Biosynthesis of very long-chain fatty acids (C > 24) in Atlantic salmon: Cloning, functional characterisation, and tissue distribution of an Elovl4 elongase
The elongases of very long-chain fatty acids (Elovl) account for the rate-limiting condensation step of the elongation process in fatty acid (FA) biosynthesis in vertebrates. One member of the Elovl family, Elovl4, has been regarded as a critical enzyme in vertebrates in the production of the so-cal...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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Main Authors: | , , , , |
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier
2011
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Online Access: | http://hdl.handle.net/1893/3156 https://doi.org/10.1016/j.cbpb.2011.02.007 http://www.sciencedirect.com/science/journal/10964959 http://dspace.stir.ac.uk/bitstream/1893/3156/1/Carmona%20et%20al%20%28FINAL%29.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/3156 |
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openpolar |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Atlantic salmon fatty acid metabolism Very long chain polyunsaturated fatty acid biosynthesis Elovl4 elongase cDNA cloning Functional characterisation Yeast Fishes Feeding and feeds Fishes Nutrition Lipids in nutrition |
spellingShingle |
Atlantic salmon fatty acid metabolism Very long chain polyunsaturated fatty acid biosynthesis Elovl4 elongase cDNA cloning Functional characterisation Yeast Fishes Feeding and feeds Fishes Nutrition Lipids in nutrition Carmona-Antonanzas, Greta Monroig, Oscar Dick, James R Davie, Andrew Tocher, Douglas R Biosynthesis of very long-chain fatty acids (C > 24) in Atlantic salmon: Cloning, functional characterisation, and tissue distribution of an Elovl4 elongase |
topic_facet |
Atlantic salmon fatty acid metabolism Very long chain polyunsaturated fatty acid biosynthesis Elovl4 elongase cDNA cloning Functional characterisation Yeast Fishes Feeding and feeds Fishes Nutrition Lipids in nutrition |
description |
The elongases of very long-chain fatty acids (Elovl) account for the rate-limiting condensation step of the elongation process in fatty acid (FA) biosynthesis in vertebrates. One member of the Elovl family, Elovl4, has been regarded as a critical enzyme in vertebrates in the production of the so-called very long-chain fatty acids (VLC-FA), a group of compounds that have been scarcely explored in fish. Here we report on the cloning of a novel elovl4-like elongase from Atlantic salmon. The salmon elovl4 cDNA codes for a putative protein containing 306 amino acids. Heterologous expression in yeast demonstrated that salmon Elovl4 efficiently elongated saturated FAs up to 36:0, with 24:0 and 26:0 appearing as preferred substrates. Additionally, salmon Elovl4 effectively converted C20 and C22 polyunsaturated fatty acids to elongated polyenoic products up to C36. Tissue distribution showed that elovl4 mRNA transcripts are abundant in eye, brain and testes, suggesting that, as described in mammals, these tissues are important metabolic sites for the biosynthesis of VLC-FA. Our results are discussed in comparison with the functional analyses observed in Elovl4 proteins from other vertebrates, and also other Elovl proteins investigated previously in Atlantic salmon. |
author2 |
European Commission Institute of Aquaculture orcid:0000-0001-8712-0440 orcid:0000-0002-9524-618X orcid:0000-0002-8603-9410 |
format |
Article in Journal/Newspaper |
author |
Carmona-Antonanzas, Greta Monroig, Oscar Dick, James R Davie, Andrew Tocher, Douglas R |
author_facet |
Carmona-Antonanzas, Greta Monroig, Oscar Dick, James R Davie, Andrew Tocher, Douglas R |
author_sort |
Carmona-Antonanzas, Greta |
title |
Biosynthesis of very long-chain fatty acids (C > 24) in Atlantic salmon: Cloning, functional characterisation, and tissue distribution of an Elovl4 elongase |
title_short |
Biosynthesis of very long-chain fatty acids (C > 24) in Atlantic salmon: Cloning, functional characterisation, and tissue distribution of an Elovl4 elongase |
title_full |
Biosynthesis of very long-chain fatty acids (C > 24) in Atlantic salmon: Cloning, functional characterisation, and tissue distribution of an Elovl4 elongase |
title_fullStr |
Biosynthesis of very long-chain fatty acids (C > 24) in Atlantic salmon: Cloning, functional characterisation, and tissue distribution of an Elovl4 elongase |
title_full_unstemmed |
Biosynthesis of very long-chain fatty acids (C > 24) in Atlantic salmon: Cloning, functional characterisation, and tissue distribution of an Elovl4 elongase |
title_sort |
biosynthesis of very long-chain fatty acids (c > 24) in atlantic salmon: cloning, functional characterisation, and tissue distribution of an elovl4 elongase |
publisher |
Elsevier |
publishDate |
2011 |
url |
http://hdl.handle.net/1893/3156 https://doi.org/10.1016/j.cbpb.2011.02.007 http://www.sciencedirect.com/science/journal/10964959 http://dspace.stir.ac.uk/bitstream/1893/3156/1/Carmona%20et%20al%20%28FINAL%29.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Carmona-Antonanzas G, Monroig O, Dick JR, Davie A & Tocher DR (2011) Biosynthesis of very long-chain fatty acids (C > 24) in Atlantic salmon: Cloning, functional characterisation, and tissue distribution of an Elovl4 elongase. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 159 (2), pp. 122-129. http://www.sciencedirect.com/science/journal/10964959; https://doi.org/10.1016/j.cbpb.2011.02.007 Marie Curie IEF Fishinutrigen COBIAGENE. PIEF-GA-2008-220929 PIEF-GA-2008-219667 http://hdl.handle.net/1893/3156 doi:10.1016/j.cbpb.2011.02.007 http://www.sciencedirect.com/science/journal/10964959 WOS:000289917100008 2-s2.0-79953329052 837303 http://dspace.stir.ac.uk/bitstream/1893/3156/1/Carmona%20et%20al%20%28FINAL%29.pdf |
op_rights |
Published in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology by Elsevier. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Volume 159, Issue 2, June 2011, pp. 122 - 129.; This is the peer reviewed version of this article.; NOTICE: this is the author’s version of a work that was accepted for publication in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, VOL 159, ISSUE 2, (June 2011). DOI:10.1016/j.cbpb.2011.02.007. |
op_doi |
https://doi.org/10.1016/j.cbpb.2011.02.007 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
159 |
container_issue |
2 |
container_start_page |
122 |
op_container_end_page |
129 |
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1766360732706799616 |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/3156 2023-05-15T15:30:17+02:00 Biosynthesis of very long-chain fatty acids (C > 24) in Atlantic salmon: Cloning, functional characterisation, and tissue distribution of an Elovl4 elongase Carmona-Antonanzas, Greta Monroig, Oscar Dick, James R Davie, Andrew Tocher, Douglas R European Commission Institute of Aquaculture orcid:0000-0001-8712-0440 orcid:0000-0002-9524-618X orcid:0000-0002-8603-9410 2011-06 application/pdf http://hdl.handle.net/1893/3156 https://doi.org/10.1016/j.cbpb.2011.02.007 http://www.sciencedirect.com/science/journal/10964959 http://dspace.stir.ac.uk/bitstream/1893/3156/1/Carmona%20et%20al%20%28FINAL%29.pdf en eng Elsevier Carmona-Antonanzas G, Monroig O, Dick JR, Davie A & Tocher DR (2011) Biosynthesis of very long-chain fatty acids (C > 24) in Atlantic salmon: Cloning, functional characterisation, and tissue distribution of an Elovl4 elongase. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 159 (2), pp. 122-129. http://www.sciencedirect.com/science/journal/10964959; https://doi.org/10.1016/j.cbpb.2011.02.007 Marie Curie IEF Fishinutrigen COBIAGENE. PIEF-GA-2008-220929 PIEF-GA-2008-219667 http://hdl.handle.net/1893/3156 doi:10.1016/j.cbpb.2011.02.007 http://www.sciencedirect.com/science/journal/10964959 WOS:000289917100008 2-s2.0-79953329052 837303 http://dspace.stir.ac.uk/bitstream/1893/3156/1/Carmona%20et%20al%20%28FINAL%29.pdf Published in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology by Elsevier. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Volume 159, Issue 2, June 2011, pp. 122 - 129.; This is the peer reviewed version of this article.; NOTICE: this is the author’s version of a work that was accepted for publication in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, VOL 159, ISSUE 2, (June 2011). DOI:10.1016/j.cbpb.2011.02.007. Atlantic salmon fatty acid metabolism Very long chain polyunsaturated fatty acid biosynthesis Elovl4 elongase cDNA cloning Functional characterisation Yeast Fishes Feeding and feeds Fishes Nutrition Lipids in nutrition Journal Article AM - Accepted Manuscript 2011 ftunivstirling https://doi.org/10.1016/j.cbpb.2011.02.007 2022-06-13T18:44:13Z The elongases of very long-chain fatty acids (Elovl) account for the rate-limiting condensation step of the elongation process in fatty acid (FA) biosynthesis in vertebrates. One member of the Elovl family, Elovl4, has been regarded as a critical enzyme in vertebrates in the production of the so-called very long-chain fatty acids (VLC-FA), a group of compounds that have been scarcely explored in fish. Here we report on the cloning of a novel elovl4-like elongase from Atlantic salmon. The salmon elovl4 cDNA codes for a putative protein containing 306 amino acids. Heterologous expression in yeast demonstrated that salmon Elovl4 efficiently elongated saturated FAs up to 36:0, with 24:0 and 26:0 appearing as preferred substrates. Additionally, salmon Elovl4 effectively converted C20 and C22 polyunsaturated fatty acids to elongated polyenoic products up to C36. Tissue distribution showed that elovl4 mRNA transcripts are abundant in eye, brain and testes, suggesting that, as described in mammals, these tissues are important metabolic sites for the biosynthesis of VLC-FA. Our results are discussed in comparison with the functional analyses observed in Elovl4 proteins from other vertebrates, and also other Elovl proteins investigated previously in Atlantic salmon. Article in Journal/Newspaper Atlantic salmon University of Stirling: Stirling Digital Research Repository Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 159 2 122 129 |