Novel DNA-based in situ hybridization method to detect Desmozoon lepeophtherii in Atlantic salmon tissues
The microsporidian Desmozoon lepeophtherii Freeman and Sommerville, 2009 is considered significant in the pathogenesis of gill disease in Atlantic salmon (Salmo salar Linnaeus, 1758). Due to the difficulty in detecting D. lepeophtherii in tissue sections, infections are normally diagnosed by molecul...
Published in: | Journal of Fish Diseases |
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Wiley
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Online Access: | http://hdl.handle.net/1893/34133 https://doi.org/10.1111/jfd.13612 http://dspace.stir.ac.uk/retrieve/cd9014f4-eb62-499d-b6e2-a9d027ebeda9/Herrero-etal-JFD-2022.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/34133 2023-05-15T15:30:46+02:00 Novel DNA-based in situ hybridization method to detect Desmozoon lepeophtherii in Atlantic salmon tissues Herrero, Ana Palenzuela, Oswaldo Rodger, Hamish Matthews, Chris Marcos-Lopez, Mar Bron, James E Dagleish, Mark P Thompson, Kim D European Commission (Horizon 2020) The Moredun Research Institute Institute of Aquaculture Torre de la Sal VAI Consulting PHARMAQ Institute of Aquaculture orcid:0000-0003-3544-0519 2022-03-30 application/pdf http://hdl.handle.net/1893/34133 https://doi.org/10.1111/jfd.13612 http://dspace.stir.ac.uk/retrieve/cd9014f4-eb62-499d-b6e2-a9d027ebeda9/Herrero-etal-JFD-2022.pdf en eng Wiley Herrero A, Palenzuela O, Rodger H, Matthews C, Marcos-Lopez M, Bron JE, Dagleish MP & Thompson KD (2022) Novel DNA-based in situ hybridization method to detect Desmozoon lepeophtherii in Atlantic salmon tissues. Journal of Fish Diseases. https://doi.org/10.1111/jfd.13612 AquaExcel2020 652831 http://hdl.handle.net/1893/34133 doi:10.1111/jfd.13612 35352838 1807479 http://dspace.stir.ac.uk/retrieve/cd9014f4-eb62-499d-b6e2-a9d027ebeda9/Herrero-etal-JFD-2022.pdf This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. 2023-03-31 [Herrero-etal-JFD-2022.pdf] Publisher requires embargo of 12 months after publication. Desmozoon lepeophtherii gill disease ISH microsporidian Paranucleospora theridion Journal Article AM - Accepted Manuscript 2022 ftunivstirling https://doi.org/10.1111/jfd.13612 2022-06-13T18:43:53Z The microsporidian Desmozoon lepeophtherii Freeman and Sommerville, 2009 is considered significant in the pathogenesis of gill disease in Atlantic salmon (Salmo salar Linnaeus, 1758). Due to the difficulty in detecting D. lepeophtherii in tissue sections, infections are normally diagnosed by molecular methods, routine haematoxylin and eosin (H&E) stained gill tissue sections and the use of other histochemical stains and labels to confirm the presence of spores. An in situ hybridization (ISH) protocol specific for D. lepeophtherii was developed using DIG-labelled oligonucleotide probes. Diseased Atlantic salmon gills were analysed by ISH, calcofluor white (CW) and H&E. All methods showed high levels of specificity (100%) in their ability to detect D. lepeophtherii, but the sensitivity was higher with ISH (92%), compared with CW (64%) and the presence of microvesicles on H&E stained sections (52%). High levels of D. lepeophtherii spores were significantly associated (p < .05) with the development of D. lepeophtherii-associated pathology in the gills, with Ct values below 19 and over 100 microsporidia/10 mm2 of gill tissue (from the ISH counts) seemingly necessary for the development of microvesicles. The ISH method has the advantage over other histological techniques in that it allows all life stages of the microsporidian to be detected in infected salmon gill tissue sections. Output Status: Forthcoming/Available Online Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Journal of Fish Diseases |
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University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Desmozoon lepeophtherii gill disease ISH microsporidian Paranucleospora theridion |
spellingShingle |
Desmozoon lepeophtherii gill disease ISH microsporidian Paranucleospora theridion Herrero, Ana Palenzuela, Oswaldo Rodger, Hamish Matthews, Chris Marcos-Lopez, Mar Bron, James E Dagleish, Mark P Thompson, Kim D Novel DNA-based in situ hybridization method to detect Desmozoon lepeophtherii in Atlantic salmon tissues |
topic_facet |
Desmozoon lepeophtherii gill disease ISH microsporidian Paranucleospora theridion |
description |
The microsporidian Desmozoon lepeophtherii Freeman and Sommerville, 2009 is considered significant in the pathogenesis of gill disease in Atlantic salmon (Salmo salar Linnaeus, 1758). Due to the difficulty in detecting D. lepeophtherii in tissue sections, infections are normally diagnosed by molecular methods, routine haematoxylin and eosin (H&E) stained gill tissue sections and the use of other histochemical stains and labels to confirm the presence of spores. An in situ hybridization (ISH) protocol specific for D. lepeophtherii was developed using DIG-labelled oligonucleotide probes. Diseased Atlantic salmon gills were analysed by ISH, calcofluor white (CW) and H&E. All methods showed high levels of specificity (100%) in their ability to detect D. lepeophtherii, but the sensitivity was higher with ISH (92%), compared with CW (64%) and the presence of microvesicles on H&E stained sections (52%). High levels of D. lepeophtherii spores were significantly associated (p < .05) with the development of D. lepeophtherii-associated pathology in the gills, with Ct values below 19 and over 100 microsporidia/10 mm2 of gill tissue (from the ISH counts) seemingly necessary for the development of microvesicles. The ISH method has the advantage over other histological techniques in that it allows all life stages of the microsporidian to be detected in infected salmon gill tissue sections. Output Status: Forthcoming/Available Online |
author2 |
European Commission (Horizon 2020) The Moredun Research Institute Institute of Aquaculture Torre de la Sal VAI Consulting PHARMAQ Institute of Aquaculture orcid:0000-0003-3544-0519 |
format |
Article in Journal/Newspaper |
author |
Herrero, Ana Palenzuela, Oswaldo Rodger, Hamish Matthews, Chris Marcos-Lopez, Mar Bron, James E Dagleish, Mark P Thompson, Kim D |
author_facet |
Herrero, Ana Palenzuela, Oswaldo Rodger, Hamish Matthews, Chris Marcos-Lopez, Mar Bron, James E Dagleish, Mark P Thompson, Kim D |
author_sort |
Herrero, Ana |
title |
Novel DNA-based in situ hybridization method to detect Desmozoon lepeophtherii in Atlantic salmon tissues |
title_short |
Novel DNA-based in situ hybridization method to detect Desmozoon lepeophtherii in Atlantic salmon tissues |
title_full |
Novel DNA-based in situ hybridization method to detect Desmozoon lepeophtherii in Atlantic salmon tissues |
title_fullStr |
Novel DNA-based in situ hybridization method to detect Desmozoon lepeophtherii in Atlantic salmon tissues |
title_full_unstemmed |
Novel DNA-based in situ hybridization method to detect Desmozoon lepeophtherii in Atlantic salmon tissues |
title_sort |
novel dna-based in situ hybridization method to detect desmozoon lepeophtherii in atlantic salmon tissues |
publisher |
Wiley |
publishDate |
2022 |
url |
http://hdl.handle.net/1893/34133 https://doi.org/10.1111/jfd.13612 http://dspace.stir.ac.uk/retrieve/cd9014f4-eb62-499d-b6e2-a9d027ebeda9/Herrero-etal-JFD-2022.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Herrero A, Palenzuela O, Rodger H, Matthews C, Marcos-Lopez M, Bron JE, Dagleish MP & Thompson KD (2022) Novel DNA-based in situ hybridization method to detect Desmozoon lepeophtherii in Atlantic salmon tissues. Journal of Fish Diseases. https://doi.org/10.1111/jfd.13612 AquaExcel2020 652831 http://hdl.handle.net/1893/34133 doi:10.1111/jfd.13612 35352838 1807479 http://dspace.stir.ac.uk/retrieve/cd9014f4-eb62-499d-b6e2-a9d027ebeda9/Herrero-etal-JFD-2022.pdf |
op_rights |
This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. 2023-03-31 [Herrero-etal-JFD-2022.pdf] Publisher requires embargo of 12 months after publication. |
op_doi |
https://doi.org/10.1111/jfd.13612 |
container_title |
Journal of Fish Diseases |
_version_ |
1766361213815488512 |