A critical assessment of different transmethylation procedures commonly employed in the fatty acid analysis of aquatic organisms
Several transmethylation procedures have been used for fatty acid analysis of aquatic organisms although the suitability of the applied procedures has rarely been tested. The aim of this study was to demonstrate how different derivatization procedures can affect the result of fatty acid analysis. Di...
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ftunivstirling:oai:dspace.stir.ac.uk:1893/627 2023-05-15T15:32:15+02:00 A critical assessment of different transmethylation procedures commonly employed in the fatty acid analysis of aquatic organisms Schlechtriem, Christian Henderson, R James Tocher, Douglas R Institute of Aquaculture University of Stirling orcid:0000-0002-8603-9410 2008 application/pdf http://hdl.handle.net/1893/627 http://www.aslo.org/lomethods/ http://dspace.stir.ac.uk/bitstream/1893/627/1/Schlechtriem%20et%20al.%202008.pdf en eng American Society of Limnology and Oceanography (ASLO) Schlechtriem C, Henderson RJ & Tocher DR (2008) A critical assessment of different transmethylation procedures commonly employed in the fatty acid analysis of aquatic organisms. Limnology and Oceanography: Methods, 6, pp. 523-531. http://www.aslo.org/lomethods/ http://hdl.handle.net/1893/627 http://www.aslo.org/lomethods/ 2-s2.0-56549130970 836626 http://dspace.stir.ac.uk/bitstream/1893/627/1/Schlechtriem%20et%20al.%202008.pdf Published in Limnology and Oceanography: Methods by ASLO (American Society of Limnology and Oceanography) Fatty acid Analysis Transmethylation methanolic sulphuric acid boron trifluoride comparison Journal Article AM - Accepted Manuscript 2008 ftunivstirling 2022-06-13T18:41:58Z Several transmethylation procedures have been used for fatty acid analysis of aquatic organisms although the suitability of the applied procedures has rarely been tested. The aim of this study was to demonstrate how different derivatization procedures can affect the result of fatty acid analysis. Different transmethylation procedures based on the acidic catalysts boron trifluoride, concentrated sulphuric acid and anhydrous hydrochloric acid were applied to cold-pressed copepod oil and Atlantic salmon flesh lipids rich in wax esters and triacylglycerols, respectively. The results show that 1) the use of unsuitable catalysts and/or incubation conditions may influence the data obtained which can lead to inaccurate conclusions about the presence of fatty acids in aquatic organisms/ecosystems 2) different derivatization procedures based on the same catalyst can produce diverging results and 3) the efficiency of a selected catalyst/procedure should be verified (e.g. by thin-layer chromatography) to ensure the complete transmethylation of fatty acids. Article in Journal/Newspaper Atlantic salmon University of Stirling: Stirling Digital Research Repository |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Fatty acid Analysis Transmethylation methanolic sulphuric acid boron trifluoride comparison |
spellingShingle |
Fatty acid Analysis Transmethylation methanolic sulphuric acid boron trifluoride comparison Schlechtriem, Christian Henderson, R James Tocher, Douglas R A critical assessment of different transmethylation procedures commonly employed in the fatty acid analysis of aquatic organisms |
topic_facet |
Fatty acid Analysis Transmethylation methanolic sulphuric acid boron trifluoride comparison |
description |
Several transmethylation procedures have been used for fatty acid analysis of aquatic organisms although the suitability of the applied procedures has rarely been tested. The aim of this study was to demonstrate how different derivatization procedures can affect the result of fatty acid analysis. Different transmethylation procedures based on the acidic catalysts boron trifluoride, concentrated sulphuric acid and anhydrous hydrochloric acid were applied to cold-pressed copepod oil and Atlantic salmon flesh lipids rich in wax esters and triacylglycerols, respectively. The results show that 1) the use of unsuitable catalysts and/or incubation conditions may influence the data obtained which can lead to inaccurate conclusions about the presence of fatty acids in aquatic organisms/ecosystems 2) different derivatization procedures based on the same catalyst can produce diverging results and 3) the efficiency of a selected catalyst/procedure should be verified (e.g. by thin-layer chromatography) to ensure the complete transmethylation of fatty acids. |
author2 |
Institute of Aquaculture University of Stirling orcid:0000-0002-8603-9410 |
format |
Article in Journal/Newspaper |
author |
Schlechtriem, Christian Henderson, R James Tocher, Douglas R |
author_facet |
Schlechtriem, Christian Henderson, R James Tocher, Douglas R |
author_sort |
Schlechtriem, Christian |
title |
A critical assessment of different transmethylation procedures commonly employed in the fatty acid analysis of aquatic organisms |
title_short |
A critical assessment of different transmethylation procedures commonly employed in the fatty acid analysis of aquatic organisms |
title_full |
A critical assessment of different transmethylation procedures commonly employed in the fatty acid analysis of aquatic organisms |
title_fullStr |
A critical assessment of different transmethylation procedures commonly employed in the fatty acid analysis of aquatic organisms |
title_full_unstemmed |
A critical assessment of different transmethylation procedures commonly employed in the fatty acid analysis of aquatic organisms |
title_sort |
critical assessment of different transmethylation procedures commonly employed in the fatty acid analysis of aquatic organisms |
publisher |
American Society of Limnology and Oceanography (ASLO) |
publishDate |
2008 |
url |
http://hdl.handle.net/1893/627 http://www.aslo.org/lomethods/ http://dspace.stir.ac.uk/bitstream/1893/627/1/Schlechtriem%20et%20al.%202008.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Schlechtriem C, Henderson RJ & Tocher DR (2008) A critical assessment of different transmethylation procedures commonly employed in the fatty acid analysis of aquatic organisms. Limnology and Oceanography: Methods, 6, pp. 523-531. http://www.aslo.org/lomethods/ http://hdl.handle.net/1893/627 http://www.aslo.org/lomethods/ 2-s2.0-56549130970 836626 http://dspace.stir.ac.uk/bitstream/1893/627/1/Schlechtriem%20et%20al.%202008.pdf |
op_rights |
Published in Limnology and Oceanography: Methods by ASLO (American Society of Limnology and Oceanography) |
_version_ |
1766362765135446016 |