| Summary: | The Barn owl (Tyto alba) is an opportunistic species which feeds mainly on small mammals but also on birds, bats, reptiles, amphibians, insects and fishes. With regard to bats, several studies in Europe suggest that this group constitutes a small portion of the Barn owl diet representing less than 1% of its prey items. Through the analysis of 2,934 Barn owl pellets, collected between 2006 and 2014 in 27 sites/nests located in north Portugal, the remains of six bats belonging to five species were identified in a total of 9,103 prey items identified: the Western barbastelle (Barbastella barbastellus), the Grey long-eared bat (Plecotus austriacus), the Brown long-eared bat (Plecotus auritus), the European free-tailed bat (Tadarida teniotis) and the Common pipistrelle (Pipistrellus pipistrellus). These findings are of great interest as they represent new data on the Brown longeared bat and European free-tailed bat distributions, and allow to confirm an historical record of the Western barbastelle in the region
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