Identification of immune-related genes from kidney and spleen of turbot, Psetta maxima (L.), by suppression subtractive hybridization following challenge with Vibrio harveyi
Suppression subtractive hybridization was used to investigate the response of turbot, Psetta maxima (L.), to Vibrio harveyi, by using a cDNA library constructed from artificially infected turbot kidney and spleen mRNA. Forty-nine expressed sequence tags were obtained. Several immune system genes wer...
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Online Access: | http://hdl.handle.net/1893/7215 https://doi.org/10.1111/j.1365-2761.2008.00914.x http://dspace.stir.ac.uk/bitstream/1893/7215/1/austin_immune-relatedgenes_2008.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/7215 2023-05-15T15:27:41+02:00 Identification of immune-related genes from kidney and spleen of turbot, Psetta maxima (L.), by suppression subtractive hybridization following challenge with Vibrio harveyi Wang, Chunzai Zhang, Xiao-Hua Jia, A Chen, Jixiang Austin, Brian Ocean University of China Heriot-Watt University 2008-07 application/pdf http://hdl.handle.net/1893/7215 https://doi.org/10.1111/j.1365-2761.2008.00914.x http://dspace.stir.ac.uk/bitstream/1893/7215/1/austin_immune-relatedgenes_2008.pdf en eng Wiley-Blackwell Wang C, Zhang X, Jia A, Chen J & Austin B (2008) Identification of immune-related genes from kidney and spleen of turbot, Psetta maxima (L.), by suppression subtractive hybridization following challenge with Vibrio harveyi. Journal of Fish Diseases, 31 (7), pp. 505-514. https://doi.org/10.1111/j.1365-2761.2008.00914.x http://hdl.handle.net/1893/7215 doi:10.1111/j.1365-2761.2008.00914.x WOS:000256636000004 785712 http://dspace.stir.ac.uk/bitstream/1893/7215/1/austin_immune-relatedgenes_2008.pdf The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. http://www.rioxx.net/licenses/under-embargo-all-rights-reserved 3000-01-01 [austin_immune-relatedgenes_2008.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work. immune-related gene MHC class Ia molecular identification quantitative PCR suppression subtractive hybridization Journal Article VoR - Version of Record 2008 ftunivstirling https://doi.org/10.1111/j.1365-2761.2008.00914.x 2022-06-13T18:44:45Z Suppression subtractive hybridization was used to investigate the response of turbot, Psetta maxima (L.), to Vibrio harveyi, by using a cDNA library constructed from artificially infected turbot kidney and spleen mRNA. Forty-nine expressed sequence tags were obtained. Several immune system genes were identified, including a major histocompatibility complex (MHC) class Ia gene and a heat shock protein 70 gene. Some signalling molecules were also present in the cDNA libraries, including src-family tyrosine kinase SCK, sgk-1 serine-threonine protein kinase and amyloid precursor-like protein 2. The full length of MHC class Ia cDNA was cloned from turbot cDNA by rapid amplification of cDNA ends polymerase chain reaction. The nucleotide sequence of turbot MHC class Ia has been submitted to GenBank with accession number EF032639. The turbot MHC class Ia cDNA has an open reading frame encoding 354 amino acids, and the deduced amino acid sequence of turbot MHC class Ia has 68%, 54%, 51%, 52%, 57%, 33%, 29% and 29% identities to those of olive flounder, medaka, rainbow trout, Atlantic cod, tiger puffer, chicken, mouse and human, respectively. Quantitative reverse transcriptase-PCR was performed for the MHC class Ia gene, and it was revealed that the expression level of the MHC class Ia gene in V. harveyi-challenged turbot increased to fourfold that of the controls. Article in Journal/Newspaper atlantic cod Turbot University of Stirling: Stirling Digital Research Repository Journal of Fish Diseases 31 7 505 514 |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
immune-related gene MHC class Ia molecular identification quantitative PCR suppression subtractive hybridization |
spellingShingle |
immune-related gene MHC class Ia molecular identification quantitative PCR suppression subtractive hybridization Wang, Chunzai Zhang, Xiao-Hua Jia, A Chen, Jixiang Austin, Brian Identification of immune-related genes from kidney and spleen of turbot, Psetta maxima (L.), by suppression subtractive hybridization following challenge with Vibrio harveyi |
topic_facet |
immune-related gene MHC class Ia molecular identification quantitative PCR suppression subtractive hybridization |
description |
Suppression subtractive hybridization was used to investigate the response of turbot, Psetta maxima (L.), to Vibrio harveyi, by using a cDNA library constructed from artificially infected turbot kidney and spleen mRNA. Forty-nine expressed sequence tags were obtained. Several immune system genes were identified, including a major histocompatibility complex (MHC) class Ia gene and a heat shock protein 70 gene. Some signalling molecules were also present in the cDNA libraries, including src-family tyrosine kinase SCK, sgk-1 serine-threonine protein kinase and amyloid precursor-like protein 2. The full length of MHC class Ia cDNA was cloned from turbot cDNA by rapid amplification of cDNA ends polymerase chain reaction. The nucleotide sequence of turbot MHC class Ia has been submitted to GenBank with accession number EF032639. The turbot MHC class Ia cDNA has an open reading frame encoding 354 amino acids, and the deduced amino acid sequence of turbot MHC class Ia has 68%, 54%, 51%, 52%, 57%, 33%, 29% and 29% identities to those of olive flounder, medaka, rainbow trout, Atlantic cod, tiger puffer, chicken, mouse and human, respectively. Quantitative reverse transcriptase-PCR was performed for the MHC class Ia gene, and it was revealed that the expression level of the MHC class Ia gene in V. harveyi-challenged turbot increased to fourfold that of the controls. |
author2 |
Ocean University of China Heriot-Watt University |
format |
Article in Journal/Newspaper |
author |
Wang, Chunzai Zhang, Xiao-Hua Jia, A Chen, Jixiang Austin, Brian |
author_facet |
Wang, Chunzai Zhang, Xiao-Hua Jia, A Chen, Jixiang Austin, Brian |
author_sort |
Wang, Chunzai |
title |
Identification of immune-related genes from kidney and spleen of turbot, Psetta maxima (L.), by suppression subtractive hybridization following challenge with Vibrio harveyi |
title_short |
Identification of immune-related genes from kidney and spleen of turbot, Psetta maxima (L.), by suppression subtractive hybridization following challenge with Vibrio harveyi |
title_full |
Identification of immune-related genes from kidney and spleen of turbot, Psetta maxima (L.), by suppression subtractive hybridization following challenge with Vibrio harveyi |
title_fullStr |
Identification of immune-related genes from kidney and spleen of turbot, Psetta maxima (L.), by suppression subtractive hybridization following challenge with Vibrio harveyi |
title_full_unstemmed |
Identification of immune-related genes from kidney and spleen of turbot, Psetta maxima (L.), by suppression subtractive hybridization following challenge with Vibrio harveyi |
title_sort |
identification of immune-related genes from kidney and spleen of turbot, psetta maxima (l.), by suppression subtractive hybridization following challenge with vibrio harveyi |
publisher |
Wiley-Blackwell |
publishDate |
2008 |
url |
http://hdl.handle.net/1893/7215 https://doi.org/10.1111/j.1365-2761.2008.00914.x http://dspace.stir.ac.uk/bitstream/1893/7215/1/austin_immune-relatedgenes_2008.pdf |
genre |
atlantic cod Turbot |
genre_facet |
atlantic cod Turbot |
op_relation |
Wang C, Zhang X, Jia A, Chen J & Austin B (2008) Identification of immune-related genes from kidney and spleen of turbot, Psetta maxima (L.), by suppression subtractive hybridization following challenge with Vibrio harveyi. Journal of Fish Diseases, 31 (7), pp. 505-514. https://doi.org/10.1111/j.1365-2761.2008.00914.x http://hdl.handle.net/1893/7215 doi:10.1111/j.1365-2761.2008.00914.x WOS:000256636000004 785712 http://dspace.stir.ac.uk/bitstream/1893/7215/1/austin_immune-relatedgenes_2008.pdf |
op_rights |
The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. http://www.rioxx.net/licenses/under-embargo-all-rights-reserved 3000-01-01 [austin_immune-relatedgenes_2008.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work. |
op_doi |
https://doi.org/10.1111/j.1365-2761.2008.00914.x |
container_title |
Journal of Fish Diseases |
container_volume |
31 |
container_issue |
7 |
container_start_page |
505 |
op_container_end_page |
514 |
_version_ |
1766358100219002880 |