Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery
Ballan wrasse (Labrus bergylta, Ascanius 1767) are cleaner fish cultured in northern Europe to remove sea lice from farmed Atlantic salmon (Salmo salar, Linnaeus 1758). Despite increasing appreciation for the importance of the microbiota on the phenotypes of vertebrates including teleosts, the micro...
Published in: | Current Microbiology |
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Main Authors: | , , , , , , |
Other Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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BMC
2021
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Online Access: | http://hdl.handle.net/1893/32051 https://doi.org/10.1007/s00284-020-02286-8 http://dspace.stir.ac.uk/bitstream/1893/32051/1/Bone2021_Article_BacterialCommunitiesOfBallanWr.pdf |
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Open Polar |
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University of Stirling: Stirling Digital Research Repository |
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ftunivstirling |
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English |
description |
Ballan wrasse (Labrus bergylta, Ascanius 1767) are cleaner fish cultured in northern Europe to remove sea lice from farmed Atlantic salmon (Salmo salar, Linnaeus 1758). Despite increasing appreciation for the importance of the microbiota on the phenotypes of vertebrates including teleosts, the microbiota of wrasse eggs has yet to be described. Therefore, the aim of this present study was to describe the bacterial component of the microbiota of ballan wrasse eggs shortly after spawning and at 5 days, once the eggs had undergone a routine incubation protocol that included surface disinfection steps in a common holding tank. Triplicate egg samples were collected from each of three spawning tanks and analysis of 16S rRNA gene sequences revealed that 88.6% of reads could be identified to 186 taxonomic families. At Day 0, reads corresponding to members of the Vibrionaceae, Colwelliaceae and Rubritaleaceae families were detected at greatest relative abundances. Bacterial communities of eggs varied more greatly between tanks than between samples deriving from the same tank. At Day 5, there was a consistent reduction in 16S rRNA gene sequence richness across the tanks. Even though the eggs from the different tanks were incubated in a common holding tank, the bacterial communities of the eggs from the different tanks had diverged to become increasingly dissimilar. This suggests that the disinfection and incubation exerted differential effects of the microbiota of the eggs from each tank and that the influence of the tank water on the composition of the egg microbiota was lower than expected. This first comprehensive description of the ballan wrasse egg bacterial community is an initial step to understand the role and function of the microbiota on the phenotype of this fish. In future, mass DNA sequencing methods may be applied in hatcheries to screen for pathogens and as a tool to assess the health status of eggs. |
author2 |
Scottish Aquaculture Innovation Centre Institute of Aquaculture orcid:0000-0002-1206-7654 orcid:0000-0003-2368-7864 orcid:0000-0002-9524-618X orcid:0000-0001-6052-8761 |
format |
Article in Journal/Newspaper |
author |
Bone, Aileen Bekaert, Michaël Papadopoulou, Athina McMillan, Stuart Adams, Alexandra Davie, Andrew Desbois, Andrew P |
spellingShingle |
Bone, Aileen Bekaert, Michaël Papadopoulou, Athina McMillan, Stuart Adams, Alexandra Davie, Andrew Desbois, Andrew P Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery |
author_facet |
Bone, Aileen Bekaert, Michaël Papadopoulou, Athina McMillan, Stuart Adams, Alexandra Davie, Andrew Desbois, Andrew P |
author_sort |
Bone, Aileen |
title |
Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery |
title_short |
Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery |
title_full |
Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery |
title_fullStr |
Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery |
title_full_unstemmed |
Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery |
title_sort |
bacterial communities of ballan wrasse (labrus bergylta) eggs at a commercial marine hatchery |
publisher |
BMC |
publishDate |
2021 |
url |
http://hdl.handle.net/1893/32051 https://doi.org/10.1007/s00284-020-02286-8 http://dspace.stir.ac.uk/bitstream/1893/32051/1/Bone2021_Article_BacterialCommunitiesOfBallanWr.pdf |
long_lat |
ENVELOPE(12.203,12.203,65.945,65.945) |
geographic |
Ballan |
geographic_facet |
Ballan |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Bone A, Bekaert M, Papadopoulou A, McMillan S, Adams A, Davie A & Desbois AP (2021) Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery. Current Microbiology, 78 (1), pp. 114-124. https://doi.org/10.1007/s00284-020-02286-8 http://hdl.handle.net/1893/32051 doi:10.1007/s00284-020-02286-8 33230621 WOS:000591986600002 2-s2.0-85096440949 1686740 http://dspace.stir.ac.uk/bitstream/1893/32051/1/Bone2021_Article_BacterialCommunitiesOfBallanWr.pdf |
op_rights |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00284-020-02286-8 |
container_title |
Current Microbiology |
container_volume |
78 |
container_issue |
1 |
container_start_page |
114 |
op_container_end_page |
124 |
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1766363392807796736 |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/32051 2023-05-15T15:32:55+02:00 Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery Bone, Aileen Bekaert, Michaël Papadopoulou, Athina McMillan, Stuart Adams, Alexandra Davie, Andrew Desbois, Andrew P Scottish Aquaculture Innovation Centre Institute of Aquaculture orcid:0000-0002-1206-7654 orcid:0000-0003-2368-7864 orcid:0000-0002-9524-618X orcid:0000-0001-6052-8761 2021-01 application/pdf http://hdl.handle.net/1893/32051 https://doi.org/10.1007/s00284-020-02286-8 http://dspace.stir.ac.uk/bitstream/1893/32051/1/Bone2021_Article_BacterialCommunitiesOfBallanWr.pdf en eng BMC Bone A, Bekaert M, Papadopoulou A, McMillan S, Adams A, Davie A & Desbois AP (2021) Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery. Current Microbiology, 78 (1), pp. 114-124. https://doi.org/10.1007/s00284-020-02286-8 http://hdl.handle.net/1893/32051 doi:10.1007/s00284-020-02286-8 33230621 WOS:000591986600002 2-s2.0-85096440949 1686740 http://dspace.stir.ac.uk/bitstream/1893/32051/1/Bone2021_Article_BacterialCommunitiesOfBallanWr.pdf This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. http://creativecommons.org/licenses/by/4.0/ CC-BY Journal Article VoR - Version of Record 2021 ftunivstirling https://doi.org/10.1007/s00284-020-02286-8 2022-06-13T18:44:56Z Ballan wrasse (Labrus bergylta, Ascanius 1767) are cleaner fish cultured in northern Europe to remove sea lice from farmed Atlantic salmon (Salmo salar, Linnaeus 1758). Despite increasing appreciation for the importance of the microbiota on the phenotypes of vertebrates including teleosts, the microbiota of wrasse eggs has yet to be described. Therefore, the aim of this present study was to describe the bacterial component of the microbiota of ballan wrasse eggs shortly after spawning and at 5 days, once the eggs had undergone a routine incubation protocol that included surface disinfection steps in a common holding tank. Triplicate egg samples were collected from each of three spawning tanks and analysis of 16S rRNA gene sequences revealed that 88.6% of reads could be identified to 186 taxonomic families. At Day 0, reads corresponding to members of the Vibrionaceae, Colwelliaceae and Rubritaleaceae families were detected at greatest relative abundances. Bacterial communities of eggs varied more greatly between tanks than between samples deriving from the same tank. At Day 5, there was a consistent reduction in 16S rRNA gene sequence richness across the tanks. Even though the eggs from the different tanks were incubated in a common holding tank, the bacterial communities of the eggs from the different tanks had diverged to become increasingly dissimilar. This suggests that the disinfection and incubation exerted differential effects of the microbiota of the eggs from each tank and that the influence of the tank water on the composition of the egg microbiota was lower than expected. This first comprehensive description of the ballan wrasse egg bacterial community is an initial step to understand the role and function of the microbiota on the phenotype of this fish. In future, mass DNA sequencing methods may be applied in hatcheries to screen for pathogens and as a tool to assess the health status of eggs. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Ballan ENVELOPE(12.203,12.203,65.945,65.945) Current Microbiology 78 1 114 124 |