Myxosporean hyperparasites of gill monogeneans are basal to the Multivalvulida
Background: Myxosporeans are known from aquatic annelids but parasitism of platyhelminths by myxosporeans has not been widely reported. Hyperparasitism of gill monogeneans by Myxidium giardi has been reported from the European eel and Myxidium-like hyperparasites have also been observed during studi...
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BioMed Central
2011
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ftunivstirling:oai:dspace.stir.ac.uk:1893/9808 2023-05-15T16:08:41+02:00 Myxosporean hyperparasites of gill monogeneans are basal to the Multivalvulida Freeman, Mark Shinn, Andrew University of Malaya Institute of Aquaculture orcid:0000-0002-5434-2685 2011-11 application/pdf http://hdl.handle.net/1893/9808 https://doi.org/10.1186/1756-3305-4-220 http://dspace.stir.ac.uk/bitstream/1893/9808/1/Freeman%20Lim%20%20Shinn%20mono%20myxos.pdf en eng BioMed Central Freeman M & Shinn A (2011) Myxosporean hyperparasites of gill monogeneans are basal to the Multivalvulida. Parasites and Vectors, 4, Art. No.: 220. https://doi.org/10.1186/1756-3305-4-220 220 http://hdl.handle.net/1893/9808 doi:10.1186/1756-3305-4-220 22115202 WOS:000297936100001 2-s2.0-82055171891 751230 http://dspace.stir.ac.uk/bitstream/1893/9808/1/Freeman%20Lim%20%20Shinn%20mono%20myxos.pdf This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Parasites and Vectors, Volume 4, article 220, published by BioMed Central, available at: http://www.parasitesandvectors.com/content/4/1/220 http://creativecommons.org/licenses/by/4.0/ CC-BY Journal Article VoR - Version of Record 2011 ftunivstirling https://doi.org/10.1186/1756-3305-4-220 2022-06-13T18:44:10Z Background: Myxosporeans are known from aquatic annelids but parasitism of platyhelminths by myxosporeans has not been widely reported. Hyperparasitism of gill monogeneans by Myxidium giardi has been reported from the European eel and Myxidium-like hyperparasites have also been observed during studies of gill monogeneans from Malaysia and Japan. The present study aimed to collect new hyperparasite material from Malaysia for morphological and molecular descriptions. In addition, PCR screening of host fish was undertaken to determine whether they are also hosts for the myxosporean. Results: Heavy myxosporean infections were observed in monogeneans from two out of 14 fish and were detected from a further five fish using specific PCRs and pooled monogenean DNA. Positive DNA isolates were sequenced and were from a single species of myxosporean. Myxospore morphology was consistent with Myxidium with histozoic development in the parenchymal tissues of the monogenean. Simultaneous infections in the fish could not be confirmed microscopically; however, identical myxosporean DNA could be amplified from kidney, spleen and intestinal tract tissues using the specific PCR. Small subunit (SSU) rDNA for the myxosporean was amplified and was found to be most similar (92%) to that of another hyperparasitic myxosporean from a gill monogenean from Japan and to numerous multivalvulidan myxosporeans from the genus Kudoa (89-91%). Phylogenetic analyses placed the hyperparasite sequence basally to clades containing Kudoa, Unicapsula and Sphaerospora. Conclusions: The myxosporean infecting the gill monogenean, Diplectanocotyla gracilis, from the Indo-Pacific tarpon, Megalops cyprinoides, is described as a new species, Myxidium incomptavermi, based on a histozoic development in the monogenean host and its phylogenetic placement. We have demonstrated for the first time that a myxosporean hyperparasite of gill monogeneans is detectable in the fish host. However, myxospores could not be isolated from the fish and confirmation was by PCR ... Article in Journal/Newspaper European eel University of Stirling: Stirling Digital Research Repository Pacific Parasites & Vectors 4 1 |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
description |
Background: Myxosporeans are known from aquatic annelids but parasitism of platyhelminths by myxosporeans has not been widely reported. Hyperparasitism of gill monogeneans by Myxidium giardi has been reported from the European eel and Myxidium-like hyperparasites have also been observed during studies of gill monogeneans from Malaysia and Japan. The present study aimed to collect new hyperparasite material from Malaysia for morphological and molecular descriptions. In addition, PCR screening of host fish was undertaken to determine whether they are also hosts for the myxosporean. Results: Heavy myxosporean infections were observed in monogeneans from two out of 14 fish and were detected from a further five fish using specific PCRs and pooled monogenean DNA. Positive DNA isolates were sequenced and were from a single species of myxosporean. Myxospore morphology was consistent with Myxidium with histozoic development in the parenchymal tissues of the monogenean. Simultaneous infections in the fish could not be confirmed microscopically; however, identical myxosporean DNA could be amplified from kidney, spleen and intestinal tract tissues using the specific PCR. Small subunit (SSU) rDNA for the myxosporean was amplified and was found to be most similar (92%) to that of another hyperparasitic myxosporean from a gill monogenean from Japan and to numerous multivalvulidan myxosporeans from the genus Kudoa (89-91%). Phylogenetic analyses placed the hyperparasite sequence basally to clades containing Kudoa, Unicapsula and Sphaerospora. Conclusions: The myxosporean infecting the gill monogenean, Diplectanocotyla gracilis, from the Indo-Pacific tarpon, Megalops cyprinoides, is described as a new species, Myxidium incomptavermi, based on a histozoic development in the monogenean host and its phylogenetic placement. We have demonstrated for the first time that a myxosporean hyperparasite of gill monogeneans is detectable in the fish host. However, myxospores could not be isolated from the fish and confirmation was by PCR ... |
author2 |
University of Malaya Institute of Aquaculture orcid:0000-0002-5434-2685 |
format |
Article in Journal/Newspaper |
author |
Freeman, Mark Shinn, Andrew |
spellingShingle |
Freeman, Mark Shinn, Andrew Myxosporean hyperparasites of gill monogeneans are basal to the Multivalvulida |
author_facet |
Freeman, Mark Shinn, Andrew |
author_sort |
Freeman, Mark |
title |
Myxosporean hyperparasites of gill monogeneans are basal to the Multivalvulida |
title_short |
Myxosporean hyperparasites of gill monogeneans are basal to the Multivalvulida |
title_full |
Myxosporean hyperparasites of gill monogeneans are basal to the Multivalvulida |
title_fullStr |
Myxosporean hyperparasites of gill monogeneans are basal to the Multivalvulida |
title_full_unstemmed |
Myxosporean hyperparasites of gill monogeneans are basal to the Multivalvulida |
title_sort |
myxosporean hyperparasites of gill monogeneans are basal to the multivalvulida |
publisher |
BioMed Central |
publishDate |
2011 |
url |
http://hdl.handle.net/1893/9808 https://doi.org/10.1186/1756-3305-4-220 http://dspace.stir.ac.uk/bitstream/1893/9808/1/Freeman%20Lim%20%20Shinn%20mono%20myxos.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
European eel |
genre_facet |
European eel |
op_relation |
Freeman M & Shinn A (2011) Myxosporean hyperparasites of gill monogeneans are basal to the Multivalvulida. Parasites and Vectors, 4, Art. No.: 220. https://doi.org/10.1186/1756-3305-4-220 220 http://hdl.handle.net/1893/9808 doi:10.1186/1756-3305-4-220 22115202 WOS:000297936100001 2-s2.0-82055171891 751230 http://dspace.stir.ac.uk/bitstream/1893/9808/1/Freeman%20Lim%20%20Shinn%20mono%20myxos.pdf |
op_rights |
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Parasites and Vectors, Volume 4, article 220, published by BioMed Central, available at: http://www.parasitesandvectors.com/content/4/1/220 http://creativecommons.org/licenses/by/4.0/ |
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CC-BY |
op_doi |
https://doi.org/10.1186/1756-3305-4-220 |
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