdenegro-Vega, VA and Cook, M and Crosbie, P and Bridle, AR and Nowak, BF, Vaccination with recombinant protein (r22C03), a putative attachment factor of Neoparamoeba perurans , against AGD in Atlantic salmon ( Salmo salar ) and implications of a co-infection with Yersinia ruckeri , Fish and Shellfish Immunology, 44, (2) pp. 592-602. ISSN 1050-4648 (2015) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/25804487 http://ecite.utas.edu.au/100831 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Refereed Article PeerReviewed 2015 ftunivtasecite https://doi.org/10.1016/j.fsi.2015.03.016 2019-12-13T22:02:34Z Amoebic gill disease (AGD) affects salmonids during the marine grow-out phase in the Tasmanian industry and in other major salmonid producing countries. During the period post-transfer to seawater, the bacterial condition yersiniosis can also cause high levels of mortality in Atlantic salmon grown in Tasmania, in addition to the hatchery outbreaks. The recombinant protein r 22C03, a mannose-binding protein-like (MBP-like) similar to attachment factors of other amoebae, was tested as a vaccine candidate against AGD in a large scale challenge trial. Fish were immunised with r 22C03 combined with FCA via intraperitoneal (i.p.) injection, and given a booster five weeks later by either i.p. injection (RP group) or by a dip-immersion (mRP). Fish were then challenged twice with Neoparamoeba perurans : the initial challenge 16 weeks after primary immunisation was terminated due to presence of ulcerative lesions in the skin of salmon; the second challenge was carried out after five weeks of treatment with oxytetracycline. These skin lesions might have been associated with a concurrent infection with Yersinia ruckeri , which was detected by real-time qPCR in serum of a large proportion of moribund and survivor fish after the AGD challenge. Before and during the N.perurans infection, levels of antibodies against r 22C03 were measured by ELISA in serum, skin mucus and supernatant from skin and gill explants. For the second challenge, the average size of AGD lesions was recorded from histology sections and survival curves were obtained. Before AGD challenge, r 22C03 induced antibody responses in serum and explants with both vaccination strategies. At the end of the challenge, levels of antibodies were lower than before challenge irrespective of treatment. Both vaccinated groups presented increased serum antibody responses, while only mRP presented antibody responses in skin mucus, and no significant antibody responses were measured in the explants. Antibodies did not confer protection to N.perurans infection, as no difference was observed in the survival curves of the vaccinated and control groups, and there was no effect on the gill lesion size. The concurrent yersiniosis infection probably represented more closely infection patterns observed in commercial settings. However, it could have interfered with the survival results and with the ability of the fish to respond to the amoebae infection. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Fish & Shellfish Immunology 44 2 592 602 |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
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Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Valdenegro-Vega, VA Cook, M Crosbie, P Bridle, AR Nowak, BF Vaccination with recombinant protein (r22C03), a putative attachment factor of Neoparamoeba perurans , against AGD in Atlantic salmon ( Salmo salar ) and implications of a co-infection with Yersinia ruckeri |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
description |
Amoebic gill disease (AGD) affects salmonids during the marine grow-out phase in the Tasmanian industry and in other major salmonid producing countries. During the period post-transfer to seawater, the bacterial condition yersiniosis can also cause high levels of mortality in Atlantic salmon grown in Tasmania, in addition to the hatchery outbreaks. The recombinant protein r 22C03, a mannose-binding protein-like (MBP-like) similar to attachment factors of other amoebae, was tested as a vaccine candidate against AGD in a large scale challenge trial. Fish were immunised with r 22C03 combined with FCA via intraperitoneal (i.p.) injection, and given a booster five weeks later by either i.p. injection (RP group) or by a dip-immersion (mRP). Fish were then challenged twice with Neoparamoeba perurans : the initial challenge 16 weeks after primary immunisation was terminated due to presence of ulcerative lesions in the skin of salmon; the second challenge was carried out after five weeks of treatment with oxytetracycline. These skin lesions might have been associated with a concurrent infection with Yersinia ruckeri , which was detected by real-time qPCR in serum of a large proportion of moribund and survivor fish after the AGD challenge. Before and during the N.perurans infection, levels of antibodies against r 22C03 were measured by ELISA in serum, skin mucus and supernatant from skin and gill explants. For the second challenge, the average size of AGD lesions was recorded from histology sections and survival curves were obtained. Before AGD challenge, r 22C03 induced antibody responses in serum and explants with both vaccination strategies. At the end of the challenge, levels of antibodies were lower than before challenge irrespective of treatment. Both vaccinated groups presented increased serum antibody responses, while only mRP presented antibody responses in skin mucus, and no significant antibody responses were measured in the explants. Antibodies did not confer protection to N.perurans infection, as no difference was observed in the survival curves of the vaccinated and control groups, and there was no effect on the gill lesion size. The concurrent yersiniosis infection probably represented more closely infection patterns observed in commercial settings. However, it could have interfered with the survival results and with the ability of the fish to respond to the amoebae infection. |
format |
Article in Journal/Newspaper |
author |
Valdenegro-Vega, VA Cook, M Crosbie, P Bridle, AR Nowak, BF |
author_facet |
Valdenegro-Vega, VA Cook, M Crosbie, P Bridle, AR Nowak, BF |
author_sort |
Valdenegro-Vega, VA |
title |
Vaccination with recombinant protein (r22C03), a putative attachment factor of Neoparamoeba perurans , against AGD in Atlantic salmon ( Salmo salar ) and implications of a co-infection with Yersinia ruckeri |
title_short |
Vaccination with recombinant protein (r22C03), a putative attachment factor of Neoparamoeba perurans , against AGD in Atlantic salmon ( Salmo salar ) and implications of a co-infection with Yersinia ruckeri |
title_full |
Vaccination with recombinant protein (r22C03), a putative attachment factor of Neoparamoeba perurans , against AGD in Atlantic salmon ( Salmo salar ) and implications of a co-infection with Yersinia ruckeri |
title_fullStr |
Vaccination with recombinant protein (r22C03), a putative attachment factor of Neoparamoeba perurans , against AGD in Atlantic salmon ( Salmo salar ) and implications of a co-infection with Yersinia ruckeri |
title_full_unstemmed |
Vaccination with recombinant protein (r22C03), a putative attachment factor of Neoparamoeba perurans , against AGD in Atlantic salmon ( Salmo salar ) and implications of a co-infection with Yersinia ruckeri |
title_sort |
vaccination with recombinant protein (r22c03), a putative attachment factor of neoparamoeba perurans , against agd in atlantic salmon ( salmo salar ) and implications of a co-infection with yersinia ruckeri |
publisher |
Academic Press Ltd Elsevier Science Ltd |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.fsi.2015.03.016 http://www.ncbi.nlm.nih.gov/pubmed/25804487 http://ecite.utas.edu.au/100831 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://dx.doi.org/10.1016/j.fsi.2015.03.016 Valdenegro-Vega, VA and Cook, M and Crosbie, P and Bridle, AR and Nowak, BF, Vaccination with recombinant protein (r22C03), a putative attachment factor of Neoparamoeba perurans , against AGD in Atlantic salmon ( Salmo salar ) and implications of a co-infection with Yersinia ruckeri , Fish and Shellfish Immunology, 44, (2) pp. 592-602. ISSN 1050-4648 (2015) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/25804487 http://ecite.utas.edu.au/100831 |
op_doi |
https://doi.org/10.1016/j.fsi.2015.03.016 |
container_title |
Fish & Shellfish Immunology |
container_volume |
44 |
container_issue |
2 |
container_start_page |
592 |
op_container_end_page |
602 |
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1766362799664005120 |