A comparison of the use of different swab materials for optimal diagnosis of amoebic gill disease (AGD) in Atlantic salmon (Salmo salar L.)
Routine gill swabbing is a non‐destructive sampling method used for the downstream qPCR detection and quantitation of the pathogen Neoparamoeba perurans, a causative agent of amoebic gill disease (AGD). Three commercially available swabs were compared aiming their application for timelier AGD diagno...
Published in: | Journal of Fish Diseases |
---|---|
Main Authors: | , , , , , , , , |
Other Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2020
|
Subjects: | |
Online Access: | http://hdl.handle.net/1893/31643 https://doi.org/10.1111/jfd.13243 http://dspace.stir.ac.uk/bitstream/1893/31643/1/jfd.13243.pdf |
id |
ftunivstirling:oai:dspace.stir.ac.uk:1893/31643 |
---|---|
record_format |
openpolar |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/31643 2023-05-15T15:32:36+02:00 A comparison of the use of different swab materials for optimal diagnosis of amoebic gill disease (AGD) in Atlantic salmon (Salmo salar L.) Fernandez‐Senac, Carolina Fridman, Sophie Sokolowska, Jadwiga Monaghan, Sean J. Garzon, Teresa Betancor, Monica Paladini, Giuseppe Adams, Alexandra Bron, James E. Horizon 2020 (Outputs) Institute of Aquaculture Mowi (Scotland) orcid:0000-0001-5896-8186 orcid:0000-0002-0159-0474 orcid:0000-0002-7692-7756 orcid:0000-0003-1626-7458 orcid:0000-0003-4944-0499 orcid:0000-0003-3544-0519 2020-11 application/pdf http://hdl.handle.net/1893/31643 https://doi.org/10.1111/jfd.13243 http://dspace.stir.ac.uk/bitstream/1893/31643/1/jfd.13243.pdf en eng Wiley Fernandez‐Senac C, Fridman S, Sokolowska J, Monaghan SJ, Garzon T, Betancor M, Paladini G, Adams A & Bron JE (2020) A comparison of the use of different swab materials for optimal diagnosis of amoebic gill disease (AGD) in Atlantic salmon (Salmo salar L.). Journal of Fish Diseases, 43 (11), pp. 1463-1472. https://doi.org/10.1111/jfd.13243 http://hdl.handle.net/1893/31643 doi:10.1111/jfd.13243 32882753 WOS:000565430300001 2-s2.0-85090156685 1658293 http://dspace.stir.ac.uk/bitstream/1893/31643/1/jfd.13243.pdf © 2020 The Authors. Journal of Fish Diseases published by John Wiley & Sons Ltd This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. http://creativecommons.org/licenses/by-nc-nd/4.0/ CC-BY-NC-ND amoeba calcium alginate diagnostics sodium citrate Journal Article VoR - Version of Record 2020 ftunivstirling https://doi.org/10.1111/jfd.13243 2022-06-13T18:42:20Z Routine gill swabbing is a non‐destructive sampling method used for the downstream qPCR detection and quantitation of the pathogen Neoparamoeba perurans, a causative agent of amoebic gill disease (AGD). Three commercially available swabs were compared aiming their application for timelier AGD diagnosis (Calgiswab® (calcium alginate fibre‐tipped), Isohelix® DNA buccal and cotton wool‐tipped). Calcium alginate is soluble in most sodium salts, which potentially allows the total recovery of biological material, hence a better extraction of target organisms’ DNA. Thus, this study consisted of (a) an in vitro assessment involving spiking of the swabs with known amounts of amoebae and additional assessment of retrieval efficiency of amoebae from agar plates; (b) in vivo testing by swabbing of gill arches (second, third and fourth) of AGD‐infected fish. Both in vitro and in vivo experiments identified an enhanced amoeba retrieval with Calgiswab® and Isohelix® swabs in comparison with cotton swabs. Additionally, the third and fourth gill arches presented significantly higher amoebic loads compared to the second gill arch. Results suggest that limiting routine gill swabbing to one or two arches, instead of all, could likely lead to reduced stress‐related effects incurred by handling and sampling and a timelier diagnosis of AGD. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Journal of Fish Diseases 43 11 1463 1472 |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
amoeba calcium alginate diagnostics sodium citrate |
spellingShingle |
amoeba calcium alginate diagnostics sodium citrate Fernandez‐Senac, Carolina Fridman, Sophie Sokolowska, Jadwiga Monaghan, Sean J. Garzon, Teresa Betancor, Monica Paladini, Giuseppe Adams, Alexandra Bron, James E. A comparison of the use of different swab materials for optimal diagnosis of amoebic gill disease (AGD) in Atlantic salmon (Salmo salar L.) |
topic_facet |
amoeba calcium alginate diagnostics sodium citrate |
description |
Routine gill swabbing is a non‐destructive sampling method used for the downstream qPCR detection and quantitation of the pathogen Neoparamoeba perurans, a causative agent of amoebic gill disease (AGD). Three commercially available swabs were compared aiming their application for timelier AGD diagnosis (Calgiswab® (calcium alginate fibre‐tipped), Isohelix® DNA buccal and cotton wool‐tipped). Calcium alginate is soluble in most sodium salts, which potentially allows the total recovery of biological material, hence a better extraction of target organisms’ DNA. Thus, this study consisted of (a) an in vitro assessment involving spiking of the swabs with known amounts of amoebae and additional assessment of retrieval efficiency of amoebae from agar plates; (b) in vivo testing by swabbing of gill arches (second, third and fourth) of AGD‐infected fish. Both in vitro and in vivo experiments identified an enhanced amoeba retrieval with Calgiswab® and Isohelix® swabs in comparison with cotton swabs. Additionally, the third and fourth gill arches presented significantly higher amoebic loads compared to the second gill arch. Results suggest that limiting routine gill swabbing to one or two arches, instead of all, could likely lead to reduced stress‐related effects incurred by handling and sampling and a timelier diagnosis of AGD. |
author2 |
Horizon 2020 (Outputs) Institute of Aquaculture Mowi (Scotland) orcid:0000-0001-5896-8186 orcid:0000-0002-0159-0474 orcid:0000-0002-7692-7756 orcid:0000-0003-1626-7458 orcid:0000-0003-4944-0499 orcid:0000-0003-3544-0519 |
format |
Article in Journal/Newspaper |
author |
Fernandez‐Senac, Carolina Fridman, Sophie Sokolowska, Jadwiga Monaghan, Sean J. Garzon, Teresa Betancor, Monica Paladini, Giuseppe Adams, Alexandra Bron, James E. |
author_facet |
Fernandez‐Senac, Carolina Fridman, Sophie Sokolowska, Jadwiga Monaghan, Sean J. Garzon, Teresa Betancor, Monica Paladini, Giuseppe Adams, Alexandra Bron, James E. |
author_sort |
Fernandez‐Senac, Carolina |
title |
A comparison of the use of different swab materials for optimal diagnosis of amoebic gill disease (AGD) in Atlantic salmon (Salmo salar L.) |
title_short |
A comparison of the use of different swab materials for optimal diagnosis of amoebic gill disease (AGD) in Atlantic salmon (Salmo salar L.) |
title_full |
A comparison of the use of different swab materials for optimal diagnosis of amoebic gill disease (AGD) in Atlantic salmon (Salmo salar L.) |
title_fullStr |
A comparison of the use of different swab materials for optimal diagnosis of amoebic gill disease (AGD) in Atlantic salmon (Salmo salar L.) |
title_full_unstemmed |
A comparison of the use of different swab materials for optimal diagnosis of amoebic gill disease (AGD) in Atlantic salmon (Salmo salar L.) |
title_sort |
comparison of the use of different swab materials for optimal diagnosis of amoebic gill disease (agd) in atlantic salmon (salmo salar l.) |
publisher |
Wiley |
publishDate |
2020 |
url |
http://hdl.handle.net/1893/31643 https://doi.org/10.1111/jfd.13243 http://dspace.stir.ac.uk/bitstream/1893/31643/1/jfd.13243.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Fernandez‐Senac C, Fridman S, Sokolowska J, Monaghan SJ, Garzon T, Betancor M, Paladini G, Adams A & Bron JE (2020) A comparison of the use of different swab materials for optimal diagnosis of amoebic gill disease (AGD) in Atlantic salmon (Salmo salar L.). Journal of Fish Diseases, 43 (11), pp. 1463-1472. https://doi.org/10.1111/jfd.13243 http://hdl.handle.net/1893/31643 doi:10.1111/jfd.13243 32882753 WOS:000565430300001 2-s2.0-85090156685 1658293 http://dspace.stir.ac.uk/bitstream/1893/31643/1/jfd.13243.pdf |
op_rights |
© 2020 The Authors. Journal of Fish Diseases published by John Wiley & Sons Ltd This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1111/jfd.13243 |
container_title |
Journal of Fish Diseases |
container_volume |
43 |
container_issue |
11 |
container_start_page |
1463 |
op_container_end_page |
1472 |
_version_ |
1766363089066786816 |