Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae
Reliable banding techniques are a major necessity for genetic research in oysters. In this study, we carried out the cytogenetic characterization of four oyster species (family Ostreidae) using restriction endonuclease treatments. Chromosomes were treated with three different restriction enzymes, st...
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ftunivtad:oai:repositorio.utad.pt:10348/1720 2024-05-19T07:39:18+00:00 Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae Leitão, Alexandra Chaves, Raquel Santos, Sara Guedes-Pinto, Henrique Boudry, Pierre 2012-03-14T14:15:44Z application/pdf http://hdl.handle.net/10348/1720 eng eng Springer Science Leitão, Alexandra; Chaves, Raquel; Santos, Sara; Guedes-Pinto, Henrique; Boudry, Pierre. Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: Comparative karyological analysis within Ostreidae, Trabalho apresentado em 15th International Chromosome Conference, In 15th International Chromosome Conference, West London, 2004. http://hdl.handle.net/10348/1720 open access article 2012 ftunivtad 2024-04-23T23:39:49Z Reliable banding techniques are a major necessity for genetic research in oysters. In this study, we carried out the cytogenetic characterization of four oyster species (family Ostreidae) using restriction endonuclease treatments. Chromosomes were treated with three different restriction enzymes, stained with Giemsa, and examined for banding patterns. The following species were studied: Crassostrea gigas (2n = 20; total number of bands with ApaI, 74; HaeIII, 61; PstI, 76), Crassostrea angulata (2n = 20; ApaI, 62; HaeIII, 61; PstI, 55) (subfamily Crassostreinae), Ostrea edulis (2n = 20; ApaI, 82; HaeIII, 59; PstI, 66), and Ostrea conchaphila (2n = 20; ApaI, 68; HaeIII, 62; PstI, 69) (subfamily Ostreinae). Treatment of samples with ApaI, HaeIII, and PstI produced specific banding patterns, which demonstrates the potential of these enzymes for chromosome banding in oysters. This is of special interest, since it has been recently shown in mammalian chromosomes that restriction enzyme banding is compatible with fluorescence in situ hybridization. This study therefore provides a fundamental step in genome mapping of oysters, since chromosome banding with restriction enzymes facilitates physical gene mapping in these important aquaculture species. The analysis of the banded karyotypes revealed a greater similarity within the genera of Crassostrea and Ostrea than between them Article in Journal/Newspaper Crassostrea gigas Universidade de Trás-os-Montes e Alto Douro (utad): Repositório |
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Open Polar |
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Universidade de Trás-os-Montes e Alto Douro (utad): Repositório |
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ftunivtad |
language |
English |
description |
Reliable banding techniques are a major necessity for genetic research in oysters. In this study, we carried out the cytogenetic characterization of four oyster species (family Ostreidae) using restriction endonuclease treatments. Chromosomes were treated with three different restriction enzymes, stained with Giemsa, and examined for banding patterns. The following species were studied: Crassostrea gigas (2n = 20; total number of bands with ApaI, 74; HaeIII, 61; PstI, 76), Crassostrea angulata (2n = 20; ApaI, 62; HaeIII, 61; PstI, 55) (subfamily Crassostreinae), Ostrea edulis (2n = 20; ApaI, 82; HaeIII, 59; PstI, 66), and Ostrea conchaphila (2n = 20; ApaI, 68; HaeIII, 62; PstI, 69) (subfamily Ostreinae). Treatment of samples with ApaI, HaeIII, and PstI produced specific banding patterns, which demonstrates the potential of these enzymes for chromosome banding in oysters. This is of special interest, since it has been recently shown in mammalian chromosomes that restriction enzyme banding is compatible with fluorescence in situ hybridization. This study therefore provides a fundamental step in genome mapping of oysters, since chromosome banding with restriction enzymes facilitates physical gene mapping in these important aquaculture species. The analysis of the banded karyotypes revealed a greater similarity within the genera of Crassostrea and Ostrea than between them |
format |
Article in Journal/Newspaper |
author |
Leitão, Alexandra Chaves, Raquel Santos, Sara Guedes-Pinto, Henrique Boudry, Pierre |
spellingShingle |
Leitão, Alexandra Chaves, Raquel Santos, Sara Guedes-Pinto, Henrique Boudry, Pierre Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae |
author_facet |
Leitão, Alexandra Chaves, Raquel Santos, Sara Guedes-Pinto, Henrique Boudry, Pierre |
author_sort |
Leitão, Alexandra |
title |
Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae |
title_short |
Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae |
title_full |
Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae |
title_fullStr |
Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae |
title_full_unstemmed |
Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae |
title_sort |
restriction enzyme digestion chromosome banding in crassostrea and ostrea species: comparative karyological analysis within ostreidae |
publisher |
Springer Science |
publishDate |
2012 |
url |
http://hdl.handle.net/10348/1720 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
Leitão, Alexandra; Chaves, Raquel; Santos, Sara; Guedes-Pinto, Henrique; Boudry, Pierre. Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: Comparative karyological analysis within Ostreidae, Trabalho apresentado em 15th International Chromosome Conference, In 15th International Chromosome Conference, West London, 2004. http://hdl.handle.net/10348/1720 |
op_rights |
open access |
_version_ |
1799478860804259840 |