Photoperiod effects on the expression of kisspeptin and gonadotropin genes in Atlantic cod, Gadus morhua, during first maturation
In order to investigate the potential role of the kisspeptin system in the entrainment of reproduction in Atlantic cod, qPCR assays were developed for kiss2 and its receptor kissr4. mRNA expression was monitored in the brain over a full reproductive cycle in 2 populations of males and females: 1) a...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/9350 2023-05-15T15:26:59+02:00 Photoperiod effects on the expression of kisspeptin and gonadotropin genes in Atlantic cod, Gadus morhua, during first maturation Cowan, Mairi Davie, Andrew Migaud, Herve Machrihanish Institute of Aquaculture orcid:0000-0002-9524-618X orcid:0000-0002-5404-7512 2012-09 application/pdf http://hdl.handle.net/1893/9350 https://doi.org/10.1016/j.cbpa.2012.05.191 http://dspace.stir.ac.uk/bitstream/1893/9350/1/migaud_CBPA_2012.pdf en eng Elsevier Cowan M, Davie A & Migaud H (2012) Photoperiod effects on the expression of kisspeptin and gonadotropin genes in Atlantic cod, Gadus morhua, during first maturation. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 163 (1), pp. 82-94. https://doi.org/10.1016/j.cbpa.2012.05.191 http://hdl.handle.net/1893/9350 doi:10.1016/j.cbpa.2012.05.191 22613785 WOS:000306634400011 2-s2.0-84862878996 889649 http://dspace.stir.ac.uk/bitstream/1893/9350/1/migaud_CBPA_2012.pdf The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. http://www.rioxx.net/licenses/under-embargo-all-rights-reserved 2999-12-31 [migaud_CBPA_2012.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work. Atlantic cod Kisspeptin Brain Maturation Continuous light Journal Article VoR - Version of Record 2012 ftunivstirling https://doi.org/10.1016/j.cbpa.2012.05.191 2022-06-13T18:44:32Z In order to investigate the potential role of the kisspeptin system in the entrainment of reproduction in Atlantic cod, qPCR assays were developed for kiss2 and its receptor kissr4. mRNA expression was monitored in the brain over a full reproductive cycle in 2 populations of males and females: 1) a maturing population (exposed to simulated natural photoperiod, SNP) and 2) a maturation inhibited population (exposed to constant light, LL). Pituitary expression of gonadotropin subunit mRNA (fshβ and lhβ) was also measured. Results from this study indicated no clear temporal pattern in expression of kiss2 or kissr4 mRNAs in either population of cod, however acute elevations were apparent in maturing (SNP) individuals, namely an elevation in kiss2 in vitellogenic females and spermiating males and spikes in kissr4 during early vitellogenesis in females and spermatogenesis in males. Gonadotropin mRNA expression displayed strong amplitudinal changes over time with fshβ and lhβ mRNA expression increasing towards spawning in maturing individuals. No firm conclusions on the role of the kisspeptin system in cod puberty can be drawn at this stage, however mRNA increases in kiss2 and kissr4 may elude to conserved kisspeptin functions in cod and opens up interesting avenues on potential gender specific functions. Article in Journal/Newspaper atlantic cod Gadus morhua University of Stirling: Stirling Digital Research Repository Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 163 1 82 94 |
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Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Atlantic cod Kisspeptin Brain Maturation Continuous light |
spellingShingle |
Atlantic cod Kisspeptin Brain Maturation Continuous light Cowan, Mairi Davie, Andrew Migaud, Herve Photoperiod effects on the expression of kisspeptin and gonadotropin genes in Atlantic cod, Gadus morhua, during first maturation |
topic_facet |
Atlantic cod Kisspeptin Brain Maturation Continuous light |
description |
In order to investigate the potential role of the kisspeptin system in the entrainment of reproduction in Atlantic cod, qPCR assays were developed for kiss2 and its receptor kissr4. mRNA expression was monitored in the brain over a full reproductive cycle in 2 populations of males and females: 1) a maturing population (exposed to simulated natural photoperiod, SNP) and 2) a maturation inhibited population (exposed to constant light, LL). Pituitary expression of gonadotropin subunit mRNA (fshβ and lhβ) was also measured. Results from this study indicated no clear temporal pattern in expression of kiss2 or kissr4 mRNAs in either population of cod, however acute elevations were apparent in maturing (SNP) individuals, namely an elevation in kiss2 in vitellogenic females and spermiating males and spikes in kissr4 during early vitellogenesis in females and spermatogenesis in males. Gonadotropin mRNA expression displayed strong amplitudinal changes over time with fshβ and lhβ mRNA expression increasing towards spawning in maturing individuals. No firm conclusions on the role of the kisspeptin system in cod puberty can be drawn at this stage, however mRNA increases in kiss2 and kissr4 may elude to conserved kisspeptin functions in cod and opens up interesting avenues on potential gender specific functions. |
author2 |
Machrihanish Institute of Aquaculture orcid:0000-0002-9524-618X orcid:0000-0002-5404-7512 |
format |
Article in Journal/Newspaper |
author |
Cowan, Mairi Davie, Andrew Migaud, Herve |
author_facet |
Cowan, Mairi Davie, Andrew Migaud, Herve |
author_sort |
Cowan, Mairi |
title |
Photoperiod effects on the expression of kisspeptin and gonadotropin genes in Atlantic cod, Gadus morhua, during first maturation |
title_short |
Photoperiod effects on the expression of kisspeptin and gonadotropin genes in Atlantic cod, Gadus morhua, during first maturation |
title_full |
Photoperiod effects on the expression of kisspeptin and gonadotropin genes in Atlantic cod, Gadus morhua, during first maturation |
title_fullStr |
Photoperiod effects on the expression of kisspeptin and gonadotropin genes in Atlantic cod, Gadus morhua, during first maturation |
title_full_unstemmed |
Photoperiod effects on the expression of kisspeptin and gonadotropin genes in Atlantic cod, Gadus morhua, during first maturation |
title_sort |
photoperiod effects on the expression of kisspeptin and gonadotropin genes in atlantic cod, gadus morhua, during first maturation |
publisher |
Elsevier |
publishDate |
2012 |
url |
http://hdl.handle.net/1893/9350 https://doi.org/10.1016/j.cbpa.2012.05.191 http://dspace.stir.ac.uk/bitstream/1893/9350/1/migaud_CBPA_2012.pdf |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
Cowan M, Davie A & Migaud H (2012) Photoperiod effects on the expression of kisspeptin and gonadotropin genes in Atlantic cod, Gadus morhua, during first maturation. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 163 (1), pp. 82-94. https://doi.org/10.1016/j.cbpa.2012.05.191 http://hdl.handle.net/1893/9350 doi:10.1016/j.cbpa.2012.05.191 22613785 WOS:000306634400011 2-s2.0-84862878996 889649 http://dspace.stir.ac.uk/bitstream/1893/9350/1/migaud_CBPA_2012.pdf |
op_rights |
The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. http://www.rioxx.net/licenses/under-embargo-all-rights-reserved 2999-12-31 [migaud_CBPA_2012.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work. |
op_doi |
https://doi.org/10.1016/j.cbpa.2012.05.191 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
163 |
container_issue |
1 |
container_start_page |
82 |
op_container_end_page |
94 |
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