A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus)

Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for...

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Bibliographic Details
Published in:Journal of Bacteriology
Main Authors: Sun, Boguang, Zhang, Xiao-Hua, Tang, Xuexi, Wang, Shushan, Zhong, Yingbin, Chen, Jixiang, Austin, Brian
Other Authors: Ocean University of China, Institute of Aquaculture
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 2007
Subjects:
Scophthalmus maximus
Turbot
Online Access:http://hdl.handle.net/1893/7387
https://doi.org/10.1128/JB.01650-06
http://dspace.stir.ac.uk/bitstream/1893/7387/1/Austin_2007_A_Single_Residue_Change.pdf
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Summary:Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish.

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