Surfactant monolayer bending elasticity in lipase containing bicontinuous microemulsions
Lipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural subs...
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ftunivstutt:oai:elib.uni-stuttgart.de:11682/13619 2023-11-12T04:07:40+01:00 Surfactant monolayer bending elasticity in lipase containing bicontinuous microemulsions Engelskirchen, Sandra Wellert, Stefan Holderer, Olaf Frielinghaus, Henrich Laupheimer, Michaela Richter, Sven Nestl, Bettina Nebel, Bernd Hauer, Bernhard 2021 https://doi.org/10.18419/opus-13600 http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-136198 http://elib.uni-stuttgart.de/handle/11682/13619 en eng doi:10.3389/fchem.2020.613388 2296-2646 1866375415 http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-136198 http://elib.uni-stuttgart.de/handle/11682/13619 http://dx.doi.org/10.18419/opus-13600 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ 540 article 2021 ftunivstutt https://doi.org/10.18419/opus-1360010.3389/fchem.2020.613388 2023-10-22T22:48:54Z Lipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural substrates is the substantial insolubility of such substrates in water. The conversion of substrates, natural or non-natural, by lipases generally involves the presence of a water-oil interface. In the present paper, we exploit the fact that the presence of lipases, in particular the lipase from Candida antarctica B (CalB), changes the bending elastic properties of a surfactant monolayer in a bicontinuous microemulsion consisting of D2O/NaCl -n-(d)-octane-pentaethylene glycol monodecyl ether (C10E5) in a similar manner as previously observed for amphiphilic block-copolymers. To determine the bending elastic constant, we have used two approaches, small angle neutron scattering (SANS) and neutron spin echo (NSE) spectroscopy. The time-averaged structure from SANS showed a slight decrease in bending elasticity, while on nanosecond time scales as probed with NSE, a stiffening has been observed, which was attributed to adsorption/desorption mechanisms of CalB at the surfactant monolayer. The results allow to derive further information on the influence of CalB on the composition and bending elasticity of the surfactant monolayer itself as well as the underlying adsorption/desorption mechanism. Article in Journal/Newspaper Antarc* Antarctica OPUS - Publication Server of the University of Stuttgart |
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ftunivstutt |
language |
English |
topic |
540 |
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540 Engelskirchen, Sandra Wellert, Stefan Holderer, Olaf Frielinghaus, Henrich Laupheimer, Michaela Richter, Sven Nestl, Bettina Nebel, Bernd Hauer, Bernhard Surfactant monolayer bending elasticity in lipase containing bicontinuous microemulsions |
topic_facet |
540 |
description |
Lipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural substrates is the substantial insolubility of such substrates in water. The conversion of substrates, natural or non-natural, by lipases generally involves the presence of a water-oil interface. In the present paper, we exploit the fact that the presence of lipases, in particular the lipase from Candida antarctica B (CalB), changes the bending elastic properties of a surfactant monolayer in a bicontinuous microemulsion consisting of D2O/NaCl -n-(d)-octane-pentaethylene glycol monodecyl ether (C10E5) in a similar manner as previously observed for amphiphilic block-copolymers. To determine the bending elastic constant, we have used two approaches, small angle neutron scattering (SANS) and neutron spin echo (NSE) spectroscopy. The time-averaged structure from SANS showed a slight decrease in bending elasticity, while on nanosecond time scales as probed with NSE, a stiffening has been observed, which was attributed to adsorption/desorption mechanisms of CalB at the surfactant monolayer. The results allow to derive further information on the influence of CalB on the composition and bending elasticity of the surfactant monolayer itself as well as the underlying adsorption/desorption mechanism. |
format |
Article in Journal/Newspaper |
author |
Engelskirchen, Sandra Wellert, Stefan Holderer, Olaf Frielinghaus, Henrich Laupheimer, Michaela Richter, Sven Nestl, Bettina Nebel, Bernd Hauer, Bernhard |
author_facet |
Engelskirchen, Sandra Wellert, Stefan Holderer, Olaf Frielinghaus, Henrich Laupheimer, Michaela Richter, Sven Nestl, Bettina Nebel, Bernd Hauer, Bernhard |
author_sort |
Engelskirchen, Sandra |
title |
Surfactant monolayer bending elasticity in lipase containing bicontinuous microemulsions |
title_short |
Surfactant monolayer bending elasticity in lipase containing bicontinuous microemulsions |
title_full |
Surfactant monolayer bending elasticity in lipase containing bicontinuous microemulsions |
title_fullStr |
Surfactant monolayer bending elasticity in lipase containing bicontinuous microemulsions |
title_full_unstemmed |
Surfactant monolayer bending elasticity in lipase containing bicontinuous microemulsions |
title_sort |
surfactant monolayer bending elasticity in lipase containing bicontinuous microemulsions |
publishDate |
2021 |
url |
https://doi.org/10.18419/opus-13600 http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-136198 http://elib.uni-stuttgart.de/handle/11682/13619 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
doi:10.3389/fchem.2020.613388 2296-2646 1866375415 http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-136198 http://elib.uni-stuttgart.de/handle/11682/13619 http://dx.doi.org/10.18419/opus-13600 |
op_rights |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.18419/opus-1360010.3389/fchem.2020.613388 |
_version_ |
1782328253157998592 |