Effects of ocean acidification on the photosynthetic performance, carbonic anhydrase activity and growth of the giant kelp Macrocystis pyrifera

• Published in: Photosynthesis Research
• Main Authors: Fernandez, PA, Roleda, MY, Hurd, CL
• Format: Article in Journal/Newspaper
• Language: English
• Published: Kluwer Academic Publ 2015
• Subjects: Environmental Sciences; Ecological Applications; Ecological Impacts of Climate Change; Ocean acidification
• Online Access: https://doi.org/10.1007/s11120-015-0138-5; http://www.ncbi.nlm.nih.gov/pubmed/25869634; http://ecite.utas.edu.au/100172

Under ocean acidification (OA), the 200% increase in CO 2(aq) and the reduction of pH by 0.3-0.4 units are predicted to affect the carbon physiology and growth of macroalgae. Here we examined how the physiology of the giant kelp Macrocystis pyrifera is affected by elevated pCO 2 /low pH. Growth and photosynthetic rates, external and internal carbonic anhydrase (CA) activity, HCO 3 - versus CO 2 use were determined over a 7-day incubation at ambient pCO 2 400atm/pH 8.00 and a future OA treatment of pCO 2 1200 atm/pH 7.59. Neither the photosynthetic nor growth rates were changed by elevated CO 2 supply in the OA treatment. These results were explained by the greater use of HCO 3 - compared to CO 2 as an inorganic carbon (Ci) source to support photosynthesis. Macrocystis is a mixed HCO 3 - and CO 2 user that exhibits two effective mechanisms for HCO 3 - utilization; as predicted for species that possess carbon-concentrating mechanisms (CCMs), photosynthesis was not substantially affected by elevated pCO 2 . The internal CA activity was also unaffected by OA, and it remained high and active throughout the experiment; this suggests that HCO 3 - uptake via an anion exchange protein was not affected by OA. Our results suggest that photosynthetic Ci uptake and growth of Macrocystis will not be affected by elevated pCO 2 /low pH predicted for the future, but the combined effects with other environmental factors like temperature and nutrient availability could change the physiological response of Macrocystis to OA. Therefore, further studies will be important to elucidate how this species might respond to the global environmental change predicted for the ocean.