A commercial autogenous injection vaccine protects ballan wrasse (Labrus bergylta, ascanius) against aeromonas salmonicida vapA type V
Atypical Aeromonas salmonicida (aAs) and Vibrionaceae related species are bacteria routinely recovered from diseased ballan wrasse used as cleaner fish in Atlantic salmon farming. Autogenous (i.e. farm specific inactivated) multivalent vaccines formulated from these microorganisms are widely used by...
Published in: | Fish & Shellfish Immunology |
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Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier
2020
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Subjects: | |
Online Access: | http://hdl.handle.net/1893/31868 https://doi.org/10.1016/j.fsi.2020.09.040 http://dspace.stir.ac.uk/bitstream/1893/31868/1/Ramirez-Paredes-etal-Accepted%20manuscript-2020.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/31868 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
ballan wrasse vaccines bacterial diseases atypical Aeromonas salmonicida cleaner fish diseases vibriosis in wrasse Photobacterium indicum |
spellingShingle |
ballan wrasse vaccines bacterial diseases atypical Aeromonas salmonicida cleaner fish diseases vibriosis in wrasse Photobacterium indicum Ramirez-Paredes, J Gustavo Verner-Jeffreys, David Papadopoulou, Athina Monaghan, Sean J Smith, Louise Haydon, David Wallis, Tim S Davie, Andrew Adams, Alexandra Migaud, Herve A commercial autogenous injection vaccine protects ballan wrasse (Labrus bergylta, ascanius) against aeromonas salmonicida vapA type V |
topic_facet |
ballan wrasse vaccines bacterial diseases atypical Aeromonas salmonicida cleaner fish diseases vibriosis in wrasse Photobacterium indicum |
description |
Atypical Aeromonas salmonicida (aAs) and Vibrionaceae related species are bacteria routinely recovered from diseased ballan wrasse used as cleaner fish in Atlantic salmon farming. Autogenous (i.e. farm specific inactivated) multivalent vaccines formulated from these microorganisms are widely used by the industry to protect farmed wrasse despite limited experimental proof that they are primary pathogens. In this study, the components of a commercial multivalent injection vaccine containing four strains of Aeromonas salmonicida and one strain of Vibrio splendidus previously isolated from ballan wrasse in Scotland, were tested for infectivity, pathogenicity and virulence via intra peritoneal injection at pre-deployment size (25-50g) and the efficacy of the vaccine for protection against aAs assessed. Injection with 3.5x109, 8x109 1.8x109 and 5x109 cfu/fish of Vibrio splendidus, V. ichthyoenteri, Aliivibrio logeii and A. salmonicida, respectively, did not cause significant mortalities, lesions or clinical signs after a period of 14 days. IP injection with both aAs and Photobacterium indicum successfully reproduced the clinical signs and internal lesions observed during natural outbreaks of the disease. Differences in virulence (LD50 at day 8-post infection of 3.6x106 cfu/fish and 1.6x107 cfu/fish) were observed for two aAs vapA type V isolates. In addition, the LD50 for Photobacterium indicum was 2.2x107 cfu/fish. The autogenous vaccine was highly protective against the two aAs vapA type V isolates after 700-degree days of immunisation. The RPSFINAL values for the first isolate were 95 and 91% at 1x106 cfu/fish and 1x107 cfu/fish, respectively, and 79% at 1x107 cfu/fish for the second isolate tested. In addition, significantly higher anti aAs seral antibodies (IgM), were detected by ELISA in vaccinated fish in contrast with control (mock vaccinated) fish. These results suggest wrasse can be effectively immunised and protected against aAs infection by injection with oil adjuvanted vaccines prepared with inactivated ... |
author2 |
Scottish Aquaculture Innovation Centre Ridgeway Biologicals Ltd CEFAS - Centre for Environment, Fisheries and Aquaculture Science Institute of Aquaculture orcid:0000-0002-7692-7756 orcid:0000-0002-9524-618X orcid:0000-0002-5404-7512 |
format |
Article in Journal/Newspaper |
author |
Ramirez-Paredes, J Gustavo Verner-Jeffreys, David Papadopoulou, Athina Monaghan, Sean J Smith, Louise Haydon, David Wallis, Tim S Davie, Andrew Adams, Alexandra Migaud, Herve |
author_facet |
Ramirez-Paredes, J Gustavo Verner-Jeffreys, David Papadopoulou, Athina Monaghan, Sean J Smith, Louise Haydon, David Wallis, Tim S Davie, Andrew Adams, Alexandra Migaud, Herve |
author_sort |
Ramirez-Paredes, J Gustavo |
title |
A commercial autogenous injection vaccine protects ballan wrasse (Labrus bergylta, ascanius) against aeromonas salmonicida vapA type V |
title_short |
A commercial autogenous injection vaccine protects ballan wrasse (Labrus bergylta, ascanius) against aeromonas salmonicida vapA type V |
title_full |
A commercial autogenous injection vaccine protects ballan wrasse (Labrus bergylta, ascanius) against aeromonas salmonicida vapA type V |
title_fullStr |
A commercial autogenous injection vaccine protects ballan wrasse (Labrus bergylta, ascanius) against aeromonas salmonicida vapA type V |
title_full_unstemmed |
A commercial autogenous injection vaccine protects ballan wrasse (Labrus bergylta, ascanius) against aeromonas salmonicida vapA type V |
title_sort |
commercial autogenous injection vaccine protects ballan wrasse (labrus bergylta, ascanius) against aeromonas salmonicida vapa type v |
publisher |
Elsevier |
publishDate |
2020 |
url |
http://hdl.handle.net/1893/31868 https://doi.org/10.1016/j.fsi.2020.09.040 http://dspace.stir.ac.uk/bitstream/1893/31868/1/Ramirez-Paredes-etal-Accepted%20manuscript-2020.pdf |
long_lat |
ENVELOPE(12.203,12.203,65.945,65.945) |
geographic |
Ballan |
geographic_facet |
Ballan |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Ramirez-Paredes JG, Verner-Jeffreys D, Papadopoulou A, Monaghan SJ, Smith L, Haydon D, Wallis TS, Davie A, Adams A & Migaud H (2020) A commercial autogenous injection vaccine protects ballan wrasse (Labrus bergylta, ascanius) against aeromonas salmonicida vapA type V. Fish and Shellfish Immunology, 107 (Part A), pp. 43-53. https://doi.org/10.1016/j.fsi.2020.09.040 http://hdl.handle.net/1893/31868 doi:10.1016/j.fsi.2020.09.040 33011432 WOS:000599882200006 2-s2.0-85092504353 1668747 http://dspace.stir.ac.uk/bitstream/1893/31868/1/Ramirez-Paredes-etal-Accepted%20manuscript-2020.pdf |
op_rights |
This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Ramirez-Paredes JG, Verner-Jeffreys D, Papadopoulou A, Monaghan SJ, Smith L, Haydon D, Wallis TS, Davie A, Adams A & Migaud H (2020) A commercial autogenous injection vaccine protects ballan wrasse (Labrus bergylta, ascanius) against aeromonas salmonicida vapA type V. Fish and Shellfish Immunology, 107 (Part A), pp. 43-53. https://doi.org/10.1016/j.fsi.2020.09.040 © 2020, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ 2021-10-02 [Ramirez-Paredes-etal-Accepted manuscript-2020.pdf] Publisher requires embargo of 12 months after formal publication. |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.fsi.2020.09.040 |
container_title |
Fish & Shellfish Immunology |
container_volume |
107 |
container_start_page |
43 |
op_container_end_page |
53 |
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1766363392050724864 |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/31868 2023-05-15T15:32:55+02:00 A commercial autogenous injection vaccine protects ballan wrasse (Labrus bergylta, ascanius) against aeromonas salmonicida vapA type V Ramirez-Paredes, J Gustavo Verner-Jeffreys, David Papadopoulou, Athina Monaghan, Sean J Smith, Louise Haydon, David Wallis, Tim S Davie, Andrew Adams, Alexandra Migaud, Herve Scottish Aquaculture Innovation Centre Ridgeway Biologicals Ltd CEFAS - Centre for Environment, Fisheries and Aquaculture Science Institute of Aquaculture orcid:0000-0002-7692-7756 orcid:0000-0002-9524-618X orcid:0000-0002-5404-7512 2020-12 application/pdf http://hdl.handle.net/1893/31868 https://doi.org/10.1016/j.fsi.2020.09.040 http://dspace.stir.ac.uk/bitstream/1893/31868/1/Ramirez-Paredes-etal-Accepted%20manuscript-2020.pdf en eng Elsevier Ramirez-Paredes JG, Verner-Jeffreys D, Papadopoulou A, Monaghan SJ, Smith L, Haydon D, Wallis TS, Davie A, Adams A & Migaud H (2020) A commercial autogenous injection vaccine protects ballan wrasse (Labrus bergylta, ascanius) against aeromonas salmonicida vapA type V. Fish and Shellfish Immunology, 107 (Part A), pp. 43-53. https://doi.org/10.1016/j.fsi.2020.09.040 http://hdl.handle.net/1893/31868 doi:10.1016/j.fsi.2020.09.040 33011432 WOS:000599882200006 2-s2.0-85092504353 1668747 http://dspace.stir.ac.uk/bitstream/1893/31868/1/Ramirez-Paredes-etal-Accepted%20manuscript-2020.pdf This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Ramirez-Paredes JG, Verner-Jeffreys D, Papadopoulou A, Monaghan SJ, Smith L, Haydon D, Wallis TS, Davie A, Adams A & Migaud H (2020) A commercial autogenous injection vaccine protects ballan wrasse (Labrus bergylta, ascanius) against aeromonas salmonicida vapA type V. Fish and Shellfish Immunology, 107 (Part A), pp. 43-53. https://doi.org/10.1016/j.fsi.2020.09.040 © 2020, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ 2021-10-02 [Ramirez-Paredes-etal-Accepted manuscript-2020.pdf] Publisher requires embargo of 12 months after formal publication. CC-BY-NC-ND ballan wrasse vaccines bacterial diseases atypical Aeromonas salmonicida cleaner fish diseases vibriosis in wrasse Photobacterium indicum Journal Article AM - Accepted Manuscript 2020 ftunivstirling https://doi.org/10.1016/j.fsi.2020.09.040 2022-06-13T18:44:17Z Atypical Aeromonas salmonicida (aAs) and Vibrionaceae related species are bacteria routinely recovered from diseased ballan wrasse used as cleaner fish in Atlantic salmon farming. Autogenous (i.e. farm specific inactivated) multivalent vaccines formulated from these microorganisms are widely used by the industry to protect farmed wrasse despite limited experimental proof that they are primary pathogens. In this study, the components of a commercial multivalent injection vaccine containing four strains of Aeromonas salmonicida and one strain of Vibrio splendidus previously isolated from ballan wrasse in Scotland, were tested for infectivity, pathogenicity and virulence via intra peritoneal injection at pre-deployment size (25-50g) and the efficacy of the vaccine for protection against aAs assessed. Injection with 3.5x109, 8x109 1.8x109 and 5x109 cfu/fish of Vibrio splendidus, V. ichthyoenteri, Aliivibrio logeii and A. salmonicida, respectively, did not cause significant mortalities, lesions or clinical signs after a period of 14 days. IP injection with both aAs and Photobacterium indicum successfully reproduced the clinical signs and internal lesions observed during natural outbreaks of the disease. Differences in virulence (LD50 at day 8-post infection of 3.6x106 cfu/fish and 1.6x107 cfu/fish) were observed for two aAs vapA type V isolates. In addition, the LD50 for Photobacterium indicum was 2.2x107 cfu/fish. The autogenous vaccine was highly protective against the two aAs vapA type V isolates after 700-degree days of immunisation. The RPSFINAL values for the first isolate were 95 and 91% at 1x106 cfu/fish and 1x107 cfu/fish, respectively, and 79% at 1x107 cfu/fish for the second isolate tested. In addition, significantly higher anti aAs seral antibodies (IgM), were detected by ELISA in vaccinated fish in contrast with control (mock vaccinated) fish. These results suggest wrasse can be effectively immunised and protected against aAs infection by injection with oil adjuvanted vaccines prepared with inactivated ... Article in Journal/Newspaper Atlantic salmon University of Stirling: Stirling Digital Research Repository Ballan ENVELOPE(12.203,12.203,65.945,65.945) Fish & Shellfish Immunology 107 43 53 |