A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus)

Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for...

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Published in:Journal of Bacteriology
Main Authors: Sun, Boguang, Zhang, Xiao-Hua, Tang, Xuexi, Wang, Shushan, Zhong, Yingbin, Chen, Jixiang, Austin, Brian
Other Authors: Ocean University of China, Institute of Aquaculture
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 2007
Subjects:
Scophthalmus maximus
Turbot
Online Access:http://hdl.handle.net/1893/7387
https://doi.org/10.1128/JB.01650-06
http://dspace.stir.ac.uk/bitstream/1893/7387/1/Austin_2007_A_Single_Residue_Change.pdf
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spelling ftunivstirling:oai:dspace.stir.ac.uk:1893/7387 2023-05-15T18:15:41+02:00 A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus) Sun, Boguang Zhang, Xiao-Hua Tang, Xuexi Wang, Shushan Zhong, Yingbin Chen, Jixiang Austin, Brian Ocean University of China Institute of Aquaculture 2007-03 application/pdf http://hdl.handle.net/1893/7387 https://doi.org/10.1128/JB.01650-06 http://dspace.stir.ac.uk/bitstream/1893/7387/1/Austin_2007_A_Single_Residue_Change.pdf en eng American Society for Microbiology Sun B, Zhang X, Tang X, Wang S, Zhong Y, Chen J & Austin B (2007) A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus). Journal of Bacteriology, 189 (6), pp. 2575-2579. https://doi.org/10.1128/JB.01650-06 http://hdl.handle.net/1893/7387 doi:10.1128/JB.01650-06 WOS:000244985600042 785581 http://dspace.stir.ac.uk/bitstream/1893/7387/1/Austin_2007_A_Single_Residue_Change.pdf Publisher allows this work to be made available in this repository. Published in Journal of Bacteriology by American Society for Microbiology with the following policy: Authors may post their articles in full on personal or employer websites. ASM grants the author the right to post his/her article (after publication by ASM) on the author’s personal or university-hosted website, but not on any corporate, government, or similar website, without ASM’s prior permission, provided that proper credit is given to the original ASM publication. Journal Article VoR - Version of Record 2007 ftunivstirling https://doi.org/10.1128/JB.01650-06 2022-06-13T18:41:46Z Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish. Article in Journal/Newspaper Scophthalmus maximus Turbot University of Stirling: Stirling Digital Research Repository Journal of Bacteriology 189 6 2575 2579
institution Open Polar
collection University of Stirling: Stirling Digital Research Repository
op_collection_id ftunivstirling
language English
description Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish.
author2 Ocean University of China
Institute of Aquaculture
format Article in Journal/Newspaper
author Sun, Boguang
Zhang, Xiao-Hua
Tang, Xuexi
Wang, Shushan
Zhong, Yingbin
Chen, Jixiang
Austin, Brian
spellingShingle Sun, Boguang
Zhang, Xiao-Hua
Tang, Xuexi
Wang, Shushan
Zhong, Yingbin
Chen, Jixiang
Austin, Brian
A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus)
author_facet Sun, Boguang
Zhang, Xiao-Hua
Tang, Xuexi
Wang, Shushan
Zhong, Yingbin
Chen, Jixiang
Austin, Brian
author_sort Sun, Boguang
title A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus)
title_short A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus)
title_full A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus)
title_fullStr A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus)
title_full_unstemmed A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus)
title_sort single residue change in vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (scophthalmus maximus)
publisher American Society for Microbiology
publishDate 2007
url http://hdl.handle.net/1893/7387
https://doi.org/10.1128/JB.01650-06
http://dspace.stir.ac.uk/bitstream/1893/7387/1/Austin_2007_A_Single_Residue_Change.pdf
genre Scophthalmus maximus
Turbot
genre_facet Scophthalmus maximus
Turbot
op_relation Sun B, Zhang X, Tang X, Wang S, Zhong Y, Chen J & Austin B (2007) A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus). Journal of Bacteriology, 189 (6), pp. 2575-2579. https://doi.org/10.1128/JB.01650-06
http://hdl.handle.net/1893/7387
doi:10.1128/JB.01650-06
WOS:000244985600042
785581
http://dspace.stir.ac.uk/bitstream/1893/7387/1/Austin_2007_A_Single_Residue_Change.pdf
op_rights Publisher allows this work to be made available in this repository. Published in Journal of Bacteriology by American Society for Microbiology with the following policy: Authors may post their articles in full on personal or employer websites. ASM grants the author the right to post his/her article (after publication by ASM) on the author’s personal or university-hosted website, but not on any corporate, government, or similar website, without ASM’s prior permission, provided that proper credit is given to the original ASM publication.
op_doi https://doi.org/10.1128/JB.01650-06
container_title Journal of Bacteriology
container_volume 189
container_issue 6
container_start_page 2575
op_container_end_page 2579
_version_ 1766188866956427264

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