Fatty acyl desaturation in isolated hepatocytes from Atlantic salmon (Salmo salar): Stimulation by dietary borage oil containing gamma-linolenic acid
The effects of different dietary oils on the fatty acid compositions of liver phospholipids and the desaturation and elongation of [1-14C]18∶3n−3 and [1-14C]18∶2n−6 were investigated in isolated hepatocytes from Atlantic salmon. Atlantic salmon smolts were fed diets containing either a standard fish...
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ftunivstirling:oai:dspace.stir.ac.uk:1893/7561 2023-05-15T15:31:29+02:00 Fatty acyl desaturation in isolated hepatocytes from Atlantic salmon (Salmo salar): Stimulation by dietary borage oil containing gamma-linolenic acid Tocher, Douglas R Bell, J Gordon Dick, James R Sargent, John R Institute of Aquaculture University of Stirling orcid:0000-0002-8603-9410 1997-12 application/pdf http://hdl.handle.net/1893/7561 https://doi.org/10.1007/s11745-006-0159-0 http://dspace.stir.ac.uk/bitstream/1893/7561/1/tocher_lipids32.12_1997.pdf en eng Springer Tocher DR, Bell JG, Dick JR & Sargent JR (1997) Fatty acyl desaturation in isolated hepatocytes from Atlantic salmon (Salmo salar): Stimulation by dietary borage oil containing gamma-linolenic acid. Lipids, 32 (12), pp. 1237-1247. https://doi.org/10.1007/s11745-006-0159-0 http://hdl.handle.net/1893/7561 doi:10.1007/s11745-006-0159-0 9438233 WOS:000071180400001 2-s2.0-0031459692 890263 http://dspace.stir.ac.uk/bitstream/1893/7561/1/tocher_lipids32.12_1997.pdf The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. http://www.rioxx.net/licenses/under-embargo-all-rights-reserved 3000-01-01 [tocher_lipids32.12_1997.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work. Journal Article VoR - Version of Record 1997 ftunivstirling https://doi.org/10.1007/s11745-006-0159-0 2022-06-13T18:42:36Z The effects of different dietary oils on the fatty acid compositions of liver phospholipids and the desaturation and elongation of [1-14C]18∶3n−3 and [1-14C]18∶2n−6 were investigated in isolated hepatocytes from Atlantic salmon. Atlantic salmon smolts were fed diets containing either a standard fish oil (FO) as a control diet, a 1∶1 blend of Southern Hemisphere marine oil and tuna orbital oil (MO/TO), sunflower oil (SO), borage oil (BO), or oliver oil (OO) for 12 wk. The SO and BO diets significantly increased the percentages of 18:2n−6, 18:3n−6, 20:2n−6, 20:3n−6, and total n-6 polyunsaturated fatty acids (PUFA) in salmon liver lipids in comparison with the FO diet. The BO diet also increased the percentage of 20:4n−6. Both the SO and BO diets significantly reduced the percentages of all n−3 PUFA in comparison with the FO diet. The OO diet significantly increased the percentages of 18:1n−9, 18:2n−6, total monoenes, and total n−6 PUFA in liver lipids compared to the FO diet, and the percentages of all n−3 PUFA were significantly reduced. With [1-14C]18:3n−3, the recovery of radioactivity in the products of Δ6 desaturation was significantly greater in the hepatocytes from salmon fed SO, BO, and OO in comparison with the FO diet. The BO diet also increased the recovery of radioactivity in the products of Δ5 desaturation. Only the BO diet significantly affected the desaturation of [1-14C]18:2n−6, increasing recovery of radioactivity in both Δ6- and Δ5-desaturation products. In conclusion, dietary BO, enriched in γ-linolenic acid (18:3n−6), significantly increased the proportions of both 20:3n−6 and 20:4n−6 in salmon liver phospholipids and also significantly increased the desaturation of both 18:2n−6 and 18:3n−3 in salmon hepatocytes. The possible relationships between dietary fatty acid composition, tissue phospholipid fatty acid composition, and desaturation/elongation activities are discussed. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Lipids 32 12 1237 1247 |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
description |
The effects of different dietary oils on the fatty acid compositions of liver phospholipids and the desaturation and elongation of [1-14C]18∶3n−3 and [1-14C]18∶2n−6 were investigated in isolated hepatocytes from Atlantic salmon. Atlantic salmon smolts were fed diets containing either a standard fish oil (FO) as a control diet, a 1∶1 blend of Southern Hemisphere marine oil and tuna orbital oil (MO/TO), sunflower oil (SO), borage oil (BO), or oliver oil (OO) for 12 wk. The SO and BO diets significantly increased the percentages of 18:2n−6, 18:3n−6, 20:2n−6, 20:3n−6, and total n-6 polyunsaturated fatty acids (PUFA) in salmon liver lipids in comparison with the FO diet. The BO diet also increased the percentage of 20:4n−6. Both the SO and BO diets significantly reduced the percentages of all n−3 PUFA in comparison with the FO diet. The OO diet significantly increased the percentages of 18:1n−9, 18:2n−6, total monoenes, and total n−6 PUFA in liver lipids compared to the FO diet, and the percentages of all n−3 PUFA were significantly reduced. With [1-14C]18:3n−3, the recovery of radioactivity in the products of Δ6 desaturation was significantly greater in the hepatocytes from salmon fed SO, BO, and OO in comparison with the FO diet. The BO diet also increased the recovery of radioactivity in the products of Δ5 desaturation. Only the BO diet significantly affected the desaturation of [1-14C]18:2n−6, increasing recovery of radioactivity in both Δ6- and Δ5-desaturation products. In conclusion, dietary BO, enriched in γ-linolenic acid (18:3n−6), significantly increased the proportions of both 20:3n−6 and 20:4n−6 in salmon liver phospholipids and also significantly increased the desaturation of both 18:2n−6 and 18:3n−3 in salmon hepatocytes. The possible relationships between dietary fatty acid composition, tissue phospholipid fatty acid composition, and desaturation/elongation activities are discussed. |
author2 |
Institute of Aquaculture University of Stirling orcid:0000-0002-8603-9410 |
format |
Article in Journal/Newspaper |
author |
Tocher, Douglas R Bell, J Gordon Dick, James R Sargent, John R |
spellingShingle |
Tocher, Douglas R Bell, J Gordon Dick, James R Sargent, John R Fatty acyl desaturation in isolated hepatocytes from Atlantic salmon (Salmo salar): Stimulation by dietary borage oil containing gamma-linolenic acid |
author_facet |
Tocher, Douglas R Bell, J Gordon Dick, James R Sargent, John R |
author_sort |
Tocher, Douglas R |
title |
Fatty acyl desaturation in isolated hepatocytes from Atlantic salmon (Salmo salar): Stimulation by dietary borage oil containing gamma-linolenic acid |
title_short |
Fatty acyl desaturation in isolated hepatocytes from Atlantic salmon (Salmo salar): Stimulation by dietary borage oil containing gamma-linolenic acid |
title_full |
Fatty acyl desaturation in isolated hepatocytes from Atlantic salmon (Salmo salar): Stimulation by dietary borage oil containing gamma-linolenic acid |
title_fullStr |
Fatty acyl desaturation in isolated hepatocytes from Atlantic salmon (Salmo salar): Stimulation by dietary borage oil containing gamma-linolenic acid |
title_full_unstemmed |
Fatty acyl desaturation in isolated hepatocytes from Atlantic salmon (Salmo salar): Stimulation by dietary borage oil containing gamma-linolenic acid |
title_sort |
fatty acyl desaturation in isolated hepatocytes from atlantic salmon (salmo salar): stimulation by dietary borage oil containing gamma-linolenic acid |
publisher |
Springer |
publishDate |
1997 |
url |
http://hdl.handle.net/1893/7561 https://doi.org/10.1007/s11745-006-0159-0 http://dspace.stir.ac.uk/bitstream/1893/7561/1/tocher_lipids32.12_1997.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Tocher DR, Bell JG, Dick JR & Sargent JR (1997) Fatty acyl desaturation in isolated hepatocytes from Atlantic salmon (Salmo salar): Stimulation by dietary borage oil containing gamma-linolenic acid. Lipids, 32 (12), pp. 1237-1247. https://doi.org/10.1007/s11745-006-0159-0 http://hdl.handle.net/1893/7561 doi:10.1007/s11745-006-0159-0 9438233 WOS:000071180400001 2-s2.0-0031459692 890263 http://dspace.stir.ac.uk/bitstream/1893/7561/1/tocher_lipids32.12_1997.pdf |
op_rights |
The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. http://www.rioxx.net/licenses/under-embargo-all-rights-reserved 3000-01-01 [tocher_lipids32.12_1997.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work. |
op_doi |
https://doi.org/10.1007/s11745-006-0159-0 |
container_title |
Lipids |
container_volume |
32 |
container_issue |
12 |
container_start_page |
1237 |
op_container_end_page |
1247 |
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1766362011804893184 |