Short term cold storage and sperm concentration assessment of lumpfish (Cyclopterus lumpus. L) Milt
There is increased commercial interest in the production of lumpfish (Cyclopterus lumpus) as a biological control against sea lice infections in Atlantic salmon farming. To ensure a sustainable supply of lumpfish, reliable captive reproduction is required however, optimal husbandry and conditions fo...
Published in: | Aquaculture |
---|---|
Main Authors: | , , |
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier BV
2020
|
Subjects: | |
Online Access: | http://hdl.handle.net/1893/31413 https://doi.org/10.1016/j.aquaculture.2020.735646 http://dspace.stir.ac.uk/bitstream/1893/31413/1/Lumpfish%20milt%20paper%20STORRE.pdf |
id |
ftunivstirling:oai:dspace.stir.ac.uk:1893/31413 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Lumpfish Cleaner fish Milt extenders Sperm motility Sperm concentration Spermatocrit |
spellingShingle |
Lumpfish Cleaner fish Milt extenders Sperm motility Sperm concentration Spermatocrit Pountney, Samuel M Migaud, Herve Davie, Andrew Short term cold storage and sperm concentration assessment of lumpfish (Cyclopterus lumpus. L) Milt |
topic_facet |
Lumpfish Cleaner fish Milt extenders Sperm motility Sperm concentration Spermatocrit |
description |
There is increased commercial interest in the production of lumpfish (Cyclopterus lumpus) as a biological control against sea lice infections in Atlantic salmon farming. To ensure a sustainable supply of lumpfish, reliable captive reproduction is required however, optimal husbandry and conditions for captive broodstock performance remain unknown. Artificial fertilisation of gametes remains the preferred management strategy for lumpfish, but this requires an effective milt management protocol. The present study tested several milt extender solutions for short term cold storage and validated sperm concentration assessment of lumpfish milt. Results demonstrated lumpfish sperm has a long motility survival time (motile for up to 3 h) when compared to other marine teleosts. Importantly, all extenders used in the study were non-activating on dilution of the milt. Lumpfish milt was successfully stored at 4 °C in Modified Turbot Extender (MTE), Herring Ringers Solution and Aquaboost SpermCoat (Cryogenetics, Norway) for up to 14 days post stripping. MTE performed more effectively with regards to maintenance of sperm activation time in comparison to the other tested extenders. There was a significant positive correlation between sperm concentration identified through cell counts using a haemocytometer and both packed cell volume (spermatocrit) and measured optical density at 640 nm (r2 = 97.42 ± 2.14). This suggests both packed cell volume and spectrophotometric measurements can be effective methods for rapidly assessing sperm concentration in lumpfish. This study validated several options for quantifying sperm concentration and short-term cold storage of lumpfish milt that can be used for hatchery management in lumpfish aquaculture. |
author2 |
SAIC Scottish Aquaculture Innovation Centre Institute of Aquaculture orcid:0000-0003-0952-839X orcid:0000-0002-5404-7512 orcid:0000-0002-9524-618X |
format |
Article in Journal/Newspaper |
author |
Pountney, Samuel M Migaud, Herve Davie, Andrew |
author_facet |
Pountney, Samuel M Migaud, Herve Davie, Andrew |
author_sort |
Pountney, Samuel M |
title |
Short term cold storage and sperm concentration assessment of lumpfish (Cyclopterus lumpus. L) Milt |
title_short |
Short term cold storage and sperm concentration assessment of lumpfish (Cyclopterus lumpus. L) Milt |
title_full |
Short term cold storage and sperm concentration assessment of lumpfish (Cyclopterus lumpus. L) Milt |
title_fullStr |
Short term cold storage and sperm concentration assessment of lumpfish (Cyclopterus lumpus. L) Milt |
title_full_unstemmed |
Short term cold storage and sperm concentration assessment of lumpfish (Cyclopterus lumpus. L) Milt |
title_sort |
short term cold storage and sperm concentration assessment of lumpfish (cyclopterus lumpus. l) milt |
publisher |
Elsevier BV |
publishDate |
2020 |
url |
http://hdl.handle.net/1893/31413 https://doi.org/10.1016/j.aquaculture.2020.735646 http://dspace.stir.ac.uk/bitstream/1893/31413/1/Lumpfish%20milt%20paper%20STORRE.pdf |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon Turbot |
genre_facet |
Atlantic salmon Turbot |
op_relation |
Pountney SM, Migaud H & Davie A (2020) Short term cold storage and sperm concentration assessment of lumpfish (Cyclopterus lumpus. L) Milt. Aquaculture, 529, Art. No.: 735646. https://doi.org/10.1016/j.aquaculture.2020.735646 Securing a sustainable supply and the optimal deployment of lumpsucker for sea lice control in the Scottish salmon industry SL_2015_03 735646 http://hdl.handle.net/1893/31413 doi:10.1016/j.aquaculture.2020.735646 WOS:000566466700017 2-s2.0-85087916466 1643613 http://dspace.stir.ac.uk/bitstream/1893/31413/1/Lumpfish%20milt%20paper%20STORRE.pdf |
op_rights |
This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Pountney SM, Migaud H & Davie A (2020) Short term cold storage and sperm concentration assessment of lumpfish (Cyclopterus lumpus. L) Milt. Aquaculture, 529, Art. No.: 735646. https://doi.org/10.1016/j.aquaculture.2020.735646 © 2020, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ 2021-07-01 [Lumpfish milt paper STORRE.pdf] Publisher requires embargo of 12 months after formal publication. |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.aquaculture.2020.735646 |
container_title |
Aquaculture |
container_volume |
529 |
container_start_page |
735646 |
_version_ |
1766363187210354688 |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/31413 2023-05-15T15:32:42+02:00 Short term cold storage and sperm concentration assessment of lumpfish (Cyclopterus lumpus. L) Milt Pountney, Samuel M Migaud, Herve Davie, Andrew SAIC Scottish Aquaculture Innovation Centre Institute of Aquaculture orcid:0000-0003-0952-839X orcid:0000-0002-5404-7512 orcid:0000-0002-9524-618X 2020-12-15 application/pdf http://hdl.handle.net/1893/31413 https://doi.org/10.1016/j.aquaculture.2020.735646 http://dspace.stir.ac.uk/bitstream/1893/31413/1/Lumpfish%20milt%20paper%20STORRE.pdf en eng Elsevier BV Pountney SM, Migaud H & Davie A (2020) Short term cold storage and sperm concentration assessment of lumpfish (Cyclopterus lumpus. L) Milt. Aquaculture, 529, Art. No.: 735646. https://doi.org/10.1016/j.aquaculture.2020.735646 Securing a sustainable supply and the optimal deployment of lumpsucker for sea lice control in the Scottish salmon industry SL_2015_03 735646 http://hdl.handle.net/1893/31413 doi:10.1016/j.aquaculture.2020.735646 WOS:000566466700017 2-s2.0-85087916466 1643613 http://dspace.stir.ac.uk/bitstream/1893/31413/1/Lumpfish%20milt%20paper%20STORRE.pdf This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Pountney SM, Migaud H & Davie A (2020) Short term cold storage and sperm concentration assessment of lumpfish (Cyclopterus lumpus. L) Milt. Aquaculture, 529, Art. No.: 735646. https://doi.org/10.1016/j.aquaculture.2020.735646 © 2020, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ 2021-07-01 [Lumpfish milt paper STORRE.pdf] Publisher requires embargo of 12 months after formal publication. CC-BY-NC-ND Lumpfish Cleaner fish Milt extenders Sperm motility Sperm concentration Spermatocrit Journal Article AM - Accepted Manuscript 2020 ftunivstirling https://doi.org/10.1016/j.aquaculture.2020.735646 2022-06-13T18:43:59Z There is increased commercial interest in the production of lumpfish (Cyclopterus lumpus) as a biological control against sea lice infections in Atlantic salmon farming. To ensure a sustainable supply of lumpfish, reliable captive reproduction is required however, optimal husbandry and conditions for captive broodstock performance remain unknown. Artificial fertilisation of gametes remains the preferred management strategy for lumpfish, but this requires an effective milt management protocol. The present study tested several milt extender solutions for short term cold storage and validated sperm concentration assessment of lumpfish milt. Results demonstrated lumpfish sperm has a long motility survival time (motile for up to 3 h) when compared to other marine teleosts. Importantly, all extenders used in the study were non-activating on dilution of the milt. Lumpfish milt was successfully stored at 4 °C in Modified Turbot Extender (MTE), Herring Ringers Solution and Aquaboost SpermCoat (Cryogenetics, Norway) for up to 14 days post stripping. MTE performed more effectively with regards to maintenance of sperm activation time in comparison to the other tested extenders. There was a significant positive correlation between sperm concentration identified through cell counts using a haemocytometer and both packed cell volume (spermatocrit) and measured optical density at 640 nm (r2 = 97.42 ± 2.14). This suggests both packed cell volume and spectrophotometric measurements can be effective methods for rapidly assessing sperm concentration in lumpfish. This study validated several options for quantifying sperm concentration and short-term cold storage of lumpfish milt that can be used for hatchery management in lumpfish aquaculture. Article in Journal/Newspaper Atlantic salmon Turbot University of Stirling: Stirling Digital Research Repository Norway Aquaculture 529 735646 |