The role of Desmozoon lepeophtherii in complex gill disorder of Atlantic salmon (Salmo salar)
Gill disease is an important challenge for Atlantic salmon (Salmo salar L.) aquaculture worldwide. Complex gill disorder (CGD) is a multifactorial and multiaetiological condition that tends to occur from late summer to early winter in salmon. The microsporidian Desmozoon lepeophtherii has been assoc...
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Language: | English |
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University of Stirling
2019
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Online Access: | http://hdl.handle.net/1893/31807 http://dspace.stir.ac.uk/bitstream/1893/31807/1/Herrero%20A.-%20The%20role%20of%20Desmozoon%20lepeophtherii%20in%20complex%20gill%20disorder%20of%20%20%20%20%20%20Atlantic%20salmon.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/31807 2023-05-15T15:30:53+02:00 The role of Desmozoon lepeophtherii in complex gill disorder of Atlantic salmon (Salmo salar) Herrero-Fernandez, Ana Thompson, Kim Dagleish, Mark Bron, James Adams, Alexandra Rodger, Hamish Matthews, Chris Moredun Research Institue & Fish Vet Group 2019-09 application/pdf http://hdl.handle.net/1893/31807 http://dspace.stir.ac.uk/bitstream/1893/31807/1/Herrero%20A.-%20The%20role%20of%20Desmozoon%20lepeophtherii%20in%20complex%20gill%20disorder%20of%20%20%20%20%20%20Atlantic%20salmon.pdf en eng University of Stirling http://hdl.handle.net/1893/31807 http://dspace.stir.ac.uk/bitstream/1893/31807/1/Herrero%20A.-%20The%20role%20of%20Desmozoon%20lepeophtherii%20in%20complex%20gill%20disorder%20of%20%20%20%20%20%20Atlantic%20salmon.pdf 2020-12-29 Articles still in the process of publication 2020-12-30 Desmozoon lepeophtherii Paranucleospora theridion Atlantic salmon Complex gill disorder Complex gill disease Gill disease Microsporidia Branchiomonas cysticola Salmon gill poxvirus Paramoeba perurans Longitudinal study Cell culture In situ hybridization Fishes Diseases Thesis or Dissertation Doctoral Doctor of Philosophy 2019 ftunivstirling 2022-06-13T18:44:53Z Gill disease is an important challenge for Atlantic salmon (Salmo salar L.) aquaculture worldwide. Complex gill disorder (CGD) is a multifactorial and multiaetiological condition that tends to occur from late summer to early winter in salmon. The microsporidian Desmozoon lepeophtherii has been associated with CGD, but the interaction between the pathogen and its host remains to be understood. This thesis examines different aspects of D. lepeophtherii in an attempt to clarify the role and significance of D. lepeophtherii in CGD. Spores from D. lepeophtherii, derived from the sea lice (Lepeophtheirus salmonis) were used to infect different fish cell lines (rainbow trout gill cells and salmon head kindey cells) and primary macrophage cultures from Atlantic salmon head kidney in vitro. However, there was no evidence of D. lepeophtherii replication in any of the cultures. A one-year longitudinal study was carried out at two marine salmon farms to determine the correlation between gill pathology and the putative pathogens associated with CGD (D. lepeophtherii, Candidatus Branchiomonas cysticola and salmon gill pox virus (SGPV)), as well as Paramoeba perurans, the aetiological agent of amoebic gill disease (AGD). The two farms were positive for the four pathogens, with Ca. B. cysticola and D. lepeophtherii being the most frequently detected agents, and SGPV detected sporadically throughout the study. Paramoeba perurans was detected in the two farms but an outbreak of AGD only occurred in one of the farms. Statistical analysis of the data from the two farms showed that variations in SGPV and Ca. B. cysticola loads were not associated with an increase in the gill score (p>0.05), while D. lepeophtherii and P. perurans were (p< 0.001), although obvious pathology associated with D. lepeophtherii infection was not evident. An in situ hybridisation (ISH) method was developed to detect the developmental and spore stages of the parasite, the sensitivity (92%) of which was higher than other staining methods currently used ... Doctoral or Postdoctoral Thesis Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Desmozoon lepeophtherii Paranucleospora theridion Atlantic salmon Complex gill disorder Complex gill disease Gill disease Microsporidia Branchiomonas cysticola Salmon gill poxvirus Paramoeba perurans Longitudinal study Cell culture In situ hybridization Fishes Diseases |
spellingShingle |
Desmozoon lepeophtherii Paranucleospora theridion Atlantic salmon Complex gill disorder Complex gill disease Gill disease Microsporidia Branchiomonas cysticola Salmon gill poxvirus Paramoeba perurans Longitudinal study Cell culture In situ hybridization Fishes Diseases Herrero-Fernandez, Ana The role of Desmozoon lepeophtherii in complex gill disorder of Atlantic salmon (Salmo salar) |
topic_facet |
Desmozoon lepeophtherii Paranucleospora theridion Atlantic salmon Complex gill disorder Complex gill disease Gill disease Microsporidia Branchiomonas cysticola Salmon gill poxvirus Paramoeba perurans Longitudinal study Cell culture In situ hybridization Fishes Diseases |
description |
Gill disease is an important challenge for Atlantic salmon (Salmo salar L.) aquaculture worldwide. Complex gill disorder (CGD) is a multifactorial and multiaetiological condition that tends to occur from late summer to early winter in salmon. The microsporidian Desmozoon lepeophtherii has been associated with CGD, but the interaction between the pathogen and its host remains to be understood. This thesis examines different aspects of D. lepeophtherii in an attempt to clarify the role and significance of D. lepeophtherii in CGD. Spores from D. lepeophtherii, derived from the sea lice (Lepeophtheirus salmonis) were used to infect different fish cell lines (rainbow trout gill cells and salmon head kindey cells) and primary macrophage cultures from Atlantic salmon head kidney in vitro. However, there was no evidence of D. lepeophtherii replication in any of the cultures. A one-year longitudinal study was carried out at two marine salmon farms to determine the correlation between gill pathology and the putative pathogens associated with CGD (D. lepeophtherii, Candidatus Branchiomonas cysticola and salmon gill pox virus (SGPV)), as well as Paramoeba perurans, the aetiological agent of amoebic gill disease (AGD). The two farms were positive for the four pathogens, with Ca. B. cysticola and D. lepeophtherii being the most frequently detected agents, and SGPV detected sporadically throughout the study. Paramoeba perurans was detected in the two farms but an outbreak of AGD only occurred in one of the farms. Statistical analysis of the data from the two farms showed that variations in SGPV and Ca. B. cysticola loads were not associated with an increase in the gill score (p>0.05), while D. lepeophtherii and P. perurans were (p< 0.001), although obvious pathology associated with D. lepeophtherii infection was not evident. An in situ hybridisation (ISH) method was developed to detect the developmental and spore stages of the parasite, the sensitivity (92%) of which was higher than other staining methods currently used ... |
author2 |
Thompson, Kim Dagleish, Mark Bron, James Adams, Alexandra Rodger, Hamish Matthews, Chris Moredun Research Institue & Fish Vet Group |
format |
Doctoral or Postdoctoral Thesis |
author |
Herrero-Fernandez, Ana |
author_facet |
Herrero-Fernandez, Ana |
author_sort |
Herrero-Fernandez, Ana |
title |
The role of Desmozoon lepeophtherii in complex gill disorder of Atlantic salmon (Salmo salar) |
title_short |
The role of Desmozoon lepeophtherii in complex gill disorder of Atlantic salmon (Salmo salar) |
title_full |
The role of Desmozoon lepeophtherii in complex gill disorder of Atlantic salmon (Salmo salar) |
title_fullStr |
The role of Desmozoon lepeophtherii in complex gill disorder of Atlantic salmon (Salmo salar) |
title_full_unstemmed |
The role of Desmozoon lepeophtherii in complex gill disorder of Atlantic salmon (Salmo salar) |
title_sort |
role of desmozoon lepeophtherii in complex gill disorder of atlantic salmon (salmo salar) |
publisher |
University of Stirling |
publishDate |
2019 |
url |
http://hdl.handle.net/1893/31807 http://dspace.stir.ac.uk/bitstream/1893/31807/1/Herrero%20A.-%20The%20role%20of%20Desmozoon%20lepeophtherii%20in%20complex%20gill%20disorder%20of%20%20%20%20%20%20Atlantic%20salmon.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://hdl.handle.net/1893/31807 http://dspace.stir.ac.uk/bitstream/1893/31807/1/Herrero%20A.-%20The%20role%20of%20Desmozoon%20lepeophtherii%20in%20complex%20gill%20disorder%20of%20%20%20%20%20%20Atlantic%20salmon.pdf |
op_rights |
2020-12-29 Articles still in the process of publication 2020-12-30 |
_version_ |
1766361354632953856 |